Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells
Introduction Pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in the placenta, where it functions as an autocrine hormone. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation o...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01434004_v57_n_p152_Schanton http://hdl.handle.net/20.500.12110/paper_01434004_v57_n_p152_Schanton |
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paper:paper_01434004_v57_n_p152_Schanton2023-06-08T15:11:51Z Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells Maymó, Julieta L. Gambino, Yésica Paola Varone, Cecilia Laura Estradiol Leptin expression Placenta Sp1 transcription factor estradiol estrogen receptor alpha fulvestrant leptin small interfering RNA transcription factor Sp1 estradiol estrogen receptor alpha leptin transcription factor Sp1 Article BeWo cell line choriocarcinoma cell line controlled study down regulation gene expression human human cell placenta priority journal quantitative analysis reverse transcription polymerase chain reaction transient transfection Western blotting analogs and derivatives female metabolism physiology placenta pregnancy tumor cell line Cell Line, Tumor Estradiol Estrogen Receptor alpha Female Humans Leptin Placenta Pregnancy Sp1 Transcription Factor Introduction Pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in the placenta, where it functions as an autocrine hormone. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol up-regulates placental leptin expression through genomic and nongenomic mechanisms. Methods To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, we examined Sp1 transcription factor effect on estradiol leptin induction in human BeWo cell line. Results We demonstrated that Sp1 induces leptin expression determined by qRT-PCR, Western blot and transient transfection experiments. We also found that estradiol induction effect on leptin expression is enhanced by the over expression of Sp1 factor. Moreover, estradiol effect was not evidenced when Sp1 binding site on leptin promoter is mutated, suggesting that estradiol action is dependent on Sp1. On the other hand we showed data that demonstrate that Sp1 induction of leptin expression is insensitive to the antiestrogen ICI 182 780. By over expression experiments, we have also found that Sp1 effect on leptin expression could be mediated by estrogen receptor alpha. Supporting this idea, the downregulation of estrogen receptor alpha level through a specific siRNA, abolished Sp1 effect on leptin expression. Discussion Taken together all these evidences suggest a cooperative behavior between estrogen receptor alpha and Sp1 transcription factors to induce leptin transcription. © 2017 Elsevier Ltd Fil:Maymó, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gambino, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01434004_v57_n_p152_Schanton http://hdl.handle.net/20.500.12110/paper_01434004_v57_n_p152_Schanton |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Estradiol Leptin expression Placenta Sp1 transcription factor estradiol estrogen receptor alpha fulvestrant leptin small interfering RNA transcription factor Sp1 estradiol estrogen receptor alpha leptin transcription factor Sp1 Article BeWo cell line choriocarcinoma cell line controlled study down regulation gene expression human human cell placenta priority journal quantitative analysis reverse transcription polymerase chain reaction transient transfection Western blotting analogs and derivatives female metabolism physiology placenta pregnancy tumor cell line Cell Line, Tumor Estradiol Estrogen Receptor alpha Female Humans Leptin Placenta Pregnancy Sp1 Transcription Factor |
| spellingShingle |
Estradiol Leptin expression Placenta Sp1 transcription factor estradiol estrogen receptor alpha fulvestrant leptin small interfering RNA transcription factor Sp1 estradiol estrogen receptor alpha leptin transcription factor Sp1 Article BeWo cell line choriocarcinoma cell line controlled study down regulation gene expression human human cell placenta priority journal quantitative analysis reverse transcription polymerase chain reaction transient transfection Western blotting analogs and derivatives female metabolism physiology placenta pregnancy tumor cell line Cell Line, Tumor Estradiol Estrogen Receptor alpha Female Humans Leptin Placenta Pregnancy Sp1 Transcription Factor Maymó, Julieta L. Gambino, Yésica Paola Varone, Cecilia Laura Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| topic_facet |
Estradiol Leptin expression Placenta Sp1 transcription factor estradiol estrogen receptor alpha fulvestrant leptin small interfering RNA transcription factor Sp1 estradiol estrogen receptor alpha leptin transcription factor Sp1 Article BeWo cell line choriocarcinoma cell line controlled study down regulation gene expression human human cell placenta priority journal quantitative analysis reverse transcription polymerase chain reaction transient transfection Western blotting analogs and derivatives female metabolism physiology placenta pregnancy tumor cell line Cell Line, Tumor Estradiol Estrogen Receptor alpha Female Humans Leptin Placenta Pregnancy Sp1 Transcription Factor |
| description |
Introduction Pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in the placenta, where it functions as an autocrine hormone. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol up-regulates placental leptin expression through genomic and nongenomic mechanisms. Methods To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, we examined Sp1 transcription factor effect on estradiol leptin induction in human BeWo cell line. Results We demonstrated that Sp1 induces leptin expression determined by qRT-PCR, Western blot and transient transfection experiments. We also found that estradiol induction effect on leptin expression is enhanced by the over expression of Sp1 factor. Moreover, estradiol effect was not evidenced when Sp1 binding site on leptin promoter is mutated, suggesting that estradiol action is dependent on Sp1. On the other hand we showed data that demonstrate that Sp1 induction of leptin expression is insensitive to the antiestrogen ICI 182 780. By over expression experiments, we have also found that Sp1 effect on leptin expression could be mediated by estrogen receptor alpha. Supporting this idea, the downregulation of estrogen receptor alpha level through a specific siRNA, abolished Sp1 effect on leptin expression. Discussion Taken together all these evidences suggest a cooperative behavior between estrogen receptor alpha and Sp1 transcription factors to induce leptin transcription. © 2017 Elsevier Ltd |
| author |
Maymó, Julieta L. Gambino, Yésica Paola Varone, Cecilia Laura |
| author_facet |
Maymó, Julieta L. Gambino, Yésica Paola Varone, Cecilia Laura |
| author_sort |
Maymó, Julieta L. |
| title |
Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| title_short |
Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| title_full |
Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| title_fullStr |
Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| title_full_unstemmed |
Sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| title_sort |
sp1 transcription factor is a modulator of estradiol leptin induction in placental cells |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01434004_v57_n_p152_Schanton http://hdl.handle.net/20.500.12110/paper_01434004_v57_n_p152_Schanton |
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1768541700375969792 |