Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvatio...
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1977
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v156_n2_p229_deRivas http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas |
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paper:paper_00268925_v156_n2_p229_deRivas2023-06-08T14:53:53Z Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation amino acid beta galactosidase lactose messenger rna escherichia coli in vitro study microorganism operon rna synthesis starvation theoretical study Chromosomes, Bacterial Comparative Study Escherichia coli F Factor Genes Kinetics Lactose RNA, Messenger Translation, Genetic Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvation conditions employed during the induction period: 1. β-galactosidase synthesis was immediately obtained and a plateau was reached, in 20 min after restoration of growth, when cells had been induced during deprivation of amino-acids and carbon source. 2. β-galactosidase displayed an unusually long rate of synthesis and plateau was not reached before two doubling times, when cells had been induced during the deprivation of the sole amino-acids. The latter result points out a problem of messenger stability during those long translation kinetics and led us to study the behaviour of strains carrying lac genetic determinants on different replicative structures: chromosomic and plasmidic. In those two situations, induction of lac messenger RNA was obtained and ratify our previous observations. However, their translation kinetics suggest a DNA linkage of this induced messenger. © 1977 Springer-Verlag. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v156_n2_p229_deRivas http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amino acid beta galactosidase lactose messenger rna escherichia coli in vitro study microorganism operon rna synthesis starvation theoretical study Chromosomes, Bacterial Comparative Study Escherichia coli F Factor Genes Kinetics Lactose RNA, Messenger Translation, Genetic |
| spellingShingle |
amino acid beta galactosidase lactose messenger rna escherichia coli in vitro study microorganism operon rna synthesis starvation theoretical study Chromosomes, Bacterial Comparative Study Escherichia coli F Factor Genes Kinetics Lactose RNA, Messenger Translation, Genetic Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| topic_facet |
amino acid beta galactosidase lactose messenger rna escherichia coli in vitro study microorganism operon rna synthesis starvation theoretical study Chromosomes, Bacterial Comparative Study Escherichia coli F Factor Genes Kinetics Lactose RNA, Messenger Translation, Genetic |
| description |
Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvation conditions employed during the induction period: 1. β-galactosidase synthesis was immediately obtained and a plateau was reached, in 20 min after restoration of growth, when cells had been induced during deprivation of amino-acids and carbon source. 2. β-galactosidase displayed an unusually long rate of synthesis and plateau was not reached before two doubling times, when cells had been induced during the deprivation of the sole amino-acids. The latter result points out a problem of messenger stability during those long translation kinetics and led us to study the behaviour of strains carrying lac genetic determinants on different replicative structures: chromosomic and plasmidic. In those two situations, induction of lac messenger RNA was obtained and ratify our previous observations. However, their translation kinetics suggest a DNA linkage of this induced messenger. © 1977 Springer-Verlag. |
| title |
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| title_short |
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| title_full |
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| title_fullStr |
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| title_full_unstemmed |
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation |
| title_sort |
unusual stability and translation kinetics of an escherichia coli lac messenger rna synthetized during amino-acids deprivation |
| publishDate |
1977 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v156_n2_p229_deRivas http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas |
| _version_ |
1768542928620224512 |