Design and applications of modified oligonucleotides

Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide anal...

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Publicado:	2003
Materias:	Antisense Aptamers Modified nucleotides Oligonucleotide libraries Ribozymes SELEX method aptamer deoxyribozyme DNA nuclease oligonucleotide ribozyme RNA biotechnology chemical modification combinatorial library gene amplification hybridization molecular biology nucleic acid probe nucleic acid structure nucleotide transport polymerase chain reaction review
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v36_n2_p143_Gallo http://hdl.handle.net/20.500.12110/paper_0100879X_v36_n2_p143_Gallo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0100879X_v36_n2_p143_Gallo
record_format	dspace
spelling	paper:paper_0100879X_v36_n2_p143_Gallo2023-06-08T15:10:16Z Design and applications of modified oligonucleotides Antisense Aptamers Modified nucleotides Oligonucleotide libraries Ribozymes SELEX method aptamer deoxyribozyme DNA nuclease oligonucleotide ribozyme RNA biotechnology chemical modification combinatorial library gene amplification hybridization molecular biology nucleic acid probe nucleic acid structure nucleotide transport polymerase chain reaction review Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly synthesized library on the basis of a particular property. They are identified by repeated cycles of selection and amplification, using PCR technologies. Modified nucleotides can be introduced either during the amplification procedure or after selection. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v36_n2_p143_Gallo http://hdl.handle.net/20.500.12110/paper_0100879X_v36_n2_p143_Gallo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antisense Aptamers Modified nucleotides Oligonucleotide libraries Ribozymes SELEX method aptamer deoxyribozyme DNA nuclease oligonucleotide ribozyme RNA biotechnology chemical modification combinatorial library gene amplification hybridization molecular biology nucleic acid probe nucleic acid structure nucleotide transport polymerase chain reaction review
spellingShingle	Antisense Aptamers Modified nucleotides Oligonucleotide libraries Ribozymes SELEX method aptamer deoxyribozyme DNA nuclease oligonucleotide ribozyme RNA biotechnology chemical modification combinatorial library gene amplification hybridization molecular biology nucleic acid probe nucleic acid structure nucleotide transport polymerase chain reaction review Design and applications of modified oligonucleotides
topic_facet	Antisense Aptamers Modified nucleotides Oligonucleotide libraries Ribozymes SELEX method aptamer deoxyribozyme DNA nuclease oligonucleotide ribozyme RNA biotechnology chemical modification combinatorial library gene amplification hybridization molecular biology nucleic acid probe nucleic acid structure nucleotide transport polymerase chain reaction review
description	Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly synthesized library on the basis of a particular property. They are identified by repeated cycles of selection and amplification, using PCR technologies. Modified nucleotides can be introduced either during the amplification procedure or after selection.
title	Design and applications of modified oligonucleotides
title_short	Design and applications of modified oligonucleotides
title_full	Design and applications of modified oligonucleotides
title_fullStr	Design and applications of modified oligonucleotides
title_full_unstemmed	Design and applications of modified oligonucleotides
title_sort	design and applications of modified oligonucleotides
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v36_n2_p143_Gallo http://hdl.handle.net/20.500.12110/paper_0100879X_v36_n2_p143_Gallo
_version_	1768542261373566976

Design and applications of modified oligonucleotides

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