A new world monkey microsatellite (AP74) higly conserved in primates

Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in bloo...

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Detalles Bibliográficos
Autores principales:	Oklander, Luciana Inés, Steinberg, Eliana Ruth, Mudry, Marta Dolores
Publicado:	2012
Materias:	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0120548X_v17_n1_p93_Oklander
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spelling	paper:paper_0120548X_v17_n1_p93_Oklander2023-06-08T15:10:51Z A new world monkey microsatellite (AP74) higly conserved in primates Oklander, Luciana Inés Steinberg, Eliana Ruth Mudry, Marta Dolores Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates. Fil:Oklander, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Steinberg, E.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates
spellingShingle	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates Oklander, Luciana Inés Steinberg, Eliana Ruth Mudry, Marta Dolores A new world monkey microsatellite (AP74) higly conserved in primates
topic_facet	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates
description	Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.
author	Oklander, Luciana Inés Steinberg, Eliana Ruth Mudry, Marta Dolores
author_facet	Oklander, Luciana Inés Steinberg, Eliana Ruth Mudry, Marta Dolores
author_sort	Oklander, Luciana Inés
title	A new world monkey microsatellite (AP74) higly conserved in primates
title_short	A new world monkey microsatellite (AP74) higly conserved in primates
title_full	A new world monkey microsatellite (AP74) higly conserved in primates
title_fullStr	A new world monkey microsatellite (AP74) higly conserved in primates
title_full_unstemmed	A new world monkey microsatellite (AP74) higly conserved in primates
title_sort	new world monkey microsatellite (ap74) higly conserved in primates
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
work_keys_str_mv	AT oklanderlucianaines anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT steinbergelianaruth anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT mudrymartadolores anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT oklanderlucianaines newworldmonkeymicrosatelliteap74higlyconservedinprimates AT steinbergelianaruth newworldmonkeymicrosatelliteap74higlyconservedinprimates AT mudrymartadolores newworldmonkeymicrosatelliteap74higlyconservedinprimates
_version_	1768543173970231296

A new world monkey microsatellite (AP74) higly conserved in primates

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