New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen |
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paper:paper_00992240_v65_n1_p278_Katzen2023-06-08T15:10:02Z New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon Katzen, Federico Ielmini, María Verónica Ielpi, Luis xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan. Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris |
| spellingShingle |
xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris Katzen, Federico Ielmini, María Verónica Ielpi, Luis New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| topic_facet |
xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris |
| description |
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan. |
| author |
Katzen, Federico Ielmini, María Verónica Ielpi, Luis |
| author_facet |
Katzen, Federico Ielmini, María Verónica Ielpi, Luis |
| author_sort |
Katzen, Federico |
| title |
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| title_short |
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| title_full |
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| title_fullStr |
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| title_full_unstemmed |
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon |
| title_sort |
new mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the xanthomonas campestris pv. campestris gum operon |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen |
| work_keys_str_mv |
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| _version_ |
1768542782216994816 |