Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats

The effect of streptozotocin diabetes on some ovarian functions in adult rats was examined. Diabetic diestrus animals showed reduced ovary weight and lower circulating levels of progesterone. Scatchard plots of binding data derived from ovarian paniculate fractions of normal and streptozotocin diabe...

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Autores principales:	Tesone, Marta, Ladenheim, Ruth Graciela, Charreau, Eduardo Hernán
Publicado:	1983
Materias:	chorionic gonadotropin i 125 chorionic gonadotropin receptor cyclic amp h 3 gonadotropin receptor insulin isophane insulin progesterone progesterone h 3 radioisotope streptozocin unclassified drug animal experiment article corpus luteum diestrus endocrine system estrus etiology female genital system intravenous drug administration nonhuman ovary insufficiency rat reproduction streptozocin diabetes Animals Binding Sites Blood Glucose Chorionic Gonadotropin Corpus Luteum Cyclic AMP Diabetes Mellitus, Experimental Female Insulin Organ Size Ovary Progesterone Rats Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v174_n1_p123_Tesone http://hdl.handle.net/20.500.12110/paper_00379727_v174_n1_p123_Tesone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00379727_v174_n1_p123_Tesone
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spelling	paper:paper_00379727_v174_n1_p123_Tesone2023-06-08T15:02:22Z Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats Tesone, Marta Ladenheim, Ruth Graciela Charreau, Eduardo Hernán chorionic gonadotropin i 125 chorionic gonadotropin receptor cyclic amp h 3 gonadotropin receptor insulin isophane insulin progesterone progesterone h 3 radioisotope streptozocin unclassified drug animal experiment article corpus luteum diestrus endocrine system estrus etiology female genital system intravenous drug administration nonhuman ovary insufficiency rat reproduction streptozocin diabetes Animals Binding Sites Blood Glucose Chorionic Gonadotropin Corpus Luteum Cyclic AMP Diabetes Mellitus, Experimental Female Insulin Organ Size Ovary Progesterone Rats Animalia The effect of streptozotocin diabetes on some ovarian functions in adult rats was examined. Diabetic diestrus animals showed reduced ovary weight and lower circulating levels of progesterone. Scatchard plots of binding data derived from ovarian paniculate fractions of normal and streptozotocin diabetic rats revealed the presence of one class of binding sites with high affinity for 125I-hCG. The apparent association constant of the hCG receptors of diabetic ovaries was comparable to that of normal gonads. However, a marked decrease (42%) in the number of hCG binding sites was found in diabetic animals. With isolated luteal cells similar results were obtained, and the administration of insulin to streptozotocin diabetic rats restored to normality the number of hCG binding sites. The maximal response of progesterone production by luteal cells from control ovaries was obtained with 10-10 M hCG. A 100-fold higher concentration of hCG was required for the maximum stimulation of cAMP synthesis. The cAMP response of cells from diabetic rats was significantly higher than that of control cells. However, luteal cells from diabetic rats showed some loss of sensitivity in the synthesis of progesterone during incubation with hCG. Most of the alterations seen in diabetic female rats could be restored with insulin therapy, indicating that insulin plays an important role in the regulation and maintenance of normal reproductive functions. It is suggested that the diminution of the LH receptor population causes the disruption of normal luteal cell function. This fact could be responsible for some of the reproductive alterations in the diabetic female rat. © 1983, SAGE Publications. All rights reserved. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ladenheim, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v174_n1_p123_Tesone http://hdl.handle.net/20.500.12110/paper_00379727_v174_n1_p123_Tesone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	chorionic gonadotropin i 125 chorionic gonadotropin receptor cyclic amp h 3 gonadotropin receptor insulin isophane insulin progesterone progesterone h 3 radioisotope streptozocin unclassified drug animal experiment article corpus luteum diestrus endocrine system estrus etiology female genital system intravenous drug administration nonhuman ovary insufficiency rat reproduction streptozocin diabetes Animals Binding Sites Blood Glucose Chorionic Gonadotropin Corpus Luteum Cyclic AMP Diabetes Mellitus, Experimental Female Insulin Organ Size Ovary Progesterone Rats Animalia
