Role of different forms of fibronectin in in vitro bovine follicular development

This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the...

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Autores principales:	Colman Lerner, Alejandro Ariel, Lanuza, Guillermo, Cramer, Paula, Kornblihtt, Alberto Rodolfo, Barañao, José Lino S.
Publicado:	1997
Materias:	fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00257680_v57_n3_p332_ColmanLerner
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spelling	paper:paper_00257680_v57_n3_p332_ColmanLerner2023-06-08T14:53:28Z Role of different forms of fibronectin in in vitro bovine follicular development Colman Lerner, Alejandro Ariel Lanuza, Guillermo Cramer, Paula Kornblihtt, Alberto Rodolfo Barañao, José Lino S. fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I+FN whereas total FN levels remained relatively constant. A negative correlation (P < 0.001) was detected between ED-I+FN and estradiol levels. This steroid was without effect on the alternative splicing of FN in primary cultures of bovine granulosa cells. However, cAMP produced a marked decrease in the incorporation of the ED-I region. In contrast, transforming growth factor beta (TGF-β) elicited both a stimulation on overall FN synthesis and an increase in the inclusion of ED-I. This effect was evident at the protein level (Western blots) and also in the mRNAs (Northern blots). A peptide corresponding to the ED-I region stimulated DNA synthesis in a bovine granulosa cell line (BGC-1) whereas the peptide corresponding to the flanking sequences was without effect. Data presented herein suggest a novel form of regulation by which changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development. Fil:Colman Lerner, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle
spellingShingle	fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle Colman Lerner, Alejandro Ariel Lanuza, Guillermo Cramer, Paula Kornblihtt, Alberto Rodolfo Barañao, José Lino S. Role of different forms of fibronectin in in vitro bovine follicular development
topic_facet	fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle
description	This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I+FN whereas total FN levels remained relatively constant. A negative correlation (P < 0.001) was detected between ED-I+FN and estradiol levels. This steroid was without effect on the alternative splicing of FN in primary cultures of bovine granulosa cells. However, cAMP produced a marked decrease in the incorporation of the ED-I region. In contrast, transforming growth factor beta (TGF-β) elicited both a stimulation on overall FN synthesis and an increase in the inclusion of ED-I. This effect was evident at the protein level (Western blots) and also in the mRNAs (Northern blots). A peptide corresponding to the ED-I region stimulated DNA synthesis in a bovine granulosa cell line (BGC-1) whereas the peptide corresponding to the flanking sequences was without effect. Data presented herein suggest a novel form of regulation by which changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development.
author	Colman Lerner, Alejandro Ariel Lanuza, Guillermo Cramer, Paula Kornblihtt, Alberto Rodolfo Barañao, José Lino S.
author_facet	Colman Lerner, Alejandro Ariel Lanuza, Guillermo Cramer, Paula Kornblihtt, Alberto Rodolfo Barañao, José Lino S.
author_sort	Colman Lerner, Alejandro Ariel
title	Role of different forms of fibronectin in in vitro bovine follicular development
title_short	Role of different forms of fibronectin in in vitro bovine follicular development
title_full	Role of different forms of fibronectin in in vitro bovine follicular development
title_fullStr	Role of different forms of fibronectin in in vitro bovine follicular development
title_full_unstemmed	Role of different forms of fibronectin in in vitro bovine follicular development
title_sort	role of different forms of fibronectin in in vitro bovine follicular development
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
work_keys_str_mv	AT colmanlerneralejandroariel roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment AT lanuzaguillermo roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment AT cramerpaula roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment AT kornblihttalbertorodolfo roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment AT baranaojoselinos roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
_version_	1768543883599282176

Role of different forms of fibronectin in in vitro bovine follicular development

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