Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluoresce...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1383_Novo http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1383_Novo |
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paper:paper_00318655_v89_n6_p1383_Novo2023-06-08T14:57:30Z Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment Iriel, Analía Marchi, María Claudia Lagorio, María Gabriela Origanum Origanum vulgare chemistry fluorescence microscopy Origanum procedures scanning electron microscopy spectrofluorometry Microscopy, Electron, Scanning Microscopy, Fluorescence Origanum Spectrometry, Fluorescence The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology. Fil:Iriel, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Claudia Marchi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabriela Lagorio, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1383_Novo http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1383_Novo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Origanum Origanum vulgare chemistry fluorescence microscopy Origanum procedures scanning electron microscopy spectrofluorometry Microscopy, Electron, Scanning Microscopy, Fluorescence Origanum Spectrometry, Fluorescence |
| spellingShingle |
Origanum Origanum vulgare chemistry fluorescence microscopy Origanum procedures scanning electron microscopy spectrofluorometry Microscopy, Electron, Scanning Microscopy, Fluorescence Origanum Spectrometry, Fluorescence Iriel, Analía Marchi, María Claudia Lagorio, María Gabriela Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| topic_facet |
Origanum Origanum vulgare chemistry fluorescence microscopy Origanum procedures scanning electron microscopy spectrofluorometry Microscopy, Electron, Scanning Microscopy, Fluorescence Origanum Spectrometry, Fluorescence |
| description |
The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology. |
| author |
Iriel, Analía Marchi, María Claudia Lagorio, María Gabriela |
| author_facet |
Iriel, Analía Marchi, María Claudia Lagorio, María Gabriela |
| author_sort |
Iriel, Analía |
| title |
Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| title_short |
Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| title_full |
Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| title_fullStr |
Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| title_full_unstemmed |
Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment |
| title_sort |
spectroscopy, microscopy and fluorescence imaging of origanum vulgare l. basis for nondestructive quality assessment |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1383_Novo http://hdl.handle.net/20.500.12110/paper_00318655_v89_n6_p1383_Novo |
| work_keys_str_mv |
AT irielanalia spectroscopymicroscopyandfluorescenceimagingoforiganumvulgarelbasisfornondestructivequalityassessment AT marchimariaclaudia spectroscopymicroscopyandfluorescenceimagingoforiganumvulgarelbasisfornondestructivequalityassessment AT lagoriomariagabriela spectroscopymicroscopyandfluorescenceimagingoforiganumvulgarelbasisfornondestructivequalityassessment |
| _version_ |
1768543170704965632 |