Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay

Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay...

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Autores principales:	Bonetto, Marìa Celina, Sacco, Natalia Jimena, Cortón, Eduardo
Publicado:	2011
Materias:	Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Oxygen Amperometric detection Environmental strain Long-term incubation Municipal wastewaters
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v85_n1_p455_Bonetto http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00399140_v85_n1_p455_Bonetto
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spelling	paper:paper_00399140_v85_n1_p455_Bonetto2025-07-30T17:44:20Z Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay Bonetto, Marìa Celina Sacco, Natalia Jimena Cortón, Eduardo Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved. Fil:Bonetto, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sacco, N.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v85_n1_p455_Bonetto http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay
spellingShingle	Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay Bonetto, Marìa Celina Sacco, Natalia Jimena Cortón, Eduardo Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
topic_facet	Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay
description	Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved.
author	Bonetto, Marìa Celina Sacco, Natalia Jimena Cortón, Eduardo
author_facet	Bonetto, Marìa Celina Sacco, Natalia Jimena Cortón, Eduardo
author_sort	Bonetto, Marìa Celina
title	Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
title_short	Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
title_full	Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
title_fullStr	Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
title_full_unstemmed	Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
title_sort	assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated bod assay
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v85_n1_p455_Bonetto http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto
work_keys_str_mv	AT bonettomariacelina assessingtheeffectofoxygenandmicrobialinhibitorstooptimizeferricyanidemediatedbodassay AT sacconataliajimena assessingtheeffectofoxygenandmicrobialinhibitorstooptimizeferricyanidemediatedbodassay AT cortoneduardo assessingtheeffectofoxygenandmicrobialinhibitorstooptimizeferricyanidemediatedbodassay
_version_	1840325237041790976

Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay

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