The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons
The short interspersed repetitive element (SIRE) of Trypanosoma cruzi was first detected when comparing the sequences of loci that encode the TcP2β genes. It is present in about 1,500-3,000 copies per genome, depending on the strain, and it is distributed in all chromosomes. An initial analysis of S...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v97_n5_p2128_Vazquez http://hdl.handle.net/20.500.12110/paper_00278424_v97_n5_p2128_Vazquez |
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paper:paper_00278424_v97_n5_p2128_Vazquez |
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paper:paper_00278424_v97_n5_p2128_Vazquez2023-06-08T14:54:37Z The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons complementary DNA messenger RNA ribonuclease H RNA directed DNA polymerase amino acid sequence article controlled study DNA sequence genetic transcription long terminal repeat nonhuman nucleotide sequence polyadenylation priority journal retroposon reverse transcription polymerase chain reaction sequence homology short interspersed repeat species difference Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Consensus Sequence DNA, Protozoan Gene Expression Molecular Sequence Data Protozoan Proteins Retroelements Ribosomal Proteins Sequence Homology, Amino Acid Short Interspersed Nucleotide Elements Species Specificity Terminal Repeat Sequences Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi The short interspersed repetitive element (SIRE) of Trypanosoma cruzi was first detected when comparing the sequences of loci that encode the TcP2β genes. It is present in about 1,500-3,000 copies per genome, depending on the strain, and it is distributed in all chromosomes. An initial analysis of SIRE sequences from 21 genomic fragments allowed us to derive a consensus nucleotide sequence and structure for the element, consisting of three regions (I, II, and III) each harboring distinctive features. Analysis of 158 transcribed SIREs demonstrates that the consensus is highly conserved. The sequences of 51 cDNAs show that SIRE is included in the 3' end of several mRNAs, always transcribed from the sense strand, contributing the polyadenylation site in 63% of the cases. This study led to the characterization of VIPER (vestigial interposed retroelement), a 2,326-bp- long unusual retroelement. VIPER's 5' end is formed by the first 182 bp of SIRE, whereas its 3' end is formed by the last 220 bp of the element. Both SIRE moieties are connected by a 1,924-bp-long fragment that carries a unique ORF encoding a complete reverse transcriptase-RNase H gene whose 15 C- terminal amino acids derive from codons specified by SIRE's region II. The amino acid sequence of VIPER's reverse transcriptase-RNase H shares significant homology to that of long terminal repeat retrotransposons. The fact that SIRE and VIPER sequences are found only in the T. cruzi genome may be of relevance for studies concerning the evolution and the genome flexibility of this protozoan parasite. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v97_n5_p2128_Vazquez http://hdl.handle.net/20.500.12110/paper_00278424_v97_n5_p2128_Vazquez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
complementary DNA messenger RNA ribonuclease H RNA directed DNA polymerase amino acid sequence article controlled study DNA sequence genetic transcription long terminal repeat nonhuman nucleotide sequence polyadenylation priority journal retroposon reverse transcription polymerase chain reaction sequence homology short interspersed repeat species difference Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Consensus Sequence DNA, Protozoan Gene Expression Molecular Sequence Data Protozoan Proteins Retroelements Ribosomal Proteins Sequence Homology, Amino Acid Short Interspersed Nucleotide Elements Species Specificity Terminal Repeat Sequences Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
| spellingShingle |
complementary DNA messenger RNA ribonuclease H RNA directed DNA polymerase amino acid sequence article controlled study DNA sequence genetic transcription long terminal repeat nonhuman nucleotide sequence polyadenylation priority journal retroposon reverse transcription polymerase chain reaction sequence homology short interspersed repeat species difference Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Consensus Sequence DNA, Protozoan Gene Expression Molecular Sequence Data Protozoan Proteins Retroelements Ribosomal Proteins Sequence Homology, Amino Acid Short Interspersed Nucleotide Elements Species Specificity Terminal Repeat Sequences Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| topic_facet |
complementary DNA messenger RNA ribonuclease H RNA directed DNA polymerase amino acid sequence article controlled study DNA sequence genetic transcription long terminal repeat nonhuman nucleotide sequence polyadenylation priority journal retroposon reverse transcription polymerase chain reaction sequence homology short interspersed repeat species difference Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Consensus Sequence DNA, Protozoan Gene Expression Molecular Sequence Data Protozoan Proteins Retroelements Ribosomal Proteins Sequence Homology, Amino Acid Short Interspersed Nucleotide Elements Species Specificity Terminal Repeat Sequences Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
| description |
The short interspersed repetitive element (SIRE) of Trypanosoma cruzi was first detected when comparing the sequences of loci that encode the TcP2β genes. It is present in about 1,500-3,000 copies per genome, depending on the strain, and it is distributed in all chromosomes. An initial analysis of SIRE sequences from 21 genomic fragments allowed us to derive a consensus nucleotide sequence and structure for the element, consisting of three regions (I, II, and III) each harboring distinctive features. Analysis of 158 transcribed SIREs demonstrates that the consensus is highly conserved. The sequences of 51 cDNAs show that SIRE is included in the 3' end of several mRNAs, always transcribed from the sense strand, contributing the polyadenylation site in 63% of the cases. This study led to the characterization of VIPER (vestigial interposed retroelement), a 2,326-bp- long unusual retroelement. VIPER's 5' end is formed by the first 182 bp of SIRE, whereas its 3' end is formed by the last 220 bp of the element. Both SIRE moieties are connected by a 1,924-bp-long fragment that carries a unique ORF encoding a complete reverse transcriptase-RNase H gene whose 15 C- terminal amino acids derive from codons specified by SIRE's region II. The amino acid sequence of VIPER's reverse transcriptase-RNase H shares significant homology to that of long terminal repeat retrotransposons. The fact that SIRE and VIPER sequences are found only in the T. cruzi genome may be of relevance for studies concerning the evolution and the genome flexibility of this protozoan parasite. |
| title |
The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| title_short |
The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| title_full |
The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| title_fullStr |
The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| title_full_unstemmed |
The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons |
| title_sort |
short interspersed repetitive element of trypanosoma cruzi, sire, is part of viper, an unusual retroelement related to long terminal repeat retrotransposons |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v97_n5_p2128_Vazquez http://hdl.handle.net/20.500.12110/paper_00278424_v97_n5_p2128_Vazquez |
| _version_ |
1768542255859105792 |