Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS
Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstruct...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v179_n_p402_TraffanoSchiffo http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo |
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paper:paper_01448617_v179_n_p402_TraffanoSchiffo2023-06-08T15:12:04Z Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstructure due to the constraints imposed by the operational conditions. The aim of this research is to study the time course of microstructural changes of Ca(II)-alginate matrices driven by the presence of trehalose, arabic and guar gums as excipients and to discuss how these changes influence the diffusional transport (assessed by LF-NMR) and the enzymatic activity of the encapsulated lactase. The structural modifications at different scales were assessed by SAXS. The incorporation of gums as second excipients induces a significant stabilization in the microstructure not only at the rod scale, but also in the characteristic size and density of alginate dimers (basic units of construction of rods) and the degree of interconnection of rods at a larger scale, improving the performance in terms of lactase activity. © 2017 Elsevier Ltd 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v179_n_p402_TraffanoSchiffo http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction |
| spellingShingle |
Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| topic_facet |
Alginate beads Encapsulation Hydrocolloids Microstructure Small-Angle X-ray scattering (SAXS) β-Galactosidase Calcium Dehydration Encapsulation Microstructure X ray scattering Alginate beads Alginate encapsulation Galactosidases Hydrocolloids Microstructural changes Microstructure stability Operational conditions Structural modifications Alginate alginic acid excipient galactan glucuronic acid guar gum gum arabic hexuronic acid immobilized enzyme lactase mannan nitrobenzene derivative plant gum trehalose chemistry diffusion hydrogel metabolism small angle scattering time factor X ray diffraction Alginates Diffusion Enzymes, Immobilized Excipients Galactans Glucuronic Acid Gum Arabic Hexuronic Acids Hydrogels Lactase Mannans Nitrobenzenes Plant Gums Scattering, Small Angle Time Factors Trehalose X-Ray Diffraction |
| description |
Previous works show that the addition of trehalose and gums in β-galactosidase (lactase) Ca(II)-alginate encapsulation systems improved its intrinsic stability against freezing and dehydration processes in the pristine state. However, there is no available information on the evolution in microstructure due to the constraints imposed by the operational conditions. The aim of this research is to study the time course of microstructural changes of Ca(II)-alginate matrices driven by the presence of trehalose, arabic and guar gums as excipients and to discuss how these changes influence the diffusional transport (assessed by LF-NMR) and the enzymatic activity of the encapsulated lactase. The structural modifications at different scales were assessed by SAXS. The incorporation of gums as second excipients induces a significant stabilization in the microstructure not only at the rod scale, but also in the characteristic size and density of alginate dimers (basic units of construction of rods) and the degree of interconnection of rods at a larger scale, improving the performance in terms of lactase activity. © 2017 Elsevier Ltd |
| title |
Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| title_short |
Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| title_full |
Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| title_fullStr |
Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| title_full_unstemmed |
Gums induced microstructure stability in Ca(II)-alginate beads containing lactase analyzed by SAXS |
| title_sort |
gums induced microstructure stability in ca(ii)-alginate beads containing lactase analyzed by saxs |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v179_n_p402_TraffanoSchiffo http://hdl.handle.net/20.500.12110/paper_01448617_v179_n_p402_TraffanoSchiffo |
| _version_ |
1768546202489454592 |