Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis

Different cytochromes P450 are involved in steroid biosynthesis. These cytochromes have heme as the prosthetic group. We previously reported that ACTH, an activator of glucocorticoid biosynthesis in adrenal, requires heme biosynthesis for a maximal response. In the present study, we investigated the...

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Autores principales:	Martini, Claudia Noemí, Romero, Damián Gastón, Vila, María del Carmen
Publicado:	2007
Materias:	ACTH Ala-synthase (ALA-s) Cytochrome P450 21-hydroxylase (CYP21) Endothelin H295R human adrenocortical cells Low sodium diet Rat adrenal Steroid biosynthesis 5 aminolevulinate synthase corticosterone corticotropin cyclic AMP cytochrome P450 endothelin 1 messenger RNA mineralocorticoid steroid steroid 21 monooxygenase adrenal cell animal experiment animal tissue article controlled study enzyme activation enzyme induction heme synthesis hormonal regulation human human cell male nonhuman protein expression rat sodium restriction steroidogenesis 5-Aminolevulinate Synthetase Adrenal Cortex Adrenocorticotropic Hormone Aldosterone Animals Cell Line Corticosterone Diet, Sodium-Restricted Endothelin-1 Enzyme Induction Heme Humans Male Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v81_n1_p19_Martini http://hdl.handle.net/20.500.12110/paper_00243205_v81_n1_p19_Martini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00243205_v81_n1_p19_Martini
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spelling	paper:paper_00243205_v81_n1_p19_Martini2023-06-08T14:52:04Z Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis Martini, Claudia Noemí Romero, Damián Gastón Vila, María del Carmen ACTH Ala-synthase (ALA-s) Cytochrome P450 21-hydroxylase (CYP21) Endothelin H295R human adrenocortical cells Low sodium diet Rat adrenal Steroid biosynthesis 5 aminolevulinate synthase corticosterone corticotropin cyclic AMP cytochrome P450 endothelin 1 messenger RNA mineralocorticoid steroid steroid 21 monooxygenase adrenal cell animal experiment animal tissue article controlled study enzyme activation enzyme induction heme synthesis hormonal regulation human human cell male nonhuman protein expression rat sodium restriction steroidogenesis 5-Aminolevulinate Synthetase Adrenal Cortex Adrenocorticotropic Hormone Aldosterone Animals Cell Line Corticosterone Diet, Sodium-Restricted Endothelin-1 Enzyme Induction Heme Humans Male Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA Rattus Different cytochromes P450 are involved in steroid biosynthesis. These cytochromes have heme as the prosthetic group. We previously reported that ACTH, an activator of glucocorticoid biosynthesis in adrenal, requires heme biosynthesis for a maximal response. In the present study, we investigated the effect of ACTH, and the effect of two activators of the adrenal mineralocorticoid synthesis, endothelin-1 and low sodium diet on 5-aminolevulinate-synthase (ALA-s) mRNA. ALA-s is the rate-limiting enzyme in heme biosynthesis. It was found that infusion of rats with ACTH for 1 h caused an increase of adrenal ALA-s mRNA and activity accompanied by an increase in plasma corticosterone. CYP21, a cytochrome involved in the synthesis of both corticosterone and aldosterone, was not modified at the RNA level in adrenal glands by 1 h of ACTH infusion. Consistently, infusion of endothelin-1 for 1 h increased ALA-s mRNA and aldosterone content in adrenal gland without modifying CYP21 mRNA levels. To study if ALA-s is also regulated by the main physiological stimuli that increase adrenal mineralocorticoid secretion, we fed rats with low salt diet for 2 or 15 days. Low salt diet treatment increased adrenal gland ALA-s mRNA levels. On the other hand, the rapid stimulation of ALA-s mRNA by ACTH which acts through cyclic AMP was confirmed in H295R human adrenocortical cells, the only human adrenal cell line that has a steroid secretion pattern and regulation similar to primary cultures of adrenal cells. Our findings suggest that the acute activation of adrenal steroidogenic cytochromes by trophic hormones involves an increase in heme biosynthesis which will favor the production of active cytochromes. © 2007 Elsevier Inc. All rights reserved. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.d.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v81_n1_p19_Martini http://hdl.handle.net/20.500.12110/paper_00243205_v81_n1_p19_Martini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ACTH Ala-synthase (ALA-s) Cytochrome P450 21-hydroxylase (CYP21) Endothelin H295R human adrenocortical cells Low sodium diet Rat adrenal Steroid biosynthesis 5 aminolevulinate synthase corticosterone corticotropin cyclic AMP cytochrome P450 endothelin 1 messenger RNA mineralocorticoid steroid steroid 21 monooxygenase adrenal cell animal experiment animal tissue article controlled study enzyme activation enzyme induction heme synthesis hormonal regulation human human cell male nonhuman protein expression rat sodium restriction steroidogenesis 5-Aminolevulinate Synthetase Adrenal Cortex Adrenocorticotropic Hormone Aldosterone Animals Cell Line Corticosterone Diet, Sodium-Restricted Endothelin-1 Enzyme Induction Heme Humans Male Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA Rattus
