The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutam...
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2012
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paper:paper_00320935_v236_n6_p1831_Grandellis2023-06-08T15:00:05Z The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs Grandellis, Carolina Giammaria, Verónica Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutamines, which presents myristoylation and palmitoylation consensus sites and a PEST sequence indicative of rapid protein degradation. StCDPK3 gene (circa 11 kb) is localized in chromosome 3, shares the eight exons and seven introns structure with other isoforms from subgroup IIa and contains an additional intron in the 5′UTR region. StCDPK3 expression is ubiquitous being transcripts more abundant in early elongating stolons (ES), leaves and roots, however isoform specific antibodies only detected the protein in leaf particulate extracts. The recombinant 6xHis-StCDPK3 is an active kinase that differs in its kinetic parameters and calcium requirements from StCDPK1 and 2 isoforms. In vitro, StCDPK3 undergoes autophosphorylation regardless of the addition of calcium. The StCDPK3 promoter region (circa 1,800 bp) was subcloned by genome walking and fused to GUS. Light and ABRE responsive elements were identified in the promoter region as well as elements associated to expression in roots. StCDPK3 expression was enhanced by ABA while GA decreased it. Potato transgenic lines harboring StCDPK3 promoter{proportion}GUS construct were generated by Agrobacterium tumefaciens mediated plant transformation. Promoter activity was detected in leaves, root tips and branching points, early ES, tuber eyes and developing sprouts indicating that StCDPK3 is expressed in actively growing organs. © 2012 Springer-Verlag. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v236_n6_p1831_Grandellis http://hdl.handle.net/20.500.12110/paper_00320935_v236_n6_p1831_Grandellis |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum |
| spellingShingle |
Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum Grandellis, Carolina Giammaria, Verónica The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| topic_facet |
Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum |
| description |
Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutamines, which presents myristoylation and palmitoylation consensus sites and a PEST sequence indicative of rapid protein degradation. StCDPK3 gene (circa 11 kb) is localized in chromosome 3, shares the eight exons and seven introns structure with other isoforms from subgroup IIa and contains an additional intron in the 5′UTR region. StCDPK3 expression is ubiquitous being transcripts more abundant in early elongating stolons (ES), leaves and roots, however isoform specific antibodies only detected the protein in leaf particulate extracts. The recombinant 6xHis-StCDPK3 is an active kinase that differs in its kinetic parameters and calcium requirements from StCDPK1 and 2 isoforms. In vitro, StCDPK3 undergoes autophosphorylation regardless of the addition of calcium. The StCDPK3 promoter region (circa 1,800 bp) was subcloned by genome walking and fused to GUS. Light and ABRE responsive elements were identified in the promoter region as well as elements associated to expression in roots. StCDPK3 expression was enhanced by ABA while GA decreased it. Potato transgenic lines harboring StCDPK3 promoter{proportion}GUS construct were generated by Agrobacterium tumefaciens mediated plant transformation. Promoter activity was detected in leaves, root tips and branching points, early ES, tuber eyes and developing sprouts indicating that StCDPK3 is expressed in actively growing organs. © 2012 Springer-Verlag. |
| author |
Grandellis, Carolina Giammaria, Verónica |
| author_facet |
Grandellis, Carolina Giammaria, Verónica |
| author_sort |
Grandellis, Carolina |
| title |
The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| title_short |
The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| title_full |
The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| title_fullStr |
The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| title_full_unstemmed |
The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs |
| title_sort |
novel solanum tuberosum calcium dependent protein kinase, stcdpk3, is expressed in actively growing organs |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v236_n6_p1831_Grandellis http://hdl.handle.net/20.500.12110/paper_00320935_v236_n6_p1831_Grandellis |
| work_keys_str_mv |
AT grandelliscarolina thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT giammariaveronica thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT grandelliscarolina novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT giammariaveronica novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans |
| _version_ |
1768543409269637120 |