Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay
From the organic extracts of the sponge Siphonochalina fortis, collected at Bahia Bustamante, Chubut, Argentina, three major compounds were isolated and identified as deoxycholic acid 3, 12-diacetate (1), cholic acid 3, 7, 12-triacetate (2) and cholic acid, 3, 7, 12-triacetate. (3). This is the firs...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v78_n10_p982_Cano http://hdl.handle.net/20.500.12110/paper_0039128X_v78_n10_p982_Cano |
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paper:paper_0039128X_v78_n10_p982_Cano2023-06-08T15:03:21Z Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges bile acid cholic acid 3,7 diacetate cholic acid 3,7,12 triacetate deoxycholic acid 3,12 diacetate unclassified drug acetylation article cell viability comet assay controlled study detoxification DNA damage in vitro study metabolite nonhuman peripheral lymphocyte Siphonochalina fortis sponge (Porifera) Bacteria (microorganisms) Siphonochalina Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges Animals Cell Survival Cells, Cultured Cholic Acids Comet Assay DNA Damage Drug Evaluation, Preclinical Humans Lymphocytes Mutagens Porifera From the organic extracts of the sponge Siphonochalina fortis, collected at Bahia Bustamante, Chubut, Argentina, three major compounds were isolated and identified as deoxycholic acid 3, 12-diacetate (1), cholic acid 3, 7, 12-triacetate (2) and cholic acid, 3, 7, 12-triacetate. (3). This is the first report of acetylated bile acids in sponges and the first isolation of compound 3 as a natural product. The potential induction of DNA lesions by the isolated compounds was investigated using the comet assay in lymphocytes of human peripheral blood as in vitro model. The results showed that the administration of the bile acid derivatives would not induce DNA damages, indicating that acetylated bile acids are nontoxic metabolites at the tested concentrations. Since the free bile acids were not detected, it is unlikely that the acetylated compounds may be part of the sponge cells detoxification mechanisms. These results may suggest a possible role of acetylated bile acids as a chemical defense mechanism, product of a symbiotic relationship with microorganisms, which would explain their seasonal and geographical variation, and their influence on the previously observed genotoxicity of the organic extract of S. fortis. © 2013 Elsevier Inc. All rights reserved. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v78_n10_p982_Cano http://hdl.handle.net/20.500.12110/paper_0039128X_v78_n10_p982_Cano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges bile acid cholic acid 3,7 diacetate cholic acid 3,7,12 triacetate deoxycholic acid 3,12 diacetate unclassified drug acetylation article cell viability comet assay controlled study detoxification DNA damage in vitro study metabolite nonhuman peripheral lymphocyte Siphonochalina fortis sponge (Porifera) Bacteria (microorganisms) Siphonochalina Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges Animals Cell Survival Cells, Cultured Cholic Acids Comet Assay DNA Damage Drug Evaluation, Preclinical Humans Lymphocytes Mutagens Porifera |
| spellingShingle |
Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges bile acid cholic acid 3,7 diacetate cholic acid 3,7,12 triacetate deoxycholic acid 3,12 diacetate unclassified drug acetylation article cell viability comet assay controlled study detoxification DNA damage in vitro study metabolite nonhuman peripheral lymphocyte Siphonochalina fortis sponge (Porifera) Bacteria (microorganisms) Siphonochalina Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges Animals Cell Survival Cells, Cultured Cholic Acids Comet Assay DNA Damage Drug Evaluation, Preclinical Humans Lymphocytes Mutagens Porifera Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| topic_facet |
Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges bile acid cholic acid 3,7 diacetate cholic acid 3,7,12 triacetate deoxycholic acid 3,12 diacetate unclassified drug acetylation article cell viability comet assay controlled study detoxification DNA damage in vitro study metabolite nonhuman peripheral lymphocyte Siphonochalina fortis sponge (Porifera) Bacteria (microorganisms) Siphonochalina Acetylated bile acids Bacteria Chemical defense Comet assay DNA damage Marine sponges Animals Cell Survival Cells, Cultured Cholic Acids Comet Assay DNA Damage Drug Evaluation, Preclinical Humans Lymphocytes Mutagens Porifera |
| description |
From the organic extracts of the sponge Siphonochalina fortis, collected at Bahia Bustamante, Chubut, Argentina, three major compounds were isolated and identified as deoxycholic acid 3, 12-diacetate (1), cholic acid 3, 7, 12-triacetate (2) and cholic acid, 3, 7, 12-triacetate. (3). This is the first report of acetylated bile acids in sponges and the first isolation of compound 3 as a natural product. The potential induction of DNA lesions by the isolated compounds was investigated using the comet assay in lymphocytes of human peripheral blood as in vitro model. The results showed that the administration of the bile acid derivatives would not induce DNA damages, indicating that acetylated bile acids are nontoxic metabolites at the tested concentrations. Since the free bile acids were not detected, it is unlikely that the acetylated compounds may be part of the sponge cells detoxification mechanisms. These results may suggest a possible role of acetylated bile acids as a chemical defense mechanism, product of a symbiotic relationship with microorganisms, which would explain their seasonal and geographical variation, and their influence on the previously observed genotoxicity of the organic extract of S. fortis. © 2013 Elsevier Inc. All rights reserved. |
| title |
Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| title_short |
Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| title_full |
Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| title_fullStr |
Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| title_full_unstemmed |
Isolation of acetylated bile acids from the sponge Siphonochalina fortis and DNA damage evaluation by the comet assay |
| title_sort |
isolation of acetylated bile acids from the sponge siphonochalina fortis and dna damage evaluation by the comet assay |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v78_n10_p982_Cano http://hdl.handle.net/20.500.12110/paper_0039128X_v78_n10_p982_Cano |
| _version_ |
1768543600369467392 |