Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus
Azinphos-methyl is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. The oligochaete Lumbriculus variegatus and pigmented and non-pigmented specimens of the gastropod Biomphalaria glabrata are freshwater invertebrates that have been recommende...
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| Publicado: |
2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v72_n9_p1333_Kristoff http://hdl.handle.net/20.500.12110/paper_00456535_v72_n9_p1333_Kristoff |
| Aporte de: |
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paper:paper_00456535_v72_n9_p1333_Kristoff |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Catalase Cholinesterase Freshwater invertebrate Glutathione Organophosphate Superoxide dismutase Amperometric sensors Canning Enzymes Food preservation Glucose Insect control Microfluidics Occupational diseases Oxygen Pest control Photoacoustic effect Plants (botany) System theory (abiotic and biotic) stress Antioxidant defenses Antioxidant responses Azinphos methyl (AZP) Biphasic response Catalase (CAT) Cholinesterase (ChE) Elsevier (CO) food crops Freshwater invertebrates Glucose-6-phosphate dehydrogenase (G6PDH) Glutathione (GSH) Glutathione-S-transferase (GST) In-vivo Neurotoxic effects Non-enzymatic SOD activity soft tissues Superoxide dismutase (SOD1) Whole body (WB) Agricultural engineering azinphos methyl catalase cholinesterase glucose 6 phosphate dehydrogenase glutathione glutathione transferase organophosphate superoxide dismutase annelid antioxidant defense mechanism enzyme activity gastropod insecticide organophosphate pollution effect pollution exposure animal tissue annelid worm antioxidant activity article Biomphalaria glabrata controlled study enzyme activity gastropod Lumbriculus variegatus neurotoxicity nonhuman oxidative stress phenotype soft tissue Animals Antioxidants Azinphosmethyl Biomphalaria Catalase Cholinesterases Enzymes Glutathione Glyceraldehyde-3-Phosphate Dehydrogenases Insecticides Oligochaeta Phenotype Proteins Superoxide Dismutase Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) |
| spellingShingle |
Catalase Cholinesterase Freshwater invertebrate Glutathione Organophosphate Superoxide dismutase Amperometric sensors Canning Enzymes Food preservation Glucose Insect control Microfluidics Occupational diseases Oxygen Pest control Photoacoustic effect Plants (botany) System theory (abiotic and biotic) stress Antioxidant defenses Antioxidant responses Azinphos methyl (AZP) Biphasic response Catalase (CAT) Cholinesterase (ChE) Elsevier (CO) food crops Freshwater invertebrates Glucose-6-phosphate dehydrogenase (G6PDH) Glutathione (GSH) Glutathione-S-transferase (GST) In-vivo Neurotoxic effects Non-enzymatic SOD activity soft tissues Superoxide dismutase (SOD1) Whole body (WB) Agricultural engineering azinphos methyl catalase cholinesterase glucose 6 phosphate dehydrogenase glutathione glutathione transferase organophosphate superoxide dismutase annelid antioxidant defense mechanism enzyme activity gastropod insecticide organophosphate pollution effect pollution exposure animal tissue annelid worm antioxidant activity article Biomphalaria glabrata controlled study enzyme activity gastropod Lumbriculus variegatus neurotoxicity nonhuman oxidative stress phenotype soft tissue Animals Antioxidants Azinphosmethyl Biomphalaria Catalase Cholinesterases Enzymes Glutathione Glyceraldehyde-3-Phosphate Dehydrogenases Insecticides Oligochaeta Phenotype Proteins Superoxide Dismutase Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| topic_facet |
Catalase Cholinesterase Freshwater invertebrate Glutathione Organophosphate Superoxide dismutase Amperometric sensors Canning Enzymes Food preservation Glucose Insect control Microfluidics Occupational diseases Oxygen Pest control Photoacoustic effect Plants (botany) System theory (abiotic and biotic) stress Antioxidant defenses Antioxidant responses Azinphos methyl (AZP) Biphasic response Catalase (CAT) Cholinesterase (ChE) Elsevier (CO) food crops Freshwater invertebrates Glucose-6-phosphate dehydrogenase (G6PDH) Glutathione (GSH) Glutathione-S-transferase (GST) In-vivo Neurotoxic effects Non-enzymatic SOD activity soft tissues Superoxide dismutase (SOD1) Whole body (WB) Agricultural engineering azinphos methyl catalase cholinesterase glucose 6 phosphate dehydrogenase glutathione glutathione transferase organophosphate superoxide dismutase annelid antioxidant defense mechanism enzyme activity gastropod insecticide organophosphate pollution effect pollution exposure animal tissue annelid worm antioxidant activity article Biomphalaria glabrata controlled study enzyme activity gastropod Lumbriculus variegatus neurotoxicity nonhuman oxidative stress phenotype soft tissue Animals Antioxidants Azinphosmethyl Biomphalaria Catalase Cholinesterases Enzymes Glutathione Glyceraldehyde-3-Phosphate Dehydrogenases Insecticides Oligochaeta Phenotype Proteins Superoxide Dismutase Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) |
| description |
