Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain
We have previously reported that estrogen treatment of steroid-free, ovariectomized-adrenalectomized (OVX-ADX) rats, increased binding to glucocorticoid type II receptors (GR) in some brain regions. The present report studied the effects of estradiol in OVX-ADX rats receiving chronic corticosterone...
Guardado en:
| Publicado: |
1995
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n26_p2403_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v57_n26_p2403_Ferrini |
| Aporte de: |
| id |
paper:paper_00243205_v57_n26_p2403_Ferrini |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00243205_v57_n26_p2403_Ferrini2023-06-08T14:52:00Z Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain down regulation estradiol glucocorticoid glucocorticoid receptors immunocytochemistry 11beta,17beta dihydroxy 6 methyl 17alpha (1 propynyl)androsta 1,4,6 trien 3 one corticosterone dexamethasone estradiol glucocorticoid antagonist glucocorticoid receptor monoclonal antibody monoclonal antibody mab7 radioligand receptor subtype unclassified drug adrenalectomy animal experiment animal model animal tissue article brain region controlled study cytosol female hippocampus hypothalamus immunocytochemistry nonhuman oral drug administration ovariectomy rat receptor binding receptor down regulation septum nucleus stress subcutaneous drug administration thalamus midline nucleus Adrenalectomy Animal Antibodies, Monoclonal Brain Corticosterone Densitometry Down-Regulation Estradiol Female Glucocorticoids Immunoenzyme Techniques Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Support, Non-U.S. Gov't Animalia We have previously reported that estrogen treatment of steroid-free, ovariectomized-adrenalectomized (OVX-ADX) rats, increased binding to glucocorticoid type II receptors (GR) in some brain regions. The present report studied the effects of estradiol in OVX-ADX rats receiving chronic corticosterone (CORT) treatment. Using binding assays, GR was reduced by CORT replacement in cytosol of hippocampus and septum, but not in whole hypothalamus. GR were recovered after 4 days of estradiol therapy. Using Mab7, a monoclonal antibody against the activated nuclear form of GR, we observed that estrogen treatment increased immunoreactivity measured by computerized densitometry in areas targeted by glucocorticoids. Significantly higher staining for GR developed in CA1 and CA2 hippocampal subfields, paraventricular nucleus of the hypothalamus and lateral ventral septal nuclei of estradiol-receiving, CORT-treated OVX-ADX rats. The amplification of the glucocorticoid biological signal by female sex hormones, may thus affect several neuroendocrine parameters and the outcome of stressrelated diseases. © 1995. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n26_p2403_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v57_n26_p2403_Ferrini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
down regulation estradiol glucocorticoid glucocorticoid receptors immunocytochemistry 11beta,17beta dihydroxy 6 methyl 17alpha (1 propynyl)androsta 1,4,6 trien 3 one corticosterone dexamethasone estradiol glucocorticoid antagonist glucocorticoid receptor monoclonal antibody monoclonal antibody mab7 radioligand receptor subtype unclassified drug adrenalectomy animal experiment animal model animal tissue article brain region controlled study cytosol female hippocampus hypothalamus immunocytochemistry nonhuman oral drug administration ovariectomy rat receptor binding receptor down regulation septum nucleus stress subcutaneous drug administration thalamus midline nucleus Adrenalectomy Animal Antibodies, Monoclonal Brain Corticosterone Densitometry Down-Regulation Estradiol Female Glucocorticoids Immunoenzyme Techniques Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Support, Non-U.S. Gov't Animalia |
| spellingShingle |
down regulation estradiol glucocorticoid glucocorticoid receptors immunocytochemistry 11beta,17beta dihydroxy 6 methyl 17alpha (1 propynyl)androsta 1,4,6 trien 3 one corticosterone dexamethasone estradiol glucocorticoid antagonist glucocorticoid receptor monoclonal antibody monoclonal antibody mab7 radioligand receptor subtype unclassified drug adrenalectomy animal experiment animal model animal tissue article brain region controlled study cytosol female hippocampus hypothalamus immunocytochemistry nonhuman oral drug administration ovariectomy rat receptor binding receptor down regulation septum nucleus stress subcutaneous drug administration thalamus midline nucleus Adrenalectomy Animal Antibodies, Monoclonal Brain Corticosterone Densitometry Down-Regulation Estradiol Female Glucocorticoids Immunoenzyme Techniques Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Support, Non-U.S. Gov't Animalia Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| topic_facet |
down regulation estradiol glucocorticoid glucocorticoid receptors immunocytochemistry 11beta,17beta dihydroxy 6 methyl 17alpha (1 propynyl)androsta 1,4,6 trien 3 one corticosterone dexamethasone estradiol glucocorticoid antagonist glucocorticoid receptor monoclonal antibody monoclonal antibody mab7 radioligand receptor subtype unclassified drug adrenalectomy animal experiment animal model animal tissue article brain region controlled study cytosol female hippocampus hypothalamus immunocytochemistry nonhuman oral drug administration ovariectomy rat receptor binding receptor down regulation septum nucleus stress subcutaneous drug administration thalamus midline nucleus Adrenalectomy Animal Antibodies, Monoclonal Brain Corticosterone Densitometry Down-Regulation Estradiol Female Glucocorticoids Immunoenzyme Techniques Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Support, Non-U.S. Gov't Animalia |
| description |
We have previously reported that estrogen treatment of steroid-free, ovariectomized-adrenalectomized (OVX-ADX) rats, increased binding to glucocorticoid type II receptors (GR) in some brain regions. The present report studied the effects of estradiol in OVX-ADX rats receiving chronic corticosterone (CORT) treatment. Using binding assays, GR was reduced by CORT replacement in cytosol of hippocampus and septum, but not in whole hypothalamus. GR were recovered after 4 days of estradiol therapy. Using Mab7, a monoclonal antibody against the activated nuclear form of GR, we observed that estrogen treatment increased immunoreactivity measured by computerized densitometry in areas targeted by glucocorticoids. Significantly higher staining for GR developed in CA1 and CA2 hippocampal subfields, paraventricular nucleus of the hypothalamus and lateral ventral septal nuclei of estradiol-receiving, CORT-treated OVX-ADX rats. The amplification of the glucocorticoid biological signal by female sex hormones, may thus affect several neuroendocrine parameters and the outcome of stressrelated diseases. © 1995. |
| title |
Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| title_short |
Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| title_full |
Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| title_fullStr |
Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| title_full_unstemmed |
Estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| title_sort |
estradiol abolishes autologous down regulation of glucocorticoid receptors in brain |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n26_p2403_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v57_n26_p2403_Ferrini |
| _version_ |
1768544214302326784 |