Visualization of fast calcium oscillations in the parafascicular nucleus
The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the "non-specific" intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident...
| Publicado: |
2013
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00316768_v465_n9_p1327_Hyde http://hdl.handle.net/20.500.12110/paper_00316768_v465_n9_p1327_Hyde |
| Aporte de: |
| id |
paper:paper_00316768_v465_n9_p1327_Hyde |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00316768_v465_n9_p1327_Hyde2023-06-08T14:57:04Z Visualization of fast calcium oscillations in the parafascicular nucleus Arousal Gamma band activity Intralaminar thalamus P/Q-type channels cadmium calcium ion ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid omega agatoxin IVA omega conotoxin GVIA animal cell article brain function calcium signaling calcium transport controlled study dendrite depolarization electroencephalogram fluorescence image analysis molecular imaging nerve cell nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Action Potentials Animals Calcium Channel Blockers Calcium Signaling Intralaminar Thalamic Nuclei Microscopy, Fluorescence, Multiphoton Neurons Rats Rats, Sprague-Dawley The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the "non-specific" intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems. © 2013 Springer-Verlag Berlin Heidelberg. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00316768_v465_n9_p1327_Hyde http://hdl.handle.net/20.500.12110/paper_00316768_v465_n9_p1327_Hyde |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Gamma band activity Intralaminar thalamus P/Q-type channels cadmium calcium ion ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid omega agatoxin IVA omega conotoxin GVIA animal cell article brain function calcium signaling calcium transport controlled study dendrite depolarization electroencephalogram fluorescence image analysis molecular imaging nerve cell nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Action Potentials Animals Calcium Channel Blockers Calcium Signaling Intralaminar Thalamic Nuclei Microscopy, Fluorescence, Multiphoton Neurons Rats Rats, Sprague-Dawley |
| spellingShingle |
Arousal Gamma band activity Intralaminar thalamus P/Q-type channels cadmium calcium ion ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid omega agatoxin IVA omega conotoxin GVIA animal cell article brain function calcium signaling calcium transport controlled study dendrite depolarization electroencephalogram fluorescence image analysis molecular imaging nerve cell nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Action Potentials Animals Calcium Channel Blockers Calcium Signaling Intralaminar Thalamic Nuclei Microscopy, Fluorescence, Multiphoton Neurons Rats Rats, Sprague-Dawley Visualization of fast calcium oscillations in the parafascicular nucleus |
| topic_facet |
Arousal Gamma band activity Intralaminar thalamus P/Q-type channels cadmium calcium ion ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid omega agatoxin IVA omega conotoxin GVIA animal cell article brain function calcium signaling calcium transport controlled study dendrite depolarization electroencephalogram fluorescence image analysis molecular imaging nerve cell nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Action Potentials Animals Calcium Channel Blockers Calcium Signaling Intralaminar Thalamic Nuclei Microscopy, Fluorescence, Multiphoton Neurons Rats Rats, Sprague-Dawley |
| description |
The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the "non-specific" intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems. © 2013 Springer-Verlag Berlin Heidelberg. |
| title |
Visualization of fast calcium oscillations in the parafascicular nucleus |
| title_short |
Visualization of fast calcium oscillations in the parafascicular nucleus |
| title_full |
Visualization of fast calcium oscillations in the parafascicular nucleus |
| title_fullStr |
Visualization of fast calcium oscillations in the parafascicular nucleus |
| title_full_unstemmed |
Visualization of fast calcium oscillations in the parafascicular nucleus |
| title_sort |
visualization of fast calcium oscillations in the parafascicular nucleus |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00316768_v465_n9_p1327_Hyde http://hdl.handle.net/20.500.12110/paper_00316768_v465_n9_p1327_Hyde |
| _version_ |
1768546711906549760 |