Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To p...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v258_n1-2_p1_Rossi http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi |
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paper:paper_00268925_v258_n1-2_p1_Rossi2023-06-08T14:53:54Z Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems Abscisic acid (ABA) Asr genes Asr2 Drought stress Tomato abscisic acid beta glucuronidase article gene expression gene fusion multigene family nonhuman priority journal promoter region reporter gene tomato transcription regulation Carica papaya Embryophyta Lycopersicon esculentum Nicotiana tabacum Solanum tuberosum Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v258_n1-2_p1_Rossi http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Abscisic acid (ABA) Asr genes Asr2 Drought stress Tomato abscisic acid beta glucuronidase article gene expression gene fusion multigene family nonhuman priority journal promoter region reporter gene tomato transcription regulation Carica papaya Embryophyta Lycopersicon esculentum Nicotiana tabacum Solanum tuberosum |
| spellingShingle |
Abscisic acid (ABA) Asr genes Asr2 Drought stress Tomato abscisic acid beta glucuronidase article gene expression gene fusion multigene family nonhuman priority journal promoter region reporter gene tomato transcription regulation Carica papaya Embryophyta Lycopersicon esculentum Nicotiana tabacum Solanum tuberosum Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| topic_facet |
Abscisic acid (ABA) Asr genes Asr2 Drought stress Tomato abscisic acid beta glucuronidase article gene expression gene fusion multigene family nonhuman priority journal promoter region reporter gene tomato transcription regulation Carica papaya Embryophyta Lycopersicon esculentum Nicotiana tabacum Solanum tuberosum |
| description |
Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems. |
| title |
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| title_short |
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| title_full |
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| title_fullStr |
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| title_full_unstemmed |
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems |
| title_sort |
analysis of an abscisic acid (aba)-responsive gene promoter belonging to the asr gene family from tomato in homologous and heterologous systems |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v258_n1-2_p1_Rossi http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi |
| _version_ |
1768546664161738752 |