Azinphos-methyl is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. The oligochaete Lumbriculus variegatus and pigmented and non-pigmented specimens of the gastropod Biomphalaria glabrata are freshwater invertebrates that have been recommended for contamination studies. Recently, it has been shown that L. variegatus worms exhibit a higher cholinesterase (ChE) activity and a greater sensitivity to in vivo ChE inhibition by azinphos-methyl than pigmented B. glabrata snails. The aims of the present study were (1) to investigate if, in addition to its anticholinesterase action, azinphos-methyl has also pro-oxidant activity in L. variegatus and B. glabrata, and (2) to examine if species that are highly susceptible to the neurotoxic effects of organophosphates also suffer a greater degree of oxidative stress. Therefore, total glutathione (t-GSH) levels and activities of cholinesterase (ChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glucose 6-phosphate dehydrogenase (G6PDH) were measured in the whole body soft tissue of organisms exposed for 48 and 96 h to a level of azinphos-methyl that produces 50% of inhibition on ChE. Results showed different patterns of antioxidant responses between the gastropods and the oligochaetes, and even between the two phenotypes of gastropods: (1) in exposed L. variegatus t-GSH levels increased and CAT and SOD activities decreased with respect to control organisms, (2) in pigmented gastropods, SOD decreased while CAT transiently diminished, and (3) in non-pigmented gastropods, SOD activity showed a biphasic response. GST and G6PDH were not altered by azinphos-methyl exposure. Of note, t-GSH levels were 4-fold times higher in L. variegatus than in both phenotypes of B. glabrata. This may suggest that GSH could play a more important role in antioxidant defense in L. variegatus than in B. glabrata. © 2008 Elsevier Ltd. All rights reserved. |
| author |
Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina |
| author_facet |
Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina |
| author_sort |
Kristoff, Gisela |
| title |
Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| title_short |
Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| title_full |
Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| title_fullStr |
Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| title_full_unstemmed |
Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus |
| title_sort |
effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in biomphalaria glabrata and lumbriculus variegatus |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v72_n9_p1333_Kristoff http://hdl.handle.net/20.500.12110/paper_00456535_v72_n9_p1333_Kristoff |
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| _version_ |
1768541601074774016 |
| spelling |
paper:paper_00456535_v72_n9_p1333_Kristoff2023-06-08T15:05:19Z Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Catalase Cholinesterase Freshwater invertebrate Glutathione Organophosphate Superoxide dismutase Amperometric sensors Canning Enzymes Food preservation Glucose Insect control Microfluidics Occupational diseases Oxygen Pest control Photoacoustic effect Plants (botany) System theory (abiotic and biotic) stress Antioxidant defenses Antioxidant responses Azinphos methyl (AZP) Biphasic response Catalase (CAT) Cholinesterase (ChE) Elsevier (CO) food crops Freshwater invertebrates Glucose-6-phosphate dehydrogenase (G6PDH) Glutathione (GSH) Glutathione-S-transferase (GST) In-vivo Neurotoxic effects Non-enzymatic SOD activity soft tissues Superoxide dismutase (SOD1) Whole body (WB) Agricultural engineering azinphos methyl catalase cholinesterase glucose 6 phosphate dehydrogenase glutathione glutathione transferase organophosphate superoxide dismutase annelid antioxidant defense mechanism enzyme activity gastropod insecticide organophosphate pollution effect pollution exposure animal tissue annelid worm antioxidant activity article Biomphalaria glabrata controlled study enzyme activity gastropod Lumbriculus variegatus neurotoxicity nonhuman oxidative stress phenotype soft tissue Animals Antioxidants Azinphosmethyl Biomphalaria Catalase Cholinesterases Enzymes Glutathione Glyceraldehyde-3-Phosphate Dehydrogenases Insecticides Oligochaeta Phenotype Proteins Superoxide Dismutase Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) Azinphos-methyl is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. The oligochaete Lumbriculus variegatus and pigmented and non-pigmented specimens of the gastropod Biomphalaria glabrata are freshwater invertebrates that have been recommended for contamination studies. Recently, it has been shown that L. variegatus worms exhibit a higher cholinesterase (ChE) activity and a greater sensitivity to in vivo ChE inhibition by azinphos-methyl than pigmented B. glabrata snails. The aims of the present study were (1) to investigate if, in addition to its anticholinesterase action, azinphos-methyl has also pro-oxidant activity in L. variegatus and B. glabrata, and (2) to examine if species that are highly susceptible to the neurotoxic effects of organophosphates also suffer a greater degree of oxidative stress. Therefore, total glutathione (t-GSH) levels and activities of cholinesterase (ChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glucose 6-phosphate dehydrogenase (G6PDH) were measured in the whole body soft tissue of organisms exposed for 48 and 96 h to a level of azinphos-methyl that produces 50% of inhibition on ChE. Results showed different patterns of antioxidant responses between the gastropods and the oligochaetes, and even between the two phenotypes of gastropods: (1) in exposed L. variegatus t-GSH levels increased and CAT and SOD activities decreased with respect to control organisms, (2) in pigmented gastropods, SOD decreased while CAT transiently diminished, and (3) in non-pigmented gastropods, SOD activity showed a biphasic response. GST and G6PDH were not altered by azinphos-methyl exposure. Of note, t-GSH levels were 4-fold times higher in L. variegatus than in both phenotypes of B. glabrata. This may suggest that GSH could play a more important role in antioxidant defense in L. variegatus than in B. glabrata. © 2008 Elsevier Ltd. All rights reserved. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Verrengia Guerrero, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v72_n9_p1333_Kristoff http://hdl.handle.net/20.500.12110/paper_00456535_v72_n9_p1333_Kristoff |