Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS

Key message: Fine mapping permits the precise positioning of genes within chromosomes, prerequisite for positional cloning that will allow its rational use and the study of the underlying molecular action mechanism. Abstract: Three leaf rust resistance genes were identified in the durable leaf rust...

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Autores principales: Dieguez, María José, Velasquez, Silvia Melina, Ingala, Lorena Romina
Publicado: 2014
Materias:
Chromosomes
Cloning
DNA sequences
Genes
Mapping
Plants (botany)
Action mechanisms
Adult plant resistances
Leaf rust resistance genes
Microsatellite markers
Positional cloning
Precise positioning
Seedling resistance
Wheat chromosomes
Genetic engineering
genetic marker
Basidiomycetes
chromosome map
disease resistance
genetics
microbiology
plant chromosome
plant disease
plant gene
wheat
Basidiomycota
Chromosome Mapping
Chromosomes, Plant
Disease Resistance
Genes, Plant
Genetic Markers
Plant Diseases
Triticum
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00405752_v127_n5_p1133_Dieguez
http://hdl.handle.net/20.500.12110/paper_00405752_v127_n5_p1133_Dieguez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00405752_v127_n5_p1133_Dieguez
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spelling paper:paper_00405752_v127_n5_p1133_Dieguez2025-07-30T17:45:05Z Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS Dieguez, María José Velasquez, Silvia Melina Ingala, Lorena Romina Chromosomes Cloning DNA sequences Genes Mapping Plants (botany) Action mechanisms Adult plant resistances Leaf rust resistance genes Microsatellite markers Positional cloning Precise positioning Seedling resistance Wheat chromosomes Genetic engineering genetic marker Basidiomycetes chromosome map disease resistance genetic marker genetics microbiology plant chromosome plant disease plant gene wheat Basidiomycota Chromosome Mapping Chromosomes, Plant Disease Resistance Genes, Plant Genetic Markers Plant Diseases Triticum Key message: Fine mapping permits the precise positioning of genes within chromosomes, prerequisite for positional cloning that will allow its rational use and the study of the underlying molecular action mechanism. Abstract: Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. © 2014, Springer-Verlag Berlin Heidelberg. Fil:Diéguez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Velasquez, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ingala, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00405752_v127_n5_p1133_Dieguez http://hdl.handle.net/20.500.12110/paper_00405752_v127_n5_p1133_Dieguez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chromosomes
Cloning
DNA sequences
Genes
Mapping
Plants (botany)
Action mechanisms
Adult plant resistances
Leaf rust resistance genes
Microsatellite markers
Positional cloning
Precise positioning
Seedling resistance
Wheat chromosomes
Genetic engineering
genetic marker
Basidiomycetes
chromosome map
disease resistance
genetic marker
genetics
microbiology
plant chromosome
plant disease
plant gene
wheat
Basidiomycota
Chromosome Mapping
Chromosomes, Plant
Disease Resistance
Genes, Plant
Genetic Markers
Plant Diseases
Triticum
spellingShingle Chromosomes
Cloning
DNA sequences
Genes
Mapping
Plants (botany)
Action mechanisms
Adult plant resistances
Leaf rust resistance genes
Microsatellite markers
Positional cloning
Precise positioning
Seedling resistance
Wheat chromosomes
Genetic engineering
genetic marker
Basidiomycetes
chromosome map
disease resistance
genetic marker
genetics
microbiology
plant chromosome
plant disease
plant gene
wheat
Basidiomycota
Chromosome Mapping
Chromosomes, Plant
Disease Resistance
Genes, Plant
Genetic Markers
Plant Diseases
Triticum
Dieguez, María José
Velasquez, Silvia Melina
Ingala, Lorena Romina
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
topic_facet Chromosomes
Cloning
DNA sequences
Genes
Mapping
Plants (botany)
Action mechanisms
Adult plant resistances
Leaf rust resistance genes
Microsatellite markers
Positional cloning
Precise positioning
Seedling resistance
Wheat chromosomes
Genetic engineering
genetic marker
Basidiomycetes
chromosome map
disease resistance
genetic marker
genetics
microbiology
plant chromosome
plant disease
plant gene
wheat
Basidiomycota
Chromosome Mapping
Chromosomes, Plant
Disease Resistance
Genes, Plant
Genetic Markers
Plant Diseases
Triticum
description Key message: Fine mapping permits the precise positioning of genes within chromosomes, prerequisite for positional cloning that will allow its rational use and the study of the underlying molecular action mechanism. Abstract: Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. © 2014, Springer-Verlag Berlin Heidelberg.
author Dieguez, María José
Velasquez, Silvia Melina
Ingala, Lorena Romina
author_facet Dieguez, María José
Velasquez, Silvia Melina
Ingala, Lorena Romina
author_sort Dieguez, María José
title Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_short Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_full Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_fullStr Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_full_unstemmed Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_sort fine mapping of lrsv2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3bs
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00405752_v127_n5_p1133_Dieguez
http://hdl.handle.net/20.500.12110/paper_00405752_v127_n5_p1133_Dieguez
work_keys_str_mv AT dieguezmariajose finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs
AT velasquezsilviamelina finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs
AT ingalalorenaromina finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs
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