Functional association between promoter structure and transcript alternative splicing
It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encode...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v94_n21_p11456_Cramer http://hdl.handle.net/20.500.12110/paper_00278424_v94_n21_p11456_Cramer |
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paper:paper_00278424_v94_n21_p11456_Cramer2023-06-08T14:54:36Z Functional association between promoter structure and transcript alternative splicing Fibronectin Transcription fibronectin rna polymerase ii alternative rna splicing article cell proliferation controlled study development gene structure hepatoma cell human human cell nonhuman priority journal promoter region rna transcription signal transduction spliceosome Alternative Splicing beta-Galactosidase Exons Fibronectins Globins Humans Models, Genetic Polymerase Chain Reaction Promoter Regions (Genetics) Recombinant Fusion Proteins RNA Polymerase II RNA Precursors RNA, Messenger Transcription, Genetic Transfection Tumor Cells, Cultured It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v94_n21_p11456_Cramer http://hdl.handle.net/20.500.12110/paper_00278424_v94_n21_p11456_Cramer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fibronectin Transcription fibronectin rna polymerase ii alternative rna splicing article cell proliferation controlled study development gene structure hepatoma cell human human cell nonhuman priority journal promoter region rna transcription signal transduction spliceosome Alternative Splicing beta-Galactosidase Exons Fibronectins Globins Humans Models, Genetic Polymerase Chain Reaction Promoter Regions (Genetics) Recombinant Fusion Proteins RNA Polymerase II RNA Precursors RNA, Messenger Transcription, Genetic Transfection Tumor Cells, Cultured |
| spellingShingle |
Fibronectin Transcription fibronectin rna polymerase ii alternative rna splicing article cell proliferation controlled study development gene structure hepatoma cell human human cell nonhuman priority journal promoter region rna transcription signal transduction spliceosome Alternative Splicing beta-Galactosidase Exons Fibronectins Globins Humans Models, Genetic Polymerase Chain Reaction Promoter Regions (Genetics) Recombinant Fusion Proteins RNA Polymerase II RNA Precursors RNA, Messenger Transcription, Genetic Transfection Tumor Cells, Cultured Functional association between promoter structure and transcript alternative splicing |
| topic_facet |
Fibronectin Transcription fibronectin rna polymerase ii alternative rna splicing article cell proliferation controlled study development gene structure hepatoma cell human human cell nonhuman priority journal promoter region rna transcription signal transduction spliceosome Alternative Splicing beta-Galactosidase Exons Fibronectins Globins Humans Models, Genetic Polymerase Chain Reaction Promoter Regions (Genetics) Recombinant Fusion Proteins RNA Polymerase II RNA Precursors RNA, Messenger Transcription, Genetic Transfection Tumor Cells, Cultured |
| description |
It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription. |
| title |
Functional association between promoter structure and transcript alternative splicing |
| title_short |
Functional association between promoter structure and transcript alternative splicing |
| title_full |
Functional association between promoter structure and transcript alternative splicing |
| title_fullStr |
Functional association between promoter structure and transcript alternative splicing |
| title_full_unstemmed |
Functional association between promoter structure and transcript alternative splicing |
| title_sort |
functional association between promoter structure and transcript alternative splicing |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v94_n21_p11456_Cramer http://hdl.handle.net/20.500.12110/paper_00278424_v94_n21_p11456_Cramer |
| _version_ |
1768542825134161920 |