Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina

We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection...

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Autores principales:	Suarez, Olga Virginia, Cueto, Gerardo Ruben, Cavia, Regino, Bilenca, David Norberto, Edelstein, Alexis, Hodara, Karina, Padula, Paula Julieta, Busch, Maria
Publicado:	2003
Materias:	Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection article enzyme linked immunosorbent assay gene sequence genotype Hantavirus histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v98_n6_p727_Suarez http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00740276_v98_n6_p727_Suarez2025-07-30T17:47:15Z Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina Suarez, Olga Virginia Cueto, Gerardo Ruben Cavia, Regino Bilenca, David Norberto Edelstein, Alexis Hodara, Karina Padula, Paula Julieta Busch, Maria Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today. Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v98_n6_p727_Suarez http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia
spellingShingle	Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia Suarez, Olga Virginia Cueto, Gerardo Ruben Cavia, Regino Bilenca, David Norberto Edelstein, Alexis Hodara, Karina Padula, Paula Julieta Busch, Maria Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
topic_facet	Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia
description	We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.
author	Suarez, Olga Virginia Cueto, Gerardo Ruben Cavia, Regino Bilenca, David Norberto Edelstein, Alexis Hodara, Karina Padula, Paula Julieta Busch, Maria
author_facet	Suarez, Olga Virginia Cueto, Gerardo Ruben Cavia, Regino Bilenca, David Norberto Edelstein, Alexis Hodara, Karina Padula, Paula Julieta Busch, Maria
author_sort	Suarez, Olga Virginia
title	Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_short	Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_full	Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_fullStr	Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_full_unstemmed	Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_sort	prevalence of infection with hantavirus in rodent populations of central argentina
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v98_n6_p727_Suarez http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez
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Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina

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