A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants
A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v128_n1_p256_Chico http://hdl.handle.net/20.500.12110/paper_00320889_v128_n1_p256_Chico |
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paper:paper_00320889_v128_n1_p256_Chico2023-06-08T14:59:58Z A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants Amino acids Biological membranes Calcium DNA Enzyme kinetics Physiology Plant cell culture Proteins RNA Defense mechanisms Plants (botany) Cladosporium fulvum Cladosporium fulvum Embryophyta Lycopersicon esculentum Lycopersicon esculentum calcium binding protein messenger RNA protein kinase adaptation amino acid sequence article Colletotrichum enzymology gene expression regulation genetics growth, development and aging innate immunity isolation and purification metabolism microbiology molecular cloning molecular genetics Northern blotting plant disease plant leaf sequence homology signal transduction Southern blotting tomato Western blotting Adaptation, Physiological Amino Acid Sequence Blotting, Northern Blotting, Southern Blotting, Western Calcium-Binding Proteins Cloning, Molecular Colletotrichum Gene Expression Regulation, Enzymologic Immunity, Natural Lycopersicon esculentum Molecular Sequence Data Plant Diseases Plant Leaves Protein Kinases RNA, Messenger Sequence Homology, Amino Acid Signal Transduction A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v128_n1_p256_Chico http://hdl.handle.net/20.500.12110/paper_00320889_v128_n1_p256_Chico |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amino acids Biological membranes Calcium DNA Enzyme kinetics Physiology Plant cell culture Proteins RNA Defense mechanisms Plants (botany) Cladosporium fulvum Cladosporium fulvum Embryophyta Lycopersicon esculentum Lycopersicon esculentum calcium binding protein messenger RNA protein kinase adaptation amino acid sequence article Colletotrichum enzymology gene expression regulation genetics growth, development and aging innate immunity isolation and purification metabolism microbiology molecular cloning molecular genetics Northern blotting plant disease plant leaf sequence homology signal transduction Southern blotting tomato Western blotting Adaptation, Physiological Amino Acid Sequence Blotting, Northern Blotting, Southern Blotting, Western Calcium-Binding Proteins Cloning, Molecular Colletotrichum Gene Expression Regulation, Enzymologic Immunity, Natural Lycopersicon esculentum Molecular Sequence Data Plant Diseases Plant Leaves Protein Kinases RNA, Messenger Sequence Homology, Amino Acid Signal Transduction |
| spellingShingle |
Amino acids Biological membranes Calcium DNA Enzyme kinetics Physiology Plant cell culture Proteins RNA Defense mechanisms Plants (botany) Cladosporium fulvum Cladosporium fulvum Embryophyta Lycopersicon esculentum Lycopersicon esculentum calcium binding protein messenger RNA protein kinase adaptation amino acid sequence article Colletotrichum enzymology gene expression regulation genetics growth, development and aging innate immunity isolation and purification metabolism microbiology molecular cloning molecular genetics Northern blotting plant disease plant leaf sequence homology signal transduction Southern blotting tomato Western blotting Adaptation, Physiological Amino Acid Sequence Blotting, Northern Blotting, Southern Blotting, Western Calcium-Binding Proteins Cloning, Molecular Colletotrichum Gene Expression Regulation, Enzymologic Immunity, Natural Lycopersicon esculentum Molecular Sequence Data Plant Diseases Plant Leaves Protein Kinases RNA, Messenger Sequence Homology, Amino Acid Signal Transduction A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| topic_facet |
Amino acids Biological membranes Calcium DNA Enzyme kinetics Physiology Plant cell culture Proteins RNA Defense mechanisms Plants (botany) Cladosporium fulvum Cladosporium fulvum Embryophyta Lycopersicon esculentum Lycopersicon esculentum calcium binding protein messenger RNA protein kinase adaptation amino acid sequence article Colletotrichum enzymology gene expression regulation genetics growth, development and aging innate immunity isolation and purification metabolism microbiology molecular cloning molecular genetics Northern blotting plant disease plant leaf sequence homology signal transduction Southern blotting tomato Western blotting Adaptation, Physiological Amino Acid Sequence Blotting, Northern Blotting, Southern Blotting, Western Calcium-Binding Proteins Cloning, Molecular Colletotrichum Gene Expression Regulation, Enzymologic Immunity, Natural Lycopersicon esculentum Molecular Sequence Data Plant Diseases Plant Leaves Protein Kinases RNA, Messenger Sequence Homology, Amino Acid Signal Transduction |
| description |
A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks. |
| title |
A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| title_short |
A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| title_full |
A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| title_fullStr |
A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| title_full_unstemmed |
A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| title_sort |
calcium-dependent protein kinase is systemically induced upon wounding in tomato plants |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v128_n1_p256_Chico http://hdl.handle.net/20.500.12110/paper_00320889_v128_n1_p256_Chico |
| _version_ |
1768545454158512128 |