Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro

Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene (besides the selective gene aadA) has been published. We report here successful expression of β-glucuronidase in transplastomic Solanum tuberosum (var....

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Autores principales:	Segretin, María Eugenia, Lentz, Ezequiel Matías, Wirth, Sonia Alejandra, Morgenfeld, Mauro Miguel
Publicado:	2012
Materias:	β-Glucuronidase Chloroplast Plastid transformation Potato Tobacco Tuber beta glucuronidase recombinant protein vegetable protein article biosynthesis comparative study enzymology gene expression regulation genetics plant leaf plant tuber plastid potato tobacco transgene transgenic plant Gene Expression Regulation, Plant Glucuronidase Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Plastids Recombinant Proteins Solanum tuberosum Transgenes Nicotiana tabacum Tuber (truffle)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v235_n4_p807_Segretin http://hdl.handle.net/20.500.12110/paper_00320935_v235_n4_p807_Segretin
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00320935_v235_n4_p807_Segretin
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spelling	paper:paper_00320935_v235_n4_p807_Segretin2023-06-08T15:00:04Z Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro Segretin, María Eugenia Lentz, Ezequiel Matías Wirth, Sonia Alejandra Morgenfeld, Mauro Miguel β-Glucuronidase Chloroplast Plastid transformation Potato Tobacco Tuber beta glucuronidase recombinant protein vegetable protein article biosynthesis comparative study enzymology gene expression regulation genetics plant leaf plant tuber plastid potato tobacco transgene transgenic plant Gene Expression Regulation, Plant Glucuronidase Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Plastids Recombinant Proteins Solanum tuberosum Tobacco Transgenes Nicotiana tabacum Solanum tuberosum Tuber (truffle) Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene (besides the selective gene aadA) has been published. We report here successful expression of β-glucuronidase in transplastomic Solanum tuberosum (var. Desiree) plants, with accumulation levels for the recombinant protein of up to 41% of total soluble protein in mature leaves. To our knowledge, this is the highest expression level reported for a heterologous protein in S. tuberosum. Accumulation of the recombinant protein in soil-grown minitubers was very low, as described in previous reports. Interestingly, microtubers developed in vitro showed higher accumulation of β-glucuronidase. As light exposure during their development could be the trigger for this high accumulation, we analyzed the effect of light on β-glucuronidase accumulation in transplastomic tubers. Exposure to light for 8 days increased β-glucuronidase accumulation in soil-grown tubers, acting as a light-inducible expression system for recombinant protein accumulation in tuber plastids. In this paper we show that plastid transformation in potato allows the highest recombinant protein accumulation in foliar tissue described so far for this food crop. We also demonstrate that in tubers high accumulation is possible and depends on light exposure. Because tubers have many advantages as protein storage organs, these results could lead to new recombinant protein production schemes based on potato. © 2011 Springer-Verlag. Fil:Segretin, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lentz, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wirth, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Morgenfeld, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v235_n4_p807_Segretin http://hdl.handle.net/20.500.12110/paper_00320935_v235_n4_p807_Segretin
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-Glucuronidase Chloroplast Plastid transformation Potato Tobacco Tuber beta glucuronidase recombinant protein vegetable protein article biosynthesis comparative study enzymology gene expression regulation genetics plant leaf plant tuber plastid potato tobacco transgene transgenic plant Gene Expression Regulation, Plant Glucuronidase Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Plastids Recombinant Proteins Solanum tuberosum Tobacco Transgenes Nicotiana tabacum Solanum tuberosum Tuber (truffle)
spellingShingle	β-Glucuronidase Chloroplast Plastid transformation Potato Tobacco Tuber beta glucuronidase recombinant protein vegetable protein article biosynthesis comparative study enzymology gene expression regulation genetics plant leaf plant tuber plastid potato tobacco transgene transgenic plant Gene Expression Regulation, Plant Glucuronidase Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Plastids Recombinant Proteins Solanum tuberosum Tobacco Transgenes Nicotiana tabacum Solanum tuberosum Tuber (truffle) Segretin, María Eugenia Lentz, Ezequiel Matías Wirth, Sonia Alejandra Morgenfeld, Mauro Miguel Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
topic_facet	β-Glucuronidase Chloroplast Plastid transformation Potato Tobacco Tuber beta glucuronidase recombinant protein vegetable protein article biosynthesis comparative study enzymology gene expression regulation genetics plant leaf plant tuber plastid potato tobacco transgene transgenic plant Gene Expression Regulation, Plant Glucuronidase Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Plastids Recombinant Proteins Solanum tuberosum Tobacco Transgenes Nicotiana tabacum Solanum tuberosum Tuber (truffle)
description	Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene (besides the selective gene aadA) has been published. We report here successful expression of β-glucuronidase in transplastomic Solanum tuberosum (var. Desiree) plants, with accumulation levels for the recombinant protein of up to 41% of total soluble protein in mature leaves. To our knowledge, this is the highest expression level reported for a heterologous protein in S. tuberosum. Accumulation of the recombinant protein in soil-grown minitubers was very low, as described in previous reports. Interestingly, microtubers developed in vitro showed higher accumulation of β-glucuronidase. As light exposure during their development could be the trigger for this high accumulation, we analyzed the effect of light on β-glucuronidase accumulation in transplastomic tubers. Exposure to light for 8 days increased β-glucuronidase accumulation in soil-grown tubers, acting as a light-inducible expression system for recombinant protein accumulation in tuber plastids. In this paper we show that plastid transformation in potato allows the highest recombinant protein accumulation in foliar tissue described so far for this food crop. We also demonstrate that in tubers high accumulation is possible and depends on light exposure. Because tubers have many advantages as protein storage organs, these results could lead to new recombinant protein production schemes based on potato. © 2011 Springer-Verlag.
author	Segretin, María Eugenia Lentz, Ezequiel Matías Wirth, Sonia Alejandra Morgenfeld, Mauro Miguel
author_facet	Segretin, María Eugenia Lentz, Ezequiel Matías Wirth, Sonia Alejandra Morgenfeld, Mauro Miguel
author_sort	Segretin, María Eugenia
title	Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
title_short	Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
title_full	Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
title_fullStr	Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
title_full_unstemmed	Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
title_sort	transformation of solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v235_n4_p807_Segretin http://hdl.handle.net/20.500.12110/paper_00320935_v235_n4_p807_Segretin
work_keys_str_mv	AT segretinmariaeugenia transformationofsolanumtuberosumplastidsallowshighexpressionlevelsofbglucuronidasebothinleavesandmicrotubersdevelopedinvitro AT lentzezequielmatias transformationofsolanumtuberosumplastidsallowshighexpressionlevelsofbglucuronidasebothinleavesandmicrotubersdevelopedinvitro AT wirthsoniaalejandra transformationofsolanumtuberosumplastidsallowshighexpressionlevelsofbglucuronidasebothinleavesandmicrotubersdevelopedinvitro AT morgenfeldmauromiguel transformationofsolanumtuberosumplastidsallowshighexpressionlevelsofbglucuronidasebothinleavesandmicrotubersdevelopedinvitro
_version_	1768543741992239104

Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro

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