Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms
A peptide from hindguts of the Triatoma hematophagous Chagas insect vector activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes to metacyclic trypomastigotes. Hindguts were obtained from insects fed 2 days earlie...
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1993
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v90_n21_p10140_Fraidenraich http://hdl.handle.net/20.500.12110/paper_00278424_v90_n21_p10140_Fraidenraich |
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paper:paper_00278424_v90_n21_p10140_Fraidenraich2023-06-08T14:54:35Z Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms Fraidenraich y Waisman, Diego Coso, Omar Adrian Baralle, Francisco Ernesto Torres, Héctor Norberto Flawiá, Mirtha María Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi adenylate cyclase hemoglobin a amino acid sequence amino terminal sequence article chagas disease controlled study enzyme activation epimastigote nonhuman parasite development parasite transmission priority journal triatoma trypanosoma cruzi trypomastigote Adenylate Cyclase Amino Acid Sequence Animal Chickens Chromatography, High Pressure Liquid Digestive Physiology Electrophoresis, Polyacrylamide Gel Globins Guanosine Triphosphate Guanylyl Imidodiphosphate Hemoglobins Human Mice Molecular Sequence Data Peptide Fragments Peptides Sequence Homology, Amino Acid Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi A peptide from hindguts of the Triatoma hematophagous Chagas insect vector activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes to metacyclic trypomastigotes. Hindguts were obtained from insects fed 2 days earlier with chicken blood. Purification was performed by gel filtration and HPLC on C18 and C4 columns. SDS/PAGE of the purified peptide showed a single band of about 10 kDa. The following sequence was determined for the 20 amino-terminal residues of this peptide: H 2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys- Ala-Ala-Ser-His. This sequence is identical to the amino terminus of chicken αD-globin. On a Western blot, the peptide immunoreacted with a polyclonal antibody against chicken globin D. A synthetic peptide corresponding to residues 1-40 of the αD-globin amino terminus also stimulated adenylyl cyclase activity and promoted differentiation. This 125I-labeled synthetic peptide bound specifically to T. cruzi epimastigote cells. Activation of epimastigote adenylyl cyclase by the hemoglobin-derived peptide may play an important role in T. cruzi differentiation and consequently in the transmission of Chagas disease. Fil:Fraidenraich, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baralle, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v90_n21_p10140_Fraidenraich http://hdl.handle.net/20.500.12110/paper_00278424_v90_n21_p10140_Fraidenraich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi adenylate cyclase hemoglobin a amino acid sequence amino terminal sequence article chagas disease controlled study enzyme activation epimastigote nonhuman parasite development parasite transmission priority journal triatoma trypanosoma cruzi trypomastigote Adenylate Cyclase Amino Acid Sequence Animal Chickens Chromatography, High Pressure Liquid Digestive Physiology Electrophoresis, Polyacrylamide Gel Globins Guanosine Triphosphate Guanylyl Imidodiphosphate Hemoglobins Human Mice Molecular Sequence Data Peptide Fragments Peptides Sequence Homology, Amino Acid Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi |
| spellingShingle |
Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi adenylate cyclase hemoglobin a amino acid sequence amino terminal sequence article chagas disease controlled study enzyme activation epimastigote nonhuman parasite development parasite transmission priority journal triatoma trypanosoma cruzi trypomastigote Adenylate Cyclase Amino Acid Sequence Animal Chickens Chromatography, High Pressure Liquid Digestive Physiology Electrophoresis, Polyacrylamide Gel Globins Guanosine Triphosphate Guanylyl Imidodiphosphate Hemoglobins Human Mice Molecular Sequence Data Peptide Fragments Peptides Sequence Homology, Amino Acid Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi Fraidenraich y Waisman, Diego Coso, Omar Adrian Baralle, Francisco Ernesto Torres, Héctor Norberto Flawiá, Mirtha María Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| topic_facet |
Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi Gallus gallus Hexapoda Insecta Triatoma Trypanosoma Trypanosoma cruzi adenylate cyclase hemoglobin a amino acid sequence amino terminal sequence article chagas disease controlled study enzyme activation epimastigote nonhuman parasite development parasite transmission priority journal triatoma trypanosoma cruzi trypomastigote Adenylate Cyclase Amino Acid Sequence Animal Chickens Chromatography, High Pressure Liquid Digestive Physiology Electrophoresis, Polyacrylamide Gel Globins Guanosine Triphosphate Guanylyl Imidodiphosphate Hemoglobins Human Mice Molecular Sequence Data Peptide Fragments Peptides Sequence Homology, Amino Acid Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi |
| description |
A peptide from hindguts of the Triatoma hematophagous Chagas insect vector activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes to metacyclic trypomastigotes. Hindguts were obtained from insects fed 2 days earlier with chicken blood. Purification was performed by gel filtration and HPLC on C18 and C4 columns. SDS/PAGE of the purified peptide showed a single band of about 10 kDa. The following sequence was determined for the 20 amino-terminal residues of this peptide: H 2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys- Ala-Ala-Ser-His. This sequence is identical to the amino terminus of chicken αD-globin. On a Western blot, the peptide immunoreacted with a polyclonal antibody against chicken globin D. A synthetic peptide corresponding to residues 1-40 of the αD-globin amino terminus also stimulated adenylyl cyclase activity and promoted differentiation. This 125I-labeled synthetic peptide bound specifically to T. cruzi epimastigote cells. Activation of epimastigote adenylyl cyclase by the hemoglobin-derived peptide may play an important role in T. cruzi differentiation and consequently in the transmission of Chagas disease. |
| author |
Fraidenraich y Waisman, Diego Coso, Omar Adrian Baralle, Francisco Ernesto Torres, Héctor Norberto Flawiá, Mirtha María |
| author_facet |
Fraidenraich y Waisman, Diego Coso, Omar Adrian Baralle, Francisco Ernesto Torres, Héctor Norberto Flawiá, Mirtha María |
| author_sort |
Fraidenraich y Waisman, Diego |
| title |
Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| title_short |
Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| title_full |
Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| title_fullStr |
Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| title_full_unstemmed |
Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms |
| title_sort |
stimulation of trypanosoma cruzi adenylyl cyclase by an αd-globin fragment from triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms |
| publishDate |
1993 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v90_n21_p10140_Fraidenraich http://hdl.handle.net/20.500.12110/paper_00278424_v90_n21_p10140_Fraidenraich |
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