A method to obtain monosporic cultures of arbuscular mycorrhizal fungi

We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermi...

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Detalles Bibliográficos
Publicado:	2001
Materias:	Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora rosea Glomus mosseae Trifolium
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v33_n9_p1283_Fracchia http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00380717_v33_n9_p1283_Fracchia
record_format	dspace
spelling	paper:paper_00380717_v33_n9_p1283_Fracchia2023-06-08T15:02:28Z A method to obtain monosporic cultures of arbuscular mycorrhizal fungi Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible. © 2001 Elsevier Science Ltd. All rights reserved. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v33_n9_p1283_Fracchia http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium
spellingShingle	Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
topic_facet	Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium
description	We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible. © 2001 Elsevier Science Ltd. All rights reserved.
title	A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_short	A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_full	A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_fullStr	A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_full_unstemmed	A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_sort	method to obtain monosporic cultures of arbuscular mycorrhizal fungi
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v33_n9_p1283_Fracchia http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia
_version_	1768541742351515648

A method to obtain monosporic cultures of arbuscular mycorrhizal fungi

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