Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food

The stimulatory effect of the spiperone and naloxone on the ovarian growth was evaluated in females of Aegla uruguayana Schmitt, 1942, being that these neuroregulators were incorporated to food and administrated at a dose of 10-8 mol/animal to each session food. Adult females were sampled with nets...

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Detalles Bibliográficos
Autores principales: Cahansky, Alejandra Valeria, Rodriguez, Enrique Marcelo
Publicado: 2009
Materias:
Anomura
Intermediary metabolism
Naloxone
Ovarian growth
Spiperona
Aegla uruguayana
Aeglidae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00734721_v99_n3_p286_Castiglioni
http://hdl.handle.net/20.500.12110/paper_00734721_v99_n3_p286_Castiglioni
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00734721_v99_n3_p286_Castiglioni
record_format dspace
spelling paper:paper_00734721_v99_n3_p286_Castiglioni2023-06-08T15:06:43Z Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food Cahansky, Alejandra Valeria Rodriguez, Enrique Marcelo Anomura Intermediary metabolism Naloxone Ovarian growth Spiperona Aegla uruguayana Aeglidae Anomura The stimulatory effect of the spiperone and naloxone on the ovarian growth was evaluated in females of Aegla uruguayana Schmitt, 1942, being that these neuroregulators were incorporated to food and administrated at a dose of 10-8 mol/animal to each session food. Adult females were sampled with nets in a stream near the municipality of Salto, Province of Buenos Aires, Argentina. At the beginning of the experiment, 10 females were randomly selected, sacrificed, weighed and their ovaries were quickly dissected and weighed to serve as the initial control for evaluating the degree of ovarian growth (gonadosomatic index -GI). Others females (30) were divided in three experimental groups - (a) control: females fed on control pellets composed by fish food - 34% protein and 43% protein; (b) spiperone: females fed on pellets enriched with the dopaminegic antagonist spiperone; (c) naloxone: females fed on pellets enriched with the enkephalinergic antagonist naloxone. After seven week of experiment the females were sacrificed and evaluated the GI. The lipids and cholesterol levels of ovaries and hepatopancreas were quantified. Naloxone produced a significant increase of lipids levels in both ovaries and hepatopancreas in relation to control group. Spiperone caused significant increase of lipids levels at the gonads and hepatopancreas and cholesterol in hepatopancreas when compared with the control. The lipids levels were significantly lower in hemolymph of the females that were fed with pellets with spiperone and higher at the females treated with naloxone when compared to females that were fed only fish food. The spiperone and naloxone when inhibited the effect of the dopamine and endogenous opioids, probably caused the secretion of the gonad stimulating hormone and the inhibition of the gonad inhibiting hormone, therefore induction of the ovarian development. Such hypothesis can be strengthened for the increases of gonadosomatic indices in these experimental groups. Fil:Cahansky, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00734721_v99_n3_p286_Castiglioni http://hdl.handle.net/20.500.12110/paper_00734721_v99_n3_p286_Castiglioni
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Anomura
Intermediary metabolism
Naloxone
Ovarian growth
Spiperona
Aegla uruguayana
Aeglidae
Anomura
spellingShingle Anomura
Intermediary metabolism
Naloxone
Ovarian growth
Spiperona
Aegla uruguayana
Aeglidae
Anomura
Cahansky, Alejandra Valeria
Rodriguez, Enrique Marcelo
Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
topic_facet Anomura
Intermediary metabolism
Naloxone
Ovarian growth
Spiperona
Aegla uruguayana
Aeglidae
Anomura
description The stimulatory effect of the spiperone and naloxone on the ovarian growth was evaluated in females of Aegla uruguayana Schmitt, 1942, being that these neuroregulators were incorporated to food and administrated at a dose of 10-8 mol/animal to each session food. Adult females were sampled with nets in a stream near the municipality of Salto, Province of Buenos Aires, Argentina. At the beginning of the experiment, 10 females were randomly selected, sacrificed, weighed and their ovaries were quickly dissected and weighed to serve as the initial control for evaluating the degree of ovarian growth (gonadosomatic index -GI). Others females (30) were divided in three experimental groups - (a) control: females fed on control pellets composed by fish food - 34% protein and 43% protein; (b) spiperone: females fed on pellets enriched with the dopaminegic antagonist spiperone; (c) naloxone: females fed on pellets enriched with the enkephalinergic antagonist naloxone. After seven week of experiment the females were sacrificed and evaluated the GI. The lipids and cholesterol levels of ovaries and hepatopancreas were quantified. Naloxone produced a significant increase of lipids levels in both ovaries and hepatopancreas in relation to control group. Spiperone caused significant increase of lipids levels at the gonads and hepatopancreas and cholesterol in hepatopancreas when compared with the control. The lipids levels were significantly lower in hemolymph of the females that were fed with pellets with spiperone and higher at the females treated with naloxone when compared to females that were fed only fish food. The spiperone and naloxone when inhibited the effect of the dopamine and endogenous opioids, probably caused the secretion of the gonad stimulating hormone and the inhibition of the gonad inhibiting hormone, therefore induction of the ovarian development. Such hypothesis can be strengthened for the increases of gonadosomatic indices in these experimental groups.
author Cahansky, Alejandra Valeria
Rodriguez, Enrique Marcelo
author_facet Cahansky, Alejandra Valeria
Rodriguez, Enrique Marcelo
author_sort Cahansky, Alejandra Valeria
title Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
title_short Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
title_full Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
title_fullStr Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
title_full_unstemmed Induction of ovarian growth in Aegla uruguayana (Anomura, Aeglidae) by means of neuroregulators incorporated to food
title_sort induction of ovarian growth in aegla uruguayana (anomura, aeglidae) by means of neuroregulators incorporated to food
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00734721_v99_n3_p286_Castiglioni
http://hdl.handle.net/20.500.12110/paper_00734721_v99_n3_p286_Castiglioni
work_keys_str_mv AT cahanskyalejandravaleria inductionofovariangrowthinaeglauruguayanaanomuraaeglidaebymeansofneuroregulatorsincorporatedtofood
AT rodriguezenriquemarcelo inductionofovariangrowthinaeglauruguayanaanomuraaeglidaebymeansofneuroregulatorsincorporatedtofood
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