Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxy...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v91_n2_p359_Magnelli http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli |
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paper:paper_00275514_v91_n2_p359_Magnelli2023-06-08T14:54:17Z Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v91_n2_p359_Magnelli http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides |
| spellingShingle |
Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| topic_facet |
Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides |
| description |
Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars. |
| title |
Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| title_short |
Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| title_full |
Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| title_fullStr |
Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| title_full_unstemmed |
Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates |
| title_sort |
regulation of the cellulase complex production by saccobolus saccoboloides: induction and repression by carbohydrates |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v91_n2_p359_Magnelli http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli |
| _version_ |
1768542447043870720 |