Superresolution imaging with switchable fluorophores based on oxazine auxochromes

The spatial resolution of fluorescence microscopes is limited by diffraction to about half of the light wavelength, hampering the observation of many important intracellular processes. Recent emerging techniques have overcome that diffraction barrier using the temporal discrimination of close object...

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Detalles Bibliográficos
Autores principales:	Roberti, Maria Julia, Bossi, Mariano Luis
Publicado:	2013
Materias:	fluorescent dye oxazine derivative Fluorescent Dyes Oxazines
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1391_Petriella http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1391_Petriella
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00318655_v89_n6_p1391_Petriella
record_format	dspace
spelling	paper:paper_00318655_v89_n6_p1391_Petriella2023-06-08T14:57:31Z Superresolution imaging with switchable fluorophores based on oxazine auxochromes Roberti, Maria Julia Bossi, Mariano Luis fluorescent dye oxazine derivative Fluorescent Dyes Oxazines The spatial resolution of fluorescence microscopes is limited by diffraction to about half of the light wavelength, hampering the observation of many important intracellular processes. Recent emerging techniques have overcome that diffraction barrier using the temporal discrimination of close objects that are otherwise unresolved or blurred within the spatial resolution of the microscope. The key of these techniques is to switch the signal of fluorescence markers on and off exploiting their distinct molecular states, and detect and localize these markers at the single-molecule level. This underlying principle highlights the critical role of the photophysical properties of the probes, and the importance of finding adequate switching mechanisms. Here, we present strategies to achieve fluorescence modulation based on novel molecular assemblies containing a [1,3]oxazine as the two states, building block responsible for the transformation. Two different triggering events, based on the photochromic and halochromic properties of the oxazine, induce a large absorption and emission bathochromic shift of a pendant fluorophore, as the ultimate fluorescence switching event. The implementation of these approaches to achieve spatial resolution beyond the diffraction limit is also discussed. © 2013 The American Society of Photobiology. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bossi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1391_Petriella http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1391_Petriella
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	fluorescent dye oxazine derivative Fluorescent Dyes Oxazines
spellingShingle	fluorescent dye oxazine derivative Fluorescent Dyes Oxazines Roberti, Maria Julia Bossi, Mariano Luis Superresolution imaging with switchable fluorophores based on oxazine auxochromes
topic_facet	fluorescent dye oxazine derivative Fluorescent Dyes Oxazines
description	The spatial resolution of fluorescence microscopes is limited by diffraction to about half of the light wavelength, hampering the observation of many important intracellular processes. Recent emerging techniques have overcome that diffraction barrier using the temporal discrimination of close objects that are otherwise unresolved or blurred within the spatial resolution of the microscope. The key of these techniques is to switch the signal of fluorescence markers on and off exploiting their distinct molecular states, and detect and localize these markers at the single-molecule level. This underlying principle highlights the critical role of the photophysical properties of the probes, and the importance of finding adequate switching mechanisms. Here, we present strategies to achieve fluorescence modulation based on novel molecular assemblies containing a [1,3]oxazine as the two states, building block responsible for the transformation. Two different triggering events, based on the photochromic and halochromic properties of the oxazine, induce a large absorption and emission bathochromic shift of a pendant fluorophore, as the ultimate fluorescence switching event. The implementation of these approaches to achieve spatial resolution beyond the diffraction limit is also discussed. © 2013 The American Society of Photobiology.
author	Roberti, Maria Julia Bossi, Mariano Luis
author_facet	Roberti, Maria Julia Bossi, Mariano Luis
author_sort	Roberti, Maria Julia
title	Superresolution imaging with switchable fluorophores based on oxazine auxochromes
title_short	Superresolution imaging with switchable fluorophores based on oxazine auxochromes
title_full	Superresolution imaging with switchable fluorophores based on oxazine auxochromes
title_fullStr	Superresolution imaging with switchable fluorophores based on oxazine auxochromes
title_full_unstemmed	Superresolution imaging with switchable fluorophores based on oxazine auxochromes
title_sort	superresolution imaging with switchable fluorophores based on oxazine auxochromes
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1391_Petriella http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1391_Petriella
work_keys_str_mv	AT robertimariajulia superresolutionimagingwithswitchablefluorophoresbasedonoxazineauxochromes AT bossimarianoluis superresolutionimagingwithswitchablefluorophoresbasedonoxazineauxochromes
_version_	1768543645508567040

Superresolution imaging with switchable fluorophores based on oxazine auxochromes

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