Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 μg...

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Autores principales:	Pujol, Carlos Alberto, Carlucci, María Josefina, Damonte, Elsa Beatriz
Publicado:	2008
Materias:	Anti-HSV activity Galactan sulfate Gracilaria corticata antivirus agent galactan Gracilaria corticata extract periodate polysaccharide unclassified drug animal cell anion exchange chromatography anticoagulation antiviral activity article controlled study drug structure drug sulfation gel permeation chromatography Gracilaria Herpes simplex virus 1 Herpes simplex virus 2 IC 50 matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight nonhuman nuclear magnetic resonance spectroscopy Vero cell Animals Anticoagulants Antiviral Agents Cercopithecus aethiops Chromatography, Gel Complex Mixtures Galactans Herpesvirus 1, Human Herpesvirus 2, Human Hydrolysis Magnetic Resonance Spectroscopy Molecular Weight Oxidation-Reduction Periodic Acid Polysaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Vero Cells Simplexvirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v43_n4_p346_Chattopadhyay http://hdl.handle.net/20.500.12110/paper_01418130_v43_n4_p346_Chattopadhyay
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01418130_v43_n4_p346_Chattopadhyay
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spelling	paper:paper_01418130_v43_n4_p346_Chattopadhyay2023-06-08T15:11:24Z Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities Pujol, Carlos Alberto Carlucci, María Josefina Damonte, Elsa Beatriz Anti-HSV activity Galactan sulfate Gracilaria corticata antivirus agent galactan Gracilaria corticata extract periodate polysaccharide unclassified drug animal cell anion exchange chromatography anticoagulation antiviral activity article controlled study drug structure drug sulfation gel permeation chromatography Gracilaria Gracilaria corticata Herpes simplex virus 1 Herpes simplex virus 2 IC 50 matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight nonhuman nuclear magnetic resonance spectroscopy Vero cell Animals Anticoagulants Antiviral Agents Cercopithecus aethiops Chromatography, Gel Complex Mixtures Galactans Gracilaria Herpesvirus 1, Human Herpesvirus 2, Human Hydrolysis Magnetic Resonance Spectroscopy Molecular Weight Oxidation-Reduction Periodic Acid Polysaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Vero Cells Gracilaria corticata Simplexvirus In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 μg/ml. Sub-fraction F3, which has a molecular mass of 30 kDa, consists of a backbone of β-(1 → 3) and α-(1 → 4)-linked-galactopyranosyl residues. This linear galactan contained Gal2Xyl1, Gal2AnGal2, Gal4 and Me-Gal3AnGal2 as oligomeric building subunits. Sulfate group was located at C-4 of (1 → 3)-linked galactopyranosyl residues of the native galactan, and appeared to be very important for the anti-herpetic activity. © 2008 Elsevier B.V. All rights reserved. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v43_n4_p346_Chattopadhyay http://hdl.handle.net/20.500.12110/paper_01418130_v43_n4_p346_Chattopadhyay
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anti-HSV activity Galactan sulfate Gracilaria corticata antivirus agent galactan Gracilaria corticata extract periodate polysaccharide unclassified drug animal cell anion exchange chromatography anticoagulation antiviral activity article controlled study drug structure drug sulfation gel permeation chromatography Gracilaria Gracilaria corticata Herpes simplex virus 1 Herpes simplex virus 2 IC 50 matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight nonhuman nuclear magnetic resonance spectroscopy Vero cell Animals Anticoagulants Antiviral Agents Cercopithecus aethiops Chromatography, Gel Complex Mixtures Galactans Gracilaria Herpesvirus 1, Human Herpesvirus 2, Human Hydrolysis Magnetic Resonance Spectroscopy Molecular Weight Oxidation-Reduction Periodic Acid Polysaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Vero Cells Gracilaria corticata Simplexvirus
spellingShingle	Anti-HSV activity Galactan sulfate Gracilaria corticata antivirus agent galactan Gracilaria corticata extract periodate polysaccharide unclassified drug animal cell anion exchange chromatography anticoagulation antiviral activity article controlled study drug structure drug sulfation gel permeation chromatography Gracilaria Gracilaria corticata Herpes simplex virus 1 Herpes simplex virus 2 IC 50 matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight nonhuman nuclear magnetic resonance spectroscopy Vero cell Animals Anticoagulants Antiviral Agents Cercopithecus aethiops Chromatography, Gel Complex Mixtures Galactans Gracilaria Herpesvirus 1, Human Herpesvirus 2, Human Hydrolysis Magnetic Resonance Spectroscopy Molecular Weight Oxidation-Reduction Periodic Acid Polysaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Vero Cells Gracilaria corticata Simplexvirus Pujol, Carlos Alberto Carlucci, María Josefina Damonte, Elsa Beatriz Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
topic_facet	Anti-HSV activity Galactan sulfate Gracilaria corticata antivirus agent galactan Gracilaria corticata extract periodate polysaccharide unclassified drug animal cell anion exchange chromatography anticoagulation antiviral activity article controlled study drug structure drug sulfation gel permeation chromatography Gracilaria Gracilaria corticata Herpes simplex virus 1 Herpes simplex virus 2 IC 50 matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight nonhuman nuclear magnetic resonance spectroscopy Vero cell Animals Anticoagulants Antiviral Agents Cercopithecus aethiops Chromatography, Gel Complex Mixtures Galactans Gracilaria Herpesvirus 1, Human Herpesvirus 2, Human Hydrolysis Magnetic Resonance Spectroscopy Molecular Weight Oxidation-Reduction Periodic Acid Polysaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Vero Cells Gracilaria corticata Simplexvirus
description	In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 μg/ml. Sub-fraction F3, which has a molecular mass of 30 kDa, consists of a backbone of β-(1 → 3) and α-(1 → 4)-linked-galactopyranosyl residues. This linear galactan contained Gal2Xyl1, Gal2AnGal2, Gal4 and Me-Gal3AnGal2 as oligomeric building subunits. Sulfate group was located at C-4 of (1 → 3)-linked galactopyranosyl residues of the native galactan, and appeared to be very important for the anti-herpetic activity. © 2008 Elsevier B.V. All rights reserved.
author	Pujol, Carlos Alberto Carlucci, María Josefina Damonte, Elsa Beatriz
author_facet	Pujol, Carlos Alberto Carlucci, María Josefina Damonte, Elsa Beatriz
author_sort	Pujol, Carlos Alberto
title	Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
title_short	Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
title_full	Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
title_fullStr	Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
title_full_unstemmed	Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities
title_sort	polysaccharides from gracilaria corticata: sulfation, chemical characterization and anti-hsv activities
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v43_n4_p346_Chattopadhyay http://hdl.handle.net/20.500.12110/paper_01418130_v43_n4_p346_Chattopadhyay
work_keys_str_mv	AT pujolcarlosalberto polysaccharidesfromgracilariacorticatasulfationchemicalcharacterizationandantihsvactivities AT carluccimariajosefina polysaccharidesfromgracilariacorticatasulfationchemicalcharacterizationandantihsvactivities AT damonteelsabeatriz polysaccharidesfromgracilariacorticatasulfationchemicalcharacterizationandantihsvactivities
_version_	1768541603355426816

Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

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