Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells

The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissu...

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Detalles Bibliográficos
Autores principales: Catz, Diana Silvia, Moreno, Silvia, Tandecarz, Juana Sara
Publicado: 1989
Materias:
Callus
dedifferentiation
isoenzymes
phosphorylase
potato
Solanum tuberosum
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v76_n2_p221_Catz
http://hdl.handle.net/20.500.12110/paper_00319317_v76_n2_p221_Catz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00319317_v76_n2_p221_Catz
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spelling paper:paper_00319317_v76_n2_p221_Catz2023-06-08T14:59:00Z Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells Catz, Diana Silvia Moreno, Silvia Tandecarz, Juana Sara Callus dedifferentiation isoenzymes phosphorylase potato Solanum tuberosum The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissue contains a single form of phosphorylase that differs in primer requirement, charge and affinity towards branched α‐1,4/1,6‐glucans from the major phosphorylase form (phosphorylase II) in potato tuber. This latter molecular form is missing in dedifferentiated callus. However, callus phosphorylase appears to be closely related to tuber phosphorylase I, a minor form found in the original explant tissue. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:Catz, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v76_n2_p221_Catz http://hdl.handle.net/20.500.12110/paper_00319317_v76_n2_p221_Catz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Callus
dedifferentiation
isoenzymes
phosphorylase
potato
Solanum tuberosum
spellingShingle Callus
dedifferentiation
isoenzymes
phosphorylase
potato
Solanum tuberosum
Catz, Diana Silvia
Moreno, Silvia
Tandecarz, Juana Sara
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
topic_facet Callus
dedifferentiation
isoenzymes
phosphorylase
potato
Solanum tuberosum
description The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissue contains a single form of phosphorylase that differs in primer requirement, charge and affinity towards branched α‐1,4/1,6‐glucans from the major phosphorylase form (phosphorylase II) in potato tuber. This latter molecular form is missing in dedifferentiated callus. However, callus phosphorylase appears to be closely related to tuber phosphorylase I, a minor form found in the original explant tissue. Copyright © 1989, Wiley Blackwell. All rights reserved
author Catz, Diana Silvia
Moreno, Silvia
Tandecarz, Juana Sara
author_facet Catz, Diana Silvia
Moreno, Silvia
Tandecarz, Juana Sara
author_sort Catz, Diana Silvia
title Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
title_short Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
title_full Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
title_fullStr Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
title_full_unstemmed Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
title_sort change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
publishDate 1989
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v76_n2_p221_Catz
http://hdl.handle.net/20.500.12110/paper_00319317_v76_n2_p221_Catz
work_keys_str_mv AT catzdianasilvia changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells
AT morenosilvia changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells
AT tandecarzjuanasara changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells
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