Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia

In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translationelongation factor 1-α [tefA] and b-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies ob...

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Autores principales:	Iannone, Leopoldo Javier, Rossi, Maria Susana
Publicado:	2014
Materias:	Bromus setifolius Endophyte diversity Epichloë Neotyphodium Phleum alpinum Phylogeny genotype grass molecular analysis new species species diversity symbiosis Argentina Patagonia fungal DNA ribosomal spacer DNA article biodiversity classification endophyte diversity Epichloe fungus spore genetics growth, development and aging isolation and purification microbiology molecular genetics phylogeny physiology Poaceae Biodiversity DNA, Fungal DNA, Ribosomal Spacer Molecular Sequence Data Spores, Fungal Symbiosis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v106_n2_p339_McCargo http://hdl.handle.net/20.500.12110/paper_00275514_v106_n2_p339_McCargo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00275514_v106_n2_p339_McCargo2023-06-08T14:54:12Z Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia Iannone, Leopoldo Javier Rossi, Maria Susana Bromus setifolius Endophyte diversity Epichloë Neotyphodium Phleum alpinum Phylogeny genotype grass molecular analysis new species species diversity symbiosis Argentina Patagonia fungal DNA ribosomal spacer DNA article biodiversity Bromus setifolius classification endophyte diversity Epichloe Epichloë fungus spore genetics growth, development and aging isolation and purification microbiology molecular genetics Neotyphodium Phleum alpinum phylogeny physiology Poaceae symbiosis Bromus setifolius endophyte diversity Epichloë Neotyphodium Phleum alpinum phylogeny Biodiversity DNA, Fungal DNA, Ribosomal Spacer Epichloe Molecular Sequence Data Neotyphodium Phylogeny Poaceae Spores, Fungal Symbiosis In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translationelongation factor 1-α [tefA] and b-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species. © 2014 by The Mycological Society of America. Fil:Iannone, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v106_n2_p339_McCargo http://hdl.handle.net/20.500.12110/paper_00275514_v106_n2_p339_McCargo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bromus setifolius Endophyte diversity Epichloë Neotyphodium Phleum alpinum Phylogeny genotype grass molecular analysis new species species diversity symbiosis Argentina Patagonia fungal DNA ribosomal spacer DNA article biodiversity Bromus setifolius classification endophyte diversity Epichloe Epichloë fungus spore genetics growth, development and aging isolation and purification microbiology molecular genetics Neotyphodium Phleum alpinum phylogeny physiology Poaceae symbiosis Bromus setifolius endophyte diversity Epichloë Neotyphodium Phleum alpinum phylogeny Biodiversity DNA, Fungal DNA, Ribosomal Spacer Epichloe Molecular Sequence Data Neotyphodium Phylogeny Poaceae Spores, Fungal Symbiosis
spellingShingle	Bromus setifolius Endophyte diversity Epichloë Neotyphodium Phleum alpinum Phylogeny genotype grass molecular analysis new species species diversity symbiosis Argentina Patagonia fungal DNA ribosomal spacer DNA article biodiversity Bromus setifolius classification endophyte diversity Epichloe Epichloë fungus spore genetics growth, development and aging isolation and purification microbiology molecular genetics Neotyphodium Phleum alpinum phylogeny physiology Poaceae symbiosis Bromus setifolius endophyte diversity Epichloë Neotyphodium Phleum alpinum phylogeny Biodiversity DNA, Fungal DNA, Ribosomal Spacer Epichloe Molecular Sequence Data Neotyphodium Phylogeny Poaceae Spores, Fungal Symbiosis Iannone, Leopoldo Javier Rossi, Maria Susana Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
topic_facet	Bromus setifolius Endophyte diversity Epichloë Neotyphodium Phleum alpinum Phylogeny genotype grass molecular analysis new species species diversity symbiosis Argentina Patagonia fungal DNA ribosomal spacer DNA article biodiversity Bromus setifolius classification endophyte diversity Epichloe Epichloë fungus spore genetics growth, development and aging isolation and purification microbiology molecular genetics Neotyphodium Phleum alpinum phylogeny physiology Poaceae symbiosis Bromus setifolius endophyte diversity Epichloë Neotyphodium Phleum alpinum phylogeny Biodiversity DNA, Fungal DNA, Ribosomal Spacer Epichloe Molecular Sequence Data Neotyphodium Phylogeny Poaceae Spores, Fungal Symbiosis
description	In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translationelongation factor 1-α [tefA] and b-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species. © 2014 by The Mycological Society of America.
author	Iannone, Leopoldo Javier Rossi, Maria Susana
author_facet	Iannone, Leopoldo Javier Rossi, Maria Susana
author_sort	Iannone, Leopoldo Javier
title	Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
title_short	Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
title_full	Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
title_fullStr	Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
title_full_unstemmed	Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
title_sort	species diversity of epichloë symbiotic with two grasses from southern argentinean patagonia
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v106_n2_p339_McCargo http://hdl.handle.net/20.500.12110/paper_00275514_v106_n2_p339_McCargo
work_keys_str_mv	AT iannoneleopoldojavier speciesdiversityofepichloesymbioticwithtwograssesfromsouthernargentineanpatagonia AT rossimariasusana speciesdiversityofepichloesymbioticwithtwograssesfromsouthernargentineanpatagonia
_version_	1768543359059623936

Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia

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