Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus
Neuropeptide Y (NPY) and orexin are neuropeptides involved in the regulation of feeding in vertebrates. In this study we determined the NPY and orexin mRNA tissue expression and their immunoreactivity distribution in both preoptic area and hypothalamus, regions involved in the regulation of feeding...
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2013
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paper:paper_00408166_v45_n6_p452_PerezSirkin2023-06-08T15:04:42Z Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus Cánepa, Maximiliano Martín Vissio, Paula Gabriela Brain Fish NPY Orexin Pituitary messenger RNA neuropeptide Y orexin adenohypophysis animal cell animal tissue article behavior control brain tissue cellular distribution Cichlasoma dimerus cichlid controlled study endocrine function feeding behavior female fish hypothalamus hypothalamus periventricular nucleus immunocompetent cell immunohistochemistry immunoreactivity male nerve fiber neurohypophysis nonhuman nucleotide sequence preoptic area protein analysis protein expression protein function protein localization thalamus ventral nucleus tuberal nucleus Cichlasoma Cichlidae Vertebrata Brain Fish NPY Orexin Pituitary Animals Cichlids Hypothalamus Intracellular Signaling Peptides and Proteins Neuropeptide Y Neuropeptides Preoptic Area Neuropeptide Y (NPY) and orexin are neuropeptides involved in the regulation of feeding in vertebrates. In this study we determined the NPY and orexin mRNA tissue expression and their immunoreactivity distribution in both preoptic area and hypothalamus, regions involved in the regulation of feeding behavior. Both peptides presented a wide expression in all tissues examined. The NPY-immunoreactive (ir) cells were localized in the ventral nucleus posterioris periventricularis (NPPv) and numerous ir-NPY fibers were found in the nucleus lateralis tuberis (NLT), the nucleus recess lateralis (NRL) and the neurohypophysis. Ir-orexin cells were observed in the NPPv, dorsal NLT, ventral NLT, lateral NLT (NLTl) and the lateral NRL. Ir-orexin fibers were widespread distributed along all the hypothalamus, especially in the NLTl. Additionally, we observed the presence of ir-orexin immunostaining in adenohypophyseal cells, especially in somatotroph cells and the presence of a few ir-orexin-A fibers in the neurohypophysis. In conclusion, both peptides have an ubiquitous mRNA tissue expression and are similarly distributed in the hypothalamus and preoptic area of Cichlasoma dimerus. The presence of ir-orexin in adenohypohyseal cells and the presence of ir-orexin and NPY fibers in the neurohypophysis suggest that both peptides may play an important neuroendocrine role in anterior pituitary. © 2013 Elsevier Ltd. Fil:Cánepa, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v45_n6_p452_PerezSirkin http://hdl.handle.net/20.500.12110/paper_00408166_v45_n6_p452_PerezSirkin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brain Fish NPY Orexin Pituitary messenger RNA neuropeptide Y orexin adenohypophysis animal cell animal tissue article behavior control brain tissue cellular distribution Cichlasoma dimerus cichlid controlled study endocrine function feeding behavior female fish hypothalamus hypothalamus periventricular nucleus immunocompetent cell immunohistochemistry immunoreactivity male nerve fiber neurohypophysis nonhuman nucleotide sequence preoptic area protein analysis protein expression protein function protein localization thalamus ventral nucleus tuberal nucleus Cichlasoma Cichlidae Vertebrata Brain Fish NPY Orexin Pituitary Animals Cichlids Hypothalamus Intracellular Signaling Peptides and Proteins Neuropeptide Y Neuropeptides Preoptic Area |
| spellingShingle |
Brain Fish NPY Orexin Pituitary messenger RNA neuropeptide Y orexin adenohypophysis animal cell animal tissue article behavior control brain tissue cellular distribution Cichlasoma dimerus cichlid controlled study endocrine function feeding behavior female fish hypothalamus hypothalamus periventricular nucleus immunocompetent cell immunohistochemistry immunoreactivity male nerve fiber neurohypophysis nonhuman nucleotide sequence preoptic area protein analysis protein expression protein function protein localization thalamus ventral nucleus tuberal nucleus Cichlasoma Cichlidae Vertebrata Brain Fish NPY Orexin Pituitary Animals Cichlids Hypothalamus Intracellular Signaling Peptides and Proteins Neuropeptide Y Neuropeptides Preoptic Area Cánepa, Maximiliano Martín Vissio, Paula Gabriela Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| topic_facet |
Brain Fish NPY Orexin Pituitary messenger RNA neuropeptide Y orexin adenohypophysis animal cell animal tissue article behavior control brain tissue cellular distribution Cichlasoma dimerus cichlid controlled study endocrine function feeding behavior female fish hypothalamus hypothalamus periventricular nucleus immunocompetent cell immunohistochemistry immunoreactivity male nerve fiber neurohypophysis nonhuman nucleotide sequence preoptic area protein analysis protein expression protein function protein localization thalamus ventral nucleus tuberal nucleus Cichlasoma Cichlidae Vertebrata Brain Fish NPY Orexin Pituitary Animals Cichlids Hypothalamus Intracellular Signaling Peptides and Proteins Neuropeptide Y Neuropeptides Preoptic Area |
| description |
Neuropeptide Y (NPY) and orexin are neuropeptides involved in the regulation of feeding in vertebrates. In this study we determined the NPY and orexin mRNA tissue expression and their immunoreactivity distribution in both preoptic area and hypothalamus, regions involved in the regulation of feeding behavior. Both peptides presented a wide expression in all tissues examined. The NPY-immunoreactive (ir) cells were localized in the ventral nucleus posterioris periventricularis (NPPv) and numerous ir-NPY fibers were found in the nucleus lateralis tuberis (NLT), the nucleus recess lateralis (NRL) and the neurohypophysis. Ir-orexin cells were observed in the NPPv, dorsal NLT, ventral NLT, lateral NLT (NLTl) and the lateral NRL. Ir-orexin fibers were widespread distributed along all the hypothalamus, especially in the NLTl. Additionally, we observed the presence of ir-orexin immunostaining in adenohypophyseal cells, especially in somatotroph cells and the presence of a few ir-orexin-A fibers in the neurohypophysis. In conclusion, both peptides have an ubiquitous mRNA tissue expression and are similarly distributed in the hypothalamus and preoptic area of Cichlasoma dimerus. The presence of ir-orexin in adenohypohyseal cells and the presence of ir-orexin and NPY fibers in the neurohypophysis suggest that both peptides may play an important neuroendocrine role in anterior pituitary. © 2013 Elsevier Ltd. |
| author |
Cánepa, Maximiliano Martín Vissio, Paula Gabriela |
| author_facet |
Cánepa, Maximiliano Martín Vissio, Paula Gabriela |
| author_sort |
Cánepa, Maximiliano Martín |
| title |
Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| title_short |
Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| title_full |
Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| title_fullStr |
Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| title_full_unstemmed |
Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus |
| title_sort |
orexin and neuropeptide y: tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish cichlasoma dimerus |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v45_n6_p452_PerezSirkin http://hdl.handle.net/20.500.12110/paper_00408166_v45_n6_p452_PerezSirkin |
| work_keys_str_mv |
AT canepamaximilianomartin orexinandneuropeptideytissuespecificexpressionandimmunoreactivityinthehypothalamusandpreopticareaofthecichlidfishcichlasomadimerus AT vissiopaulagabriela orexinandneuropeptideytissuespecificexpressionandimmunoreactivityinthehypothalamusandpreopticareaofthecichlidfishcichlasomadimerus |
| _version_ |
1768545179317305344 |