Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics

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A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this c...

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Autores principales:	Torga, Jorge Román, Etcheverry, Javier Ignacio, Marconi, Mario Carlos
Publicado:	1997
Materias:	Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Laser pulses
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00304018_v143_n4-6_p230_Torga http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00304018_v143_n4-6_p230_Torga
record_format	dspace
spelling	paper:paper_00304018_v143_n4-6_p230_Torga2023-06-08T14:55:54Z Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics Torga, Jorge Román Etcheverry, Javier Ignacio Marconi, Mario Carlos Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Rotational depolarization Laser pulses A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V. Fil:Torga, J.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etcheverry, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marconi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00304018_v143_n4-6_p230_Torga http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Rotational depolarization Laser pulses
spellingShingle	Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Rotational depolarization Laser pulses Torga, Jorge Román Etcheverry, Javier Ignacio Marconi, Mario Carlos Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
topic_facet	Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Rotational depolarization Laser pulses
description	A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V.
author	Torga, Jorge Román Etcheverry, Javier Ignacio Marconi, Mario Carlos
author_facet	Torga, Jorge Román Etcheverry, Javier Ignacio Marconi, Mario Carlos
author_sort	Torga, Jorge Román
title	Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_short	Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_full	Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_fullStr	Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_full_unstemmed	Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_sort	design of a fluorescence technique using double laser pulse excitation for the measurement of molecular brownian dynamics
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00304018_v143_n4-6_p230_Torga http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
work_keys_str_mv	AT torgajorgeroman designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics AT etcheverryjavierignacio designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics AT marconimariocarlos designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics
_version_	1768542877597564928

Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics

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