Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic l...
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paper:paper_00257680_v66_n1_p9_Rodrigues2023-06-08T14:53:36Z Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells Elías, Fernanda Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic lymphocytic leukemia (CLL) cells. The surface molecules CD25, CD40, CD80 and CD86 were up-regulated upon incubation of the B-CLL cells with IMT504. Co-stimulation with IL-2 resulted in further up-regulation. IMT504-activated B-CLL cells were also good stimulators of T cells in allogeneic mixed lymphocyte reactions and co-stimulation with IL-2 improved this stimulation capacity. Apoptosis of the B-CLL cells in vitro was also stimulated by incubation with IMT504. In this case, co-stimulation with IL-2 was not significant. Furthermore, B-CLL cells of all the patients studied developed an immunogenic phenotype and entered stimulated apoptosis upon in vitro incubation with IMT504 independently of the mutational status of their 1gVH genes, becoming a good marker for tumor progression. Fil:Elias, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v66_n1_p9_Rodrigues http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction |
| spellingShingle |
Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction Elías, Fernanda Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| topic_facet |
Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction |
| description |
Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic lymphocytic leukemia (CLL) cells. The surface molecules CD25, CD40, CD80 and CD86 were up-regulated upon incubation of the B-CLL cells with IMT504. Co-stimulation with IL-2 resulted in further up-regulation. IMT504-activated B-CLL cells were also good stimulators of T cells in allogeneic mixed lymphocyte reactions and co-stimulation with IL-2 improved this stimulation capacity. Apoptosis of the B-CLL cells in vitro was also stimulated by incubation with IMT504. In this case, co-stimulation with IL-2 was not significant. Furthermore, B-CLL cells of all the patients studied developed an immunogenic phenotype and entered stimulated apoptosis upon in vitro incubation with IMT504 independently of the mutational status of their 1gVH genes, becoming a good marker for tumor progression. |
| author |
Elías, Fernanda |
| author_facet |
Elías, Fernanda |
| author_sort |
Elías, Fernanda |
| title |
Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| title_short |
Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| title_full |
Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| title_fullStr |
Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| title_full_unstemmed |
Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| title_sort |
oligonucleotide imt504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v66_n1_p9_Rodrigues http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues |
| work_keys_str_mv |
AT eliasfernanda oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells |
| _version_ |
1768542975275565056 |