Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence com...
| Autores principales: | , , , |
|---|---|
| Publicado: |
2008
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v74_n24_p7824_Acosta http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta |
| Aporte de: |
| id |
paper:paper_00992240_v74_n24_p7824_Acosta |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00992240_v74_n24_p7824_Acosta2023-06-08T15:10:05Z Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 Prado Acosta, Mariano Palomino, María Mercedes Allievi, Mariana Claudia Ruzal, Sandra Mónica Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity. Copyright © 2008, American Society for Microbiology. All Rights Reserved. Fil:Acosta, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v74_n24_p7824_Acosta http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica |
| spellingShingle |
Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica Prado Acosta, Mariano Palomino, María Mercedes Allievi, Mariana Claudia Ruzal, Sandra Mónica Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| topic_facet |
Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica |
| description |
We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity. Copyright © 2008, American Society for Microbiology. All Rights Reserved. |
| author |
Prado Acosta, Mariano Palomino, María Mercedes Allievi, Mariana Claudia Ruzal, Sandra Mónica |
| author_facet |
Prado Acosta, Mariano Palomino, María Mercedes Allievi, Mariana Claudia Ruzal, Sandra Mónica |
| author_sort |
Prado Acosta, Mariano |
| title |
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| title_short |
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| title_full |
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| title_fullStr |
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| title_full_unstemmed |
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 |
| title_sort |
murein hydrolase activity in the surface layer of lactobacillus acidophilus atcc 4356 |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v74_n24_p7824_Acosta http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta |
| work_keys_str_mv |
AT pradoacostamariano mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT palominomariamercedes mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT allievimarianaclaudia mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT ruzalsandramonica mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 |
| _version_ |
1768542782408884224 |