Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes

In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) pe...

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Autores principales: Denari, Daniela, Ceballos, Nora Raquel
Publicado: 2005
Materias:
11β-hydroxysteroid dehydrogenase
Corticosterone
Testis
Toad
11beta hydroxysteroid dehydrogenase
corticosterone
glucocorticoid
glucose 6 phosphatase
glycyrrhetinic acid
nicotinamide adenine dinucleotide
succinate dehydrogenase
animal experiment
animal tissue
cell nucleus
cellular distribution
conference paper
controlled study
corticosterone blood level
cytosol
enzyme activity
Leydig cell
male
Michaelis constant
microsome
mitochondrion
nonhuman
oxidation
reproduction
seasonal variation
testis
toad
Anura
Bufo arenarum
Mammalia
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00778923_v1040_n_p297_Denari
http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00778923_v1040_n_p297_Denari
record_format dspace
spelling paper:paper_00778923_v1040_n_p297_Denari2023-06-08T15:07:27Z Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes Denari, Daniela Ceballos, Nora Raquel 11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences. Fil:Denari, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00778923_v1040_n_p297_Denari http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 11β-hydroxysteroid dehydrogenase
Corticosterone
Testis
Toad
11beta hydroxysteroid dehydrogenase
corticosterone
glucocorticoid
glucose 6 phosphatase
glycyrrhetinic acid
nicotinamide adenine dinucleotide
succinate dehydrogenase
animal experiment
animal tissue
cell nucleus
cellular distribution
conference paper
controlled study
corticosterone blood level
cytosol
enzyme activity
Leydig cell
male
Michaelis constant
microsome
mitochondrion
nonhuman
oxidation
reproduction
seasonal variation
testis
toad
Anura
Bufo arenarum
Mammalia
spellingShingle 11β-hydroxysteroid dehydrogenase
Corticosterone
Testis
Toad
11beta hydroxysteroid dehydrogenase
corticosterone
glucocorticoid
glucose 6 phosphatase
glycyrrhetinic acid
nicotinamide adenine dinucleotide
succinate dehydrogenase
animal experiment
animal tissue
cell nucleus
cellular distribution
conference paper
controlled study
corticosterone blood level
cytosol
enzyme activity
Leydig cell
male
Michaelis constant
microsome
mitochondrion
nonhuman
oxidation
reproduction
seasonal variation
testis
toad
Anura
Bufo arenarum
Mammalia
Denari, Daniela
Ceballos, Nora Raquel
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
topic_facet 11β-hydroxysteroid dehydrogenase
Corticosterone
Testis
Toad
11beta hydroxysteroid dehydrogenase
corticosterone
glucocorticoid
glucose 6 phosphatase
glycyrrhetinic acid
nicotinamide adenine dinucleotide
succinate dehydrogenase
animal experiment
animal tissue
cell nucleus
cellular distribution
conference paper
controlled study
corticosterone blood level
cytosol
enzyme activity
Leydig cell
male
Michaelis constant
microsome
mitochondrion
nonhuman
oxidation
reproduction
seasonal variation
testis
toad
Anura
Bufo arenarum
Mammalia
description In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences.
author Denari, Daniela
Ceballos, Nora Raquel
author_facet Denari, Daniela
Ceballos, Nora Raquel
author_sort Denari, Daniela
title Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_short Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_full Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_fullStr Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_full_unstemmed Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_sort seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
publishDate 2005
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00778923_v1040_n_p297_Denari
http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
work_keys_str_mv AT denaridaniela seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes
AT ceballosnoraraquel seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes
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