Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina
Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n4_p516_Cura http://hdl.handle.net/20.500.12110/paper_00311820_v139_n4_p516_Cura |
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paper:paper_00311820_v139_n4_p516_Cura2023-06-08T14:56:58Z Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina Chagas disease Discrete Typing Unit PCR Trypanosoma cruzi adolescent adult aged Argentina article bloodstream infection Chagas disease child chronicity endemic disease geographic distribution graft recipient human human tissue immunocompromised patient major clinical study pathogenesis polymerase chain reaction priority journal skin biopsy taxonomy tissue distribution Trypanosoma cruzi Adolescent Adult Aged Aged, 80 and over Argentina Chagas Cardiomyopathy Chagas Disease Child Child, Preschool DNA, Protozoan Endemic Diseases Female Genetic Variation Genotype Heart Humans Infant Male Middle Aged Phylogeny Polymerase Chain Reaction Trypanosoma cruzi Young Adult Trypanosoma cruzi Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population. © Copyright 2012 Published by Cambridge University Press. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n4_p516_Cura http://hdl.handle.net/20.500.12110/paper_00311820_v139_n4_p516_Cura |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Discrete Typing Unit PCR Trypanosoma cruzi adolescent adult aged Argentina article bloodstream infection Chagas disease child chronicity endemic disease geographic distribution graft recipient human human tissue immunocompromised patient major clinical study pathogenesis polymerase chain reaction priority journal skin biopsy taxonomy tissue distribution Trypanosoma cruzi Adolescent Adult Aged Aged, 80 and over Argentina Chagas Cardiomyopathy Chagas Disease Child Child, Preschool DNA, Protozoan Endemic Diseases Female Genetic Variation Genotype Heart Humans Infant Male Middle Aged Phylogeny Polymerase Chain Reaction Trypanosoma cruzi Young Adult Trypanosoma cruzi |
| spellingShingle |
Chagas disease Discrete Typing Unit PCR Trypanosoma cruzi adolescent adult aged Argentina article bloodstream infection Chagas disease child chronicity endemic disease geographic distribution graft recipient human human tissue immunocompromised patient major clinical study pathogenesis polymerase chain reaction priority journal skin biopsy taxonomy tissue distribution Trypanosoma cruzi Adolescent Adult Aged Aged, 80 and over Argentina Chagas Cardiomyopathy Chagas Disease Child Child, Preschool DNA, Protozoan Endemic Diseases Female Genetic Variation Genotype Heart Humans Infant Male Middle Aged Phylogeny Polymerase Chain Reaction Trypanosoma cruzi Young Adult Trypanosoma cruzi Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| topic_facet |
Chagas disease Discrete Typing Unit PCR Trypanosoma cruzi adolescent adult aged Argentina article bloodstream infection Chagas disease child chronicity endemic disease geographic distribution graft recipient human human tissue immunocompromised patient major clinical study pathogenesis polymerase chain reaction priority journal skin biopsy taxonomy tissue distribution Trypanosoma cruzi Adolescent Adult Aged Aged, 80 and over Argentina Chagas Cardiomyopathy Chagas Disease Child Child, Preschool DNA, Protozoan Endemic Diseases Female Genetic Variation Genotype Heart Humans Infant Male Middle Aged Phylogeny Polymerase Chain Reaction Trypanosoma cruzi Young Adult Trypanosoma cruzi |
| description |
Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population. © Copyright 2012 Published by Cambridge University Press. |
| title |
Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| title_short |
Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| title_full |
Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| title_fullStr |
Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| title_full_unstemmed |
Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
| title_sort |
trypanosoma cruzi discrete typing units in chagas disease patients from endemic and non-endemic regions of argentina |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n4_p516_Cura http://hdl.handle.net/20.500.12110/paper_00311820_v139_n4_p516_Cura |
| _version_ |
1768542495073894400 |