Bone marrow fibroblasts in patients with advanced lung cancer

In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bon...

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Publicado:	2001
Materias:	Cancer Fibroblasts Interleukin-1 Prostaglandin E2 interleukin 1beta prostaglandin E2 adult advanced cancer article bone marrow cell bone marrow culture cancer patient cell assay cell proliferation clinical article colony forming unit F controlled study culture medium cytokine production enzyme linked immunosorbent assay fibroblast fibroblast culture human human cell lung cancer lung non small cell cancer prostaglandin synthesis radioimmunoassay stroma cell
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v34_n11_p1457_Chasseing http://hdl.handle.net/20.500.12110/paper_0100879X_v34_n11_p1457_Chasseing
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0100879X_v34_n11_p1457_Chasseing
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spelling	paper:paper_0100879X_v34_n11_p1457_Chasseing2023-06-08T15:10:16Z Bone marrow fibroblasts in patients with advanced lung cancer Cancer Fibroblasts Interleukin-1 Prostaglandin E2 interleukin 1beta prostaglandin E2 adult advanced cancer article bone marrow cell bone marrow culture cancer patient cell assay cell proliferation clinical article colony forming unit F controlled study culture medium cytokine production enzyme linked immunosorbent assay fibroblast fibroblast culture human human cell lung cancer lung non small cell cancer prostaglandin synthesis radioimmunoassay stroma cell In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of pure fibroblast cultures were measured with an ELISA kit and RIA kit, respectively. Only fibroblasts from 6/13 (46%) LCP confluent primary cultures had the capacity to proliferate following four subcultures (NC = 100%). Levels of spontaneously released IL-1ß were below 10 pg/ml in the CM of LCP, while NC had a mean value of 1,217 ± 74 pg/ml. In contrast, levels of PGE2 in these CM of LCP were higher (77.5 ± 23.6 pg/ml) compared to NC (18.5 ± 0.9 pg/ml). In conclusion, bone marrow fibroblasts from LCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1ß and PGE2 production. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v34_n11_p1457_Chasseing http://hdl.handle.net/20.500.12110/paper_0100879X_v34_n11_p1457_Chasseing
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cancer Fibroblasts Interleukin-1 Prostaglandin E2 interleukin 1beta prostaglandin E2 adult advanced cancer article bone marrow cell bone marrow culture cancer patient cell assay cell proliferation clinical article colony forming unit F controlled study culture medium cytokine production enzyme linked immunosorbent assay fibroblast fibroblast culture human human cell lung cancer lung non small cell cancer prostaglandin synthesis radioimmunoassay stroma cell
spellingShingle	Cancer Fibroblasts Interleukin-1 Prostaglandin E2 interleukin 1beta prostaglandin E2 adult advanced cancer article bone marrow cell bone marrow culture cancer patient cell assay cell proliferation clinical article colony forming unit F controlled study culture medium cytokine production enzyme linked immunosorbent assay fibroblast fibroblast culture human human cell lung cancer lung non small cell cancer prostaglandin synthesis radioimmunoassay stroma cell Bone marrow fibroblasts in patients with advanced lung cancer
topic_facet	Cancer Fibroblasts Interleukin-1 Prostaglandin E2 interleukin 1beta prostaglandin E2 adult advanced cancer article bone marrow cell bone marrow culture cancer patient cell assay cell proliferation clinical article colony forming unit F controlled study culture medium cytokine production enzyme linked immunosorbent assay fibroblast fibroblast culture human human cell lung cancer lung non small cell cancer prostaglandin synthesis radioimmunoassay stroma cell
description	In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of pure fibroblast cultures were measured with an ELISA kit and RIA kit, respectively. Only fibroblasts from 6/13 (46%) LCP confluent primary cultures had the capacity to proliferate following four subcultures (NC = 100%). Levels of spontaneously released IL-1ß were below 10 pg/ml in the CM of LCP, while NC had a mean value of 1,217 ± 74 pg/ml. In contrast, levels of PGE2 in these CM of LCP were higher (77.5 ± 23.6 pg/ml) compared to NC (18.5 ± 0.9 pg/ml). In conclusion, bone marrow fibroblasts from LCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1ß and PGE2 production.
title	Bone marrow fibroblasts in patients with advanced lung cancer
title_short	Bone marrow fibroblasts in patients with advanced lung cancer
title_full	Bone marrow fibroblasts in patients with advanced lung cancer
title_fullStr	Bone marrow fibroblasts in patients with advanced lung cancer
title_full_unstemmed	Bone marrow fibroblasts in patients with advanced lung cancer
title_sort	bone marrow fibroblasts in patients with advanced lung cancer
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v34_n11_p1457_Chasseing http://hdl.handle.net/20.500.12110/paper_0100879X_v34_n11_p1457_Chasseing
_version_	1768543077532696576

Bone marrow fibroblasts in patients with advanced lung cancer

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