A lineal equation for the classification of progressive and hyperactive spermatozoa

Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a...

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Autores principales: Vulcano, Gustavo José, Moses, Daniel Fernando, Valcarcel, Alberto, De Las Heras, Marcelo Antonio
Publicado: 1998
Materias:
article
fertilization
mathematical analysis
mathematical model
semen analysis
spermatozoon capacitation
spermatozoon maturation
spermatozoon migration
spermatozoon motility
spermatozoon penetration
Animals
Kinetics
Linear Models
Male
Mathematics
Sheep
Sperm Capacitation
Sperm Motility
Spermatozoa
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00255564_v149_n1_p77_Vulcano
http://hdl.handle.net/20.500.12110/paper_00255564_v149_n1_p77_Vulcano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00255564_v149_n1_p77_Vulcano
record_format dspace
spelling paper:paper_00255564_v149_n1_p77_Vulcano2023-06-08T14:53:09Z A lineal equation for the classification of progressive and hyperactive spermatozoa Vulcano, Gustavo José Moses, Daniel Fernando Valcarcel, Alberto De Las Heras, Marcelo Antonio article fertilization mathematical analysis mathematical model semen analysis spermatozoon capacitation spermatozoon maturation spermatozoon migration spermatozoon motility spermatozoon penetration Animals Kinetics Linear Models Male Mathematics Sheep Sperm Capacitation Sperm Motility Spermatozoa Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessment of capacitation in biochemical, physiological and clinical studies. In this study, we have applied a two- step heuristic model to deduce a lineal equation that discriminates hyperactive from progressive spermatozoa. The kinetic parameters (curvilinear velocity (VCL), linearity (LIN), amplitude of lateral head displacement (ALH), straightness (STR), wobble (WOB), mean 'dance' (DAN) and velocity of the average path (VAP)) of ram spermatozoa were evaluated with a computerized motility analyzer, and classified one by one as progressive or hyperactive by the appearance of their tracks. In a first step, a discriminating plane was defined by minimizing the number of misclassified spermatozoa ('conflicting points'); then, the plane was adjusted by an iterative process to minimize the distance from conflicting points to it. The resulting plane showed a discriminating capacity of over 95% for both classes, higher than that achieved by setting a threshold value for the parameters taken separately or in group. When included in a standard semen analysis, application of the equation allowed a rapid assessment of the percentage of hyperactive spermatozoa. The method described, developed in ram spermatozoa, can be applied to different species for a variety of purposes. Fil:Vulcano, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moses, D.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Valcárcel, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Las Heras, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00255564_v149_n1_p77_Vulcano http://hdl.handle.net/20.500.12110/paper_00255564_v149_n1_p77_Vulcano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic article
fertilization
mathematical analysis
mathematical model
semen analysis
spermatozoon capacitation
spermatozoon maturation
spermatozoon migration
spermatozoon motility
spermatozoon penetration
Animals
Kinetics
Linear Models
Male
Mathematics
Sheep
Sperm Capacitation
Sperm Motility
Spermatozoa
spellingShingle article
fertilization
mathematical analysis
mathematical model
semen analysis
spermatozoon capacitation
spermatozoon maturation
spermatozoon migration
spermatozoon motility
spermatozoon penetration
Animals
Kinetics
Linear Models
Male
Mathematics
Sheep
Sperm Capacitation
Sperm Motility
Spermatozoa
Vulcano, Gustavo José
Moses, Daniel Fernando
Valcarcel, Alberto
De Las Heras, Marcelo Antonio
A lineal equation for the classification of progressive and hyperactive spermatozoa
topic_facet article
fertilization
mathematical analysis
mathematical model
semen analysis
spermatozoon capacitation
spermatozoon maturation
spermatozoon migration
spermatozoon motility
spermatozoon penetration
Animals
Kinetics
Linear Models
Male
Mathematics
Sheep
Sperm Capacitation
Sperm Motility
Spermatozoa
description Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessment of capacitation in biochemical, physiological and clinical studies. In this study, we have applied a two- step heuristic model to deduce a lineal equation that discriminates hyperactive from progressive spermatozoa. The kinetic parameters (curvilinear velocity (VCL), linearity (LIN), amplitude of lateral head displacement (ALH), straightness (STR), wobble (WOB), mean 'dance' (DAN) and velocity of the average path (VAP)) of ram spermatozoa were evaluated with a computerized motility analyzer, and classified one by one as progressive or hyperactive by the appearance of their tracks. In a first step, a discriminating plane was defined by minimizing the number of misclassified spermatozoa ('conflicting points'); then, the plane was adjusted by an iterative process to minimize the distance from conflicting points to it. The resulting plane showed a discriminating capacity of over 95% for both classes, higher than that achieved by setting a threshold value for the parameters taken separately or in group. When included in a standard semen analysis, application of the equation allowed a rapid assessment of the percentage of hyperactive spermatozoa. The method described, developed in ram spermatozoa, can be applied to different species for a variety of purposes.
author Vulcano, Gustavo José
Moses, Daniel Fernando
Valcarcel, Alberto
De Las Heras, Marcelo Antonio
author_facet Vulcano, Gustavo José
Moses, Daniel Fernando
Valcarcel, Alberto
De Las Heras, Marcelo Antonio
author_sort Vulcano, Gustavo José
title A lineal equation for the classification of progressive and hyperactive spermatozoa
title_short A lineal equation for the classification of progressive and hyperactive spermatozoa
title_full A lineal equation for the classification of progressive and hyperactive spermatozoa
title_fullStr A lineal equation for the classification of progressive and hyperactive spermatozoa
title_full_unstemmed A lineal equation for the classification of progressive and hyperactive spermatozoa
title_sort lineal equation for the classification of progressive and hyperactive spermatozoa
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00255564_v149_n1_p77_Vulcano
http://hdl.handle.net/20.500.12110/paper_00255564_v149_n1_p77_Vulcano
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AT mosesdanielfernando linealequationfortheclassificationofprogressiveandhyperactivespermatozoa
AT valcarcelalberto linealequationfortheclassificationofprogressiveandhyperactivespermatozoa
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