Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. legumin...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v109_n30_p12135_Bonomi http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi |
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paper:paper_00278424_v109_n30_p12135_Bonomi2023-06-08T14:54:27Z Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor Posadas, Diana M. Zorreguieta, Angeles bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v109_n30_p12135_Bonomi http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum |
| spellingShingle |
bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum Posadas, Diana M. Zorreguieta, Angeles Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| topic_facet |
bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum |
| description |
Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum. |
| author |
Posadas, Diana M. Zorreguieta, Angeles |
| author_facet |
Posadas, Diana M. Zorreguieta, Angeles |
| author_sort |
Posadas, Diana M. |
| title |
Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| title_short |
Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| title_full |
Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| title_fullStr |
Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| title_full_unstemmed |
Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor |
| title_sort |
light regulates attachment, exopolysaccharide production, and nodulation in rhizobium leguminosarum through a lov-histidine kinase photoreceptor |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v109_n30_p12135_Bonomi http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi |
| work_keys_str_mv |
AT posadasdianam lightregulatesattachmentexopolysaccharideproductionandnodulationinrhizobiumleguminosarumthroughalovhistidinekinasephotoreceptor AT zorreguietaangeles lightregulatesattachmentexopolysaccharideproductionandnodulationinrhizobiumleguminosarumthroughalovhistidinekinasephotoreceptor |
| _version_ |
1768546104601739264 |