The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos

Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan...

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Autores principales: Mazzini, María Nélida, Cerezo, Alberto Saúl
Publicado: 1986
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v6_n3_p203_Mazzini
http://hdl.handle.net/20.500.12110/paper_01448617_v6_n3_p203_Mazzini
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_01448617_v6_n3_p203_Mazzini2023-06-08T15:12:10Z The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos Mazzini, María Nélida Cerezo, Alberto Saúl Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan-protein complex which was further purified by anion-exchange chromatography. The galactomannan has a medium degree of polymerization and a backbone similar to that of the classical galactomannans. The main difference with these polysaccharides lies in the side-chains which are attached to the backbone not only by (1 → 6)-linkages but also by (1 → 3)-linkages and which contain, on average, two galactose units, either (1 → 3), (1 → 6)-linked or (1 → 3), (1 → 3)-linked with α-d and β-d configurations, respectively. © 1986. Fil:Mazzini, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v6_n3_p203_Mazzini http://hdl.handle.net/20.500.12110/paper_01448617_v6_n3_p203_Mazzini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan-protein complex which was further purified by anion-exchange chromatography. The galactomannan has a medium degree of polymerization and a backbone similar to that of the classical galactomannans. The main difference with these polysaccharides lies in the side-chains which are attached to the backbone not only by (1 → 6)-linkages but also by (1 → 3)-linkages and which contain, on average, two galactose units, either (1 → 3), (1 → 6)-linked or (1 → 3), (1 → 3)-linked with α-d and β-d configurations, respectively. © 1986.
author Mazzini, María Nélida
Cerezo, Alberto Saúl
spellingShingle Mazzini, María Nélida
Cerezo, Alberto Saúl
The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
author_facet Mazzini, María Nélida
Cerezo, Alberto Saúl
author_sort Mazzini, María Nélida
title The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
title_short The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
title_full The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
title_fullStr The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
title_full_unstemmed The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos
title_sort alkali-soluble galactomannan-like oligosaccharides from the seed of gleditsia triacanthos
publishDate 1986
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v6_n3_p203_Mazzini
http://hdl.handle.net/20.500.12110/paper_01448617_v6_n3_p203_Mazzini
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