Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices

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Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate f...

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Autores principales:	Martinez, Alicia Elba, Chiocchio, Viviana Mónica, Rodríguez, María Alejandra, Godeas, Alicia Margarita
Publicado:	2012
Materias:	Arum colonization Glomus intraradices Paris colonization Pteridophyta-gametophyte-sporophyte Pteris vittata article bacterial spore brake fern flower Glomeromycota microbiology mycorrhiza physiology Germ Cells, Plant Mycorrhizae Pteris Spores Adiantum Arbuscular Arum Bacteria (microorganisms) Filicophyta Fungi Pteridaceae Pteridium Pteridophyta
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347744_v60_n2_p857_Martinez http://hdl.handle.net/20.500.12110/paper_00347744_v60_n2_p857_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00347744_v60_n2_p857_Martinez
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spelling	paper:paper_00347744_v60_n2_p857_Martinez2023-06-08T15:01:04Z Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices Martinez, Alicia Elba Chiocchio, Viviana Mónica Rodríguez, María Alejandra Godeas, Alicia Margarita Arum colonization Glomus intraradices Paris colonization Pteridophyta-gametophyte-sporophyte Pteris vittata article bacterial spore brake fern flower Glomeromycota microbiology mycorrhiza physiology Germ Cells, Plant Glomeromycota Mycorrhizae Pteris Spores Adiantum Arbuscular Arum Bacteria (microorganisms) Filicophyta Fungi Glomus intraradices Pteridaceae Pteridium Pteridophyta Pteris Pteris vittata Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC N° 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions. Fil:Martinez, A.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chiocchio, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347744_v60_n2_p857_Martinez http://hdl.handle.net/20.500.12110/paper_00347744_v60_n2_p857_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arum colonization Glomus intraradices Paris colonization Pteridophyta-gametophyte-sporophyte Pteris vittata article bacterial spore brake fern flower Glomeromycota microbiology mycorrhiza physiology Germ Cells, Plant Glomeromycota Mycorrhizae Pteris Spores Adiantum Arbuscular Arum Bacteria (microorganisms) Filicophyta Fungi Glomus intraradices Pteridaceae Pteridium Pteridophyta Pteris Pteris vittata
spellingShingle	Arum colonization Glomus intraradices Paris colonization Pteridophyta-gametophyte-sporophyte Pteris vittata article bacterial spore brake fern flower Glomeromycota microbiology mycorrhiza physiology Germ Cells, Plant Glomeromycota Mycorrhizae Pteris Spores Adiantum Arbuscular Arum Bacteria (microorganisms) Filicophyta Fungi Glomus intraradices Pteridaceae Pteridium Pteridophyta Pteris Pteris vittata Martinez, Alicia Elba Chiocchio, Viviana Mónica Rodríguez, María Alejandra Godeas, Alicia Margarita Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
topic_facet	Arum colonization Glomus intraradices Paris colonization Pteridophyta-gametophyte-sporophyte Pteris vittata article bacterial spore brake fern flower Glomeromycota microbiology mycorrhiza physiology Germ Cells, Plant Glomeromycota Mycorrhizae Pteris Spores Adiantum Arbuscular Arum Bacteria (microorganisms) Filicophyta Fungi Glomus intraradices Pteridaceae Pteridium Pteridophyta Pteris Pteris vittata
description	Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC N° 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions.
author	Martinez, Alicia Elba Chiocchio, Viviana Mónica Rodríguez, María Alejandra Godeas, Alicia Margarita
author_facet	Martinez, Alicia Elba Chiocchio, Viviana Mónica Rodríguez, María Alejandra Godeas, Alicia Margarita
author_sort	Martinez, Alicia Elba
title	Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
title_short	Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
title_full	Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
title_fullStr	Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
title_full_unstemmed	Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
title_sort	mycorrhizal association in gametophytes and sporophytes of the fern pteris vittata (pteridaceae) with glomus intraradices
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347744_v60_n2_p857_Martinez http://hdl.handle.net/20.500.12110/paper_00347744_v60_n2_p857_Martinez
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_version_	1768542542656176128

Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices

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