Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites
Enzyme immobilization has attracted great interest in biotechnology processes. Herein we report the immobilization of urease from Canavalia ensiformis (jack bean) in sol-gel-derived silica nanocomposites. Urease activity, differential scanning calorimetry (DSC), nitrogen and water adsorption isother...
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paper:paper_01410229_v42_n7_p583_Desimone2023-06-08T15:11:12Z Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites Matiacevich, Silvia Beatriz Buera, María del Pilar Glycerol Immobilization Nanocomposites Relative humidity Sol-gel Trehalose Urease Adsorption isotherms Biotechnology Differential scanning calorimetry Enzyme activity Nanocomposites Polymers Silica Sol-gels Canavalia ensiformis Organic additives Sol-gel-derived matrices Atmospheric humidity glycerol nanocomposite nitrogen silicon dioxide tetraethoxysilane trehalose urease water adsorption article biocompatibility biosensor biotechnology Canavalia differential scanning calorimetry enzyme activity enzyme immobilization enzyme stability gel humidity jack bean nonhuman storage Canavalia ensiformis Enzyme immobilization has attracted great interest in biotechnology processes. Herein we report the immobilization of urease from Canavalia ensiformis (jack bean) in sol-gel-derived silica nanocomposites. Urease activity, differential scanning calorimetry (DSC), nitrogen and water adsorption isotherms were used to characterize the effect of storage at various relative humidities on enzyme activity immobilized in sol-gel-derived silica nanocomposites. In this study, the nanocomposites consist of tetraethoxysilane, as inorganic silicate precursor, in combination with glycerol or trehalose as organic additives. Entrapped urease was more stable for all the formulations aged with a relative humidity of 80%. However, significant differences (p < 0.05) in enzyme activity recovered at this relative humidity were observed between samples with different formulations, reflecting the effect of additives during the immobilization process. The applications of biocompatible sol-gel-derived matrices can be further extended and utilized in the development of biosensors with immobilized biomolecules that can be used for long time periods by taking into account different factors, among which the storage relative humidity has permitted to greatly improve the stability of the immobilized urease. © 2008 Elsevier Inc. All rights reserved. Fil:Matiacevich, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buera, M.d.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01410229_v42_n7_p583_Desimone http://hdl.handle.net/20.500.12110/paper_01410229_v42_n7_p583_Desimone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glycerol Immobilization Nanocomposites Relative humidity Sol-gel Trehalose Urease Adsorption isotherms Biotechnology Differential scanning calorimetry Enzyme activity Nanocomposites Polymers Silica Sol-gels Canavalia ensiformis Organic additives Sol-gel-derived matrices Atmospheric humidity glycerol nanocomposite nitrogen silicon dioxide tetraethoxysilane trehalose urease water adsorption article biocompatibility biosensor biotechnology Canavalia differential scanning calorimetry enzyme activity enzyme immobilization enzyme stability gel humidity jack bean nonhuman storage Canavalia ensiformis |
| spellingShingle |
Glycerol Immobilization Nanocomposites Relative humidity Sol-gel Trehalose Urease Adsorption isotherms Biotechnology Differential scanning calorimetry Enzyme activity Nanocomposites Polymers Silica Sol-gels Canavalia ensiformis Organic additives Sol-gel-derived matrices Atmospheric humidity glycerol nanocomposite nitrogen silicon dioxide tetraethoxysilane trehalose urease water adsorption article biocompatibility biosensor biotechnology Canavalia differential scanning calorimetry enzyme activity enzyme immobilization enzyme stability gel humidity jack bean nonhuman storage Canavalia ensiformis Matiacevich, Silvia Beatriz Buera, María del Pilar Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| topic_facet |
Glycerol Immobilization Nanocomposites Relative humidity Sol-gel Trehalose Urease Adsorption isotherms Biotechnology Differential scanning calorimetry Enzyme activity Nanocomposites Polymers Silica Sol-gels Canavalia ensiformis Organic additives Sol-gel-derived matrices Atmospheric humidity glycerol nanocomposite nitrogen silicon dioxide tetraethoxysilane trehalose urease water adsorption article biocompatibility biosensor biotechnology Canavalia differential scanning calorimetry enzyme activity enzyme immobilization enzyme stability gel humidity jack bean nonhuman storage Canavalia ensiformis |
| description |
Enzyme immobilization has attracted great interest in biotechnology processes. Herein we report the immobilization of urease from Canavalia ensiformis (jack bean) in sol-gel-derived silica nanocomposites. Urease activity, differential scanning calorimetry (DSC), nitrogen and water adsorption isotherms were used to characterize the effect of storage at various relative humidities on enzyme activity immobilized in sol-gel-derived silica nanocomposites. In this study, the nanocomposites consist of tetraethoxysilane, as inorganic silicate precursor, in combination with glycerol or trehalose as organic additives. Entrapped urease was more stable for all the formulations aged with a relative humidity of 80%. However, significant differences (p < 0.05) in enzyme activity recovered at this relative humidity were observed between samples with different formulations, reflecting the effect of additives during the immobilization process. The applications of biocompatible sol-gel-derived matrices can be further extended and utilized in the development of biosensors with immobilized biomolecules that can be used for long time periods by taking into account different factors, among which the storage relative humidity has permitted to greatly improve the stability of the immobilized urease. © 2008 Elsevier Inc. All rights reserved. |
| author |
Matiacevich, Silvia Beatriz Buera, María del Pilar |
| author_facet |
Matiacevich, Silvia Beatriz Buera, María del Pilar |
| author_sort |
Matiacevich, Silvia Beatriz |
| title |
Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| title_short |
Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| title_full |
Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| title_fullStr |
Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| title_full_unstemmed |
Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| title_sort |
effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01410229_v42_n7_p583_Desimone http://hdl.handle.net/20.500.12110/paper_01410229_v42_n7_p583_Desimone |
| work_keys_str_mv |
AT matiacevichsilviabeatriz effectsofrelativehumidityonenzymeactivityimmobilizedinsolgelderivedsilicananocomposites AT bueramariadelpilar effectsofrelativehumidityonenzymeactivityimmobilizedinsolgelderivedsilicananocomposites |
| _version_ |
1768541745615732736 |