Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats
We studied the influence of maternal deprivation on the RNA biosynthesis in the brain cortex of 10 day-old rats. Mother-deprived pups, placed at 25°C showed a reduction in body temperature of 6 +/- 1°C. After mother retrieval, RNA biosynthesis decreased 27% and 34% in total brain cortex and in isola...
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1997
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paper:paper_00243205_v60_n24_p2165_Ibarra2023-06-08T14:52:02Z Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats Paratcha, Gustavo Christian Rodríguez, Jorge Andrés Azcurra, Julio Marcos brain cortex brain development cold stress maternal deprivation RNA biosynthesis nucleoside diphosphate kinase rna animal cell animal experiment animal tissue article body temperature brain cortex brain development cold stress controlled study enzyme activity maternal deprivation newborn nonhuman perikaryon rat rna synthesis Animals Animals, Newborn Body Temperature Regulation Cerebral Cortex Cold Kinetics Maternal Deprivation Rats Rats, Wistar RNA Stress We studied the influence of maternal deprivation on the RNA biosynthesis in the brain cortex of 10 day-old rats. Mother-deprived pups, placed at 25°C showed a reduction in body temperature of 6 +/- 1°C. After mother retrieval, RNA biosynthesis decreased 27% and 34% in total brain cortex and in isolated neurons, respectively. This fall is proportional to the body temperature reduction and can be avoided placing the pups at 37°C immediately after the separation. Rethermostatization of offsprings, after one hour at 25°C, showed an overshoot of RNA biosynthesis (145%) with further stabilization of synthesis rates to normal levels after 100 min. This classical physiological mechanism was further studied in vitro. Comparing in vive and in vitro experiments, it is concluded that overshooting can not be observed in vitro if temperature reduction was not previously performed in vivo. Thus, this phenomenon seems to respond to humoral factors in order to be triggered. Afterwards, in vitro overshooting following cold stress in vivo, demonstrates that the depressed tissue by itself has the capability to turn back to normal RNA levels in the same way as observed in vivo. Fil:Paratcha, G.Ch. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Azcurra, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v60_n24_p2165_Ibarra http://hdl.handle.net/20.500.12110/paper_00243205_v60_n24_p2165_Ibarra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
brain cortex brain development cold stress maternal deprivation RNA biosynthesis nucleoside diphosphate kinase rna animal cell animal experiment animal tissue article body temperature brain cortex brain development cold stress controlled study enzyme activity maternal deprivation newborn nonhuman perikaryon rat rna synthesis Animals Animals, Newborn Body Temperature Regulation Cerebral Cortex Cold Kinetics Maternal Deprivation Rats Rats, Wistar RNA Stress |
| spellingShingle |
brain cortex brain development cold stress maternal deprivation RNA biosynthesis nucleoside diphosphate kinase rna animal cell animal experiment animal tissue article body temperature brain cortex brain development cold stress controlled study enzyme activity maternal deprivation newborn nonhuman perikaryon rat rna synthesis Animals Animals, Newborn Body Temperature Regulation Cerebral Cortex Cold Kinetics Maternal Deprivation Rats Rats, Wistar RNA Stress Paratcha, Gustavo Christian Rodríguez, Jorge Andrés Azcurra, Julio Marcos Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| topic_facet |
brain cortex brain development cold stress maternal deprivation RNA biosynthesis nucleoside diphosphate kinase rna animal cell animal experiment animal tissue article body temperature brain cortex brain development cold stress controlled study enzyme activity maternal deprivation newborn nonhuman perikaryon rat rna synthesis Animals Animals, Newborn Body Temperature Regulation Cerebral Cortex Cold Kinetics Maternal Deprivation Rats Rats, Wistar RNA Stress |
| description |
We studied the influence of maternal deprivation on the RNA biosynthesis in the brain cortex of 10 day-old rats. Mother-deprived pups, placed at 25°C showed a reduction in body temperature of 6 +/- 1°C. After mother retrieval, RNA biosynthesis decreased 27% and 34% in total brain cortex and in isolated neurons, respectively. This fall is proportional to the body temperature reduction and can be avoided placing the pups at 37°C immediately after the separation. Rethermostatization of offsprings, after one hour at 25°C, showed an overshoot of RNA biosynthesis (145%) with further stabilization of synthesis rates to normal levels after 100 min. This classical physiological mechanism was further studied in vitro. Comparing in vive and in vitro experiments, it is concluded that overshooting can not be observed in vitro if temperature reduction was not previously performed in vivo. Thus, this phenomenon seems to respond to humoral factors in order to be triggered. Afterwards, in vitro overshooting following cold stress in vivo, demonstrates that the depressed tissue by itself has the capability to turn back to normal RNA levels in the same way as observed in vivo. |
| author |
Paratcha, Gustavo Christian Rodríguez, Jorge Andrés Azcurra, Julio Marcos |
| author_facet |
Paratcha, Gustavo Christian Rodríguez, Jorge Andrés Azcurra, Julio Marcos |
| author_sort |
Paratcha, Gustavo Christian |
| title |
Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| title_short |
Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| title_full |
Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| title_fullStr |
Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| title_full_unstemmed |
Cold stress related alteration of RNA biosynthesis in brain cortex of mother-deprived newborn rats |
| title_sort |
cold stress related alteration of rna biosynthesis in brain cortex of mother-deprived newborn rats |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v60_n24_p2165_Ibarra http://hdl.handle.net/20.500.12110/paper_00243205_v60_n24_p2165_Ibarra |
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AT paratchagustavochristian coldstressrelatedalterationofrnabiosynthesisinbraincortexofmotherdeprivednewbornrats AT rodriguezjorgeandres coldstressrelatedalterationofrnabiosynthesisinbraincortexofmotherdeprivednewbornrats AT azcurrajuliomarcos coldstressrelatedalterationofrnabiosynthesisinbraincortexofmotherdeprivednewbornrats |
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