spellingShingle	ACTH Ala-synthase (ALA-s) Cytochrome P450 21-hydroxylase (CYP21) Endothelin H295R human adrenocortical cells Low sodium diet Rat adrenal Steroid biosynthesis 5 aminolevulinate synthase corticosterone corticotropin cyclic AMP cytochrome P450 endothelin 1 messenger RNA mineralocorticoid steroid steroid 21 monooxygenase adrenal cell animal experiment animal tissue article controlled study enzyme activation enzyme induction heme synthesis hormonal regulation human human cell male nonhuman protein expression rat sodium restriction steroidogenesis 5-Aminolevulinate Synthetase Adrenal Cortex Adrenocorticotropic Hormone Aldosterone Animals Cell Line Corticosterone Diet, Sodium-Restricted Endothelin-1 Enzyme Induction Heme Humans Male Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA Rattus Martini, Claudia Noemí Romero, Damián Gastón Vila, María del Carmen Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
topic_facet	ACTH Ala-synthase (ALA-s) Cytochrome P450 21-hydroxylase (CYP21) Endothelin H295R human adrenocortical cells Low sodium diet Rat adrenal Steroid biosynthesis 5 aminolevulinate synthase corticosterone corticotropin cyclic AMP cytochrome P450 endothelin 1 messenger RNA mineralocorticoid steroid steroid 21 monooxygenase adrenal cell animal experiment animal tissue article controlled study enzyme activation enzyme induction heme synthesis hormonal regulation human human cell male nonhuman protein expression rat sodium restriction steroidogenesis 5-Aminolevulinate Synthetase Adrenal Cortex Adrenocorticotropic Hormone Aldosterone Animals Cell Line Corticosterone Diet, Sodium-Restricted Endothelin-1 Enzyme Induction Heme Humans Male Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA Rattus
description	Different cytochromes P450 are involved in steroid biosynthesis. These cytochromes have heme as the prosthetic group. We previously reported that ACTH, an activator of glucocorticoid biosynthesis in adrenal, requires heme biosynthesis for a maximal response. In the present study, we investigated the effect of ACTH, and the effect of two activators of the adrenal mineralocorticoid synthesis, endothelin-1 and low sodium diet on 5-aminolevulinate-synthase (ALA-s) mRNA. ALA-s is the rate-limiting enzyme in heme biosynthesis. It was found that infusion of rats with ACTH for 1 h caused an increase of adrenal ALA-s mRNA and activity accompanied by an increase in plasma corticosterone. CYP21, a cytochrome involved in the synthesis of both corticosterone and aldosterone, was not modified at the RNA level in adrenal glands by 1 h of ACTH infusion. Consistently, infusion of endothelin-1 for 1 h increased ALA-s mRNA and aldosterone content in adrenal gland without modifying CYP21 mRNA levels. To study if ALA-s is also regulated by the main physiological stimuli that increase adrenal mineralocorticoid secretion, we fed rats with low salt diet for 2 or 15 days. Low salt diet treatment increased adrenal gland ALA-s mRNA levels. On the other hand, the rapid stimulation of ALA-s mRNA by ACTH which acts through cyclic AMP was confirmed in H295R human adrenocortical cells, the only human adrenal cell line that has a steroid secretion pattern and regulation similar to primary cultures of adrenal cells. Our findings suggest that the acute activation of adrenal steroidogenic cytochromes by trophic hormones involves an increase in heme biosynthesis which will favor the production of active cytochromes. © 2007 Elsevier Inc. All rights reserved.
author	Martini, Claudia Noemí Romero, Damián Gastón Vila, María del Carmen
author_facet	Martini, Claudia Noemí Romero, Damián Gastón Vila, María del Carmen
author_sort	Martini, Claudia Noemí
title	Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
title_short	Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
title_full	Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
title_fullStr	Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
title_full_unstemmed	Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
title_sort	induction of 5-aminolevulinate synthase by activators of steroid biosynthesis
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v81_n1_p19_Martini http://hdl.handle.net/20.500.12110/paper_00243205_v81_n1_p19_Martini
work_keys_str_mv	AT martiniclaudianoemi inductionof5aminolevulinatesynthasebyactivatorsofsteroidbiosynthesis AT romerodamiangaston inductionof5aminolevulinatesynthasebyactivatorsofsteroidbiosynthesis AT vilamariadelcarmen inductionof5aminolevulinatesynthasebyactivatorsofsteroidbiosynthesis
_version_	1768543882660806656

Induction of 5-aminolevulinate synthase by activators of steroid biosynthesis

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