Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli

Manipulation of global regulators is one of the strategies used for the construction of bacterial strains suitable for the synthesis of bioproducts. However, the pleiotropic effects of these regulators can vary under different conditions and are often strain dependent. This study analyzed the effect...

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Guardado en:
Detalles Bibliográficos
Publicado: 2018
Materias:
1,3-propanediol
ArcA
Cra
Escherichia coli
Global regulators
PhaP
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v84_n19_p_Egoburo
http://hdl.handle.net/20.500.12110/paper_00992240_v84_n19_p_Egoburo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00992240_v84_n19_p_Egoburo
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spelling paper:paper_00992240_v84_n19_p_Egoburo2023-06-08T15:10:10Z Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli 1,3-propanediol ArcA Cra Escherichia coli Global regulators PhaP Manipulation of global regulators is one of the strategies used for the construction of bacterial strains suitable for the synthesis of bioproducts. However, the pleiotropic effects of these regulators can vary under different conditions and are often strain dependent. This study analyzed the effects of ArcA, CreC, Cra, and Rob using single deletion mutants of the well-characterized and completely sequenced Escherichia coli strain BW25113. Comparison of the effects of each regulator on the synthesis of major extracellular metabolites, tolerance to several compounds, and synthesis of native and nonnative bioproducts under different growth conditions allowed the discrimination of the particular phenotypes that can be attributed to the individual mutants and singled out Cra and ArcA as the regulators with the most important effects on bacterial metabolism. These data were used to identify the most suitable backgrounds for the synthesis of the reduced bioproducts succinate and 1,3-propanediol (1,3-PDO). The Δcra mutant was further modified to enhance succinate synthesis by the addition of enzymes that increase NADH and CO 2 availability, achieving an 80% increase compared to the parental strain. Production of 1,3-PDO in the ΔarcA mutant was optimized by overexpression of PhaP, which increased more than twice the amount of the diol compared to the wild type in a semidefined medium using glycerol, resulting in 24 g · liter -1 of 1,3-PDO after 48 h, with a volumetric productivity of 0.5 g · liter -1 h -1 . © 2018 American Society for Microbiology. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v84_n19_p_Egoburo http://hdl.handle.net/20.500.12110/paper_00992240_v84_n19_p_Egoburo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 1,3-propanediol
ArcA
Cra
Escherichia coli
Global regulators
PhaP
spellingShingle 1,3-propanediol
ArcA
Cra
Escherichia coli
Global regulators
PhaP
Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
topic_facet 1,3-propanediol
ArcA
Cra
Escherichia coli
Global regulators
PhaP
description Manipulation of global regulators is one of the strategies used for the construction of bacterial strains suitable for the synthesis of bioproducts. However, the pleiotropic effects of these regulators can vary under different conditions and are often strain dependent. This study analyzed the effects of ArcA, CreC, Cra, and Rob using single deletion mutants of the well-characterized and completely sequenced Escherichia coli strain BW25113. Comparison of the effects of each regulator on the synthesis of major extracellular metabolites, tolerance to several compounds, and synthesis of native and nonnative bioproducts under different growth conditions allowed the discrimination of the particular phenotypes that can be attributed to the individual mutants and singled out Cra and ArcA as the regulators with the most important effects on bacterial metabolism. These data were used to identify the most suitable backgrounds for the synthesis of the reduced bioproducts succinate and 1,3-propanediol (1,3-PDO). The Δcra mutant was further modified to enhance succinate synthesis by the addition of enzymes that increase NADH and CO 2 availability, achieving an 80% increase compared to the parental strain. Production of 1,3-PDO in the ΔarcA mutant was optimized by overexpression of PhaP, which increased more than twice the amount of the diol compared to the wild type in a semidefined medium using glycerol, resulting in 24 g · liter -1 of 1,3-PDO after 48 h, with a volumetric productivity of 0.5 g · liter -1 h -1 . © 2018 American Society for Microbiology.
title Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
title_short Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
title_full Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
title_fullStr Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
title_full_unstemmed Microbial cell factories à la carte: Elimination of global regulators Cra and ArcA generates metabolic backgrounds suitable for the synthesis of bioproducts in Escherichia coli
title_sort microbial cell factories à la carte: elimination of global regulators cra and arca generates metabolic backgrounds suitable for the synthesis of bioproducts in escherichia coli
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v84_n19_p_Egoburo
http://hdl.handle.net/20.500.12110/paper_00992240_v84_n19_p_Egoburo
_version_ 1768543077346050048

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