spellingShingle	chorionic gonadotropin i 125 chorionic gonadotropin receptor cyclic amp h 3 gonadotropin receptor insulin isophane insulin progesterone progesterone h 3 radioisotope streptozocin unclassified drug animal experiment article corpus luteum diestrus endocrine system estrus etiology female genital system intravenous drug administration nonhuman ovary insufficiency rat reproduction streptozocin diabetes Animals Binding Sites Blood Glucose Chorionic Gonadotropin Corpus Luteum Cyclic AMP Diabetes Mellitus, Experimental Female Insulin Organ Size Ovary Progesterone Rats Animalia Tesone, Marta Ladenheim, Ruth Graciela Charreau, Eduardo Hernán Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
topic_facet	chorionic gonadotropin i 125 chorionic gonadotropin receptor cyclic amp h 3 gonadotropin receptor insulin isophane insulin progesterone progesterone h 3 radioisotope streptozocin unclassified drug animal experiment article corpus luteum diestrus endocrine system estrus etiology female genital system intravenous drug administration nonhuman ovary insufficiency rat reproduction streptozocin diabetes Animals Binding Sites Blood Glucose Chorionic Gonadotropin Corpus Luteum Cyclic AMP Diabetes Mellitus, Experimental Female Insulin Organ Size Ovary Progesterone Rats Animalia
description	The effect of streptozotocin diabetes on some ovarian functions in adult rats was examined. Diabetic diestrus animals showed reduced ovary weight and lower circulating levels of progesterone. Scatchard plots of binding data derived from ovarian paniculate fractions of normal and streptozotocin diabetic rats revealed the presence of one class of binding sites with high affinity for 125I-hCG. The apparent association constant of the hCG receptors of diabetic ovaries was comparable to that of normal gonads. However, a marked decrease (42%) in the number of hCG binding sites was found in diabetic animals. With isolated luteal cells similar results were obtained, and the administration of insulin to streptozotocin diabetic rats restored to normality the number of hCG binding sites. The maximal response of progesterone production by luteal cells from control ovaries was obtained with 10-10 M hCG. A 100-fold higher concentration of hCG was required for the maximum stimulation of cAMP synthesis. The cAMP response of cells from diabetic rats was significantly higher than that of control cells. However, luteal cells from diabetic rats showed some loss of sensitivity in the synthesis of progesterone during incubation with hCG. Most of the alterations seen in diabetic female rats could be restored with insulin therapy, indicating that insulin plays an important role in the regulation and maintenance of normal reproductive functions. It is suggested that the diminution of the LH receptor population causes the disruption of normal luteal cell function. This fact could be responsible for some of the reproductive alterations in the diabetic female rat. © 1983, SAGE Publications. All rights reserved.
author	Tesone, Marta Ladenheim, Ruth Graciela Charreau, Eduardo Hernán
author_facet	Tesone, Marta Ladenheim, Ruth Graciela Charreau, Eduardo Hernán
author_sort	Tesone, Marta
title	Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
title_short	Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
title_full	Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
title_fullStr	Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
title_full_unstemmed	Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats
title_sort	ovarian dysfunction in streptozotocin-lnduced diabetic rats
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v174_n1_p123_Tesone http://hdl.handle.net/20.500.12110/paper_00379727_v174_n1_p123_Tesone
work_keys_str_mv	AT tesonemarta ovariandysfunctioninstreptozotocinlnduceddiabeticrats AT ladenheimruthgraciela ovariandysfunctioninstreptozotocinlnduceddiabeticrats AT charreaueduardohernan ovariandysfunctioninstreptozotocinlnduceddiabeticrats
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Ovarian Dysfunction in Streptozotocin-lnduced Diabetic Rats

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