Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis

The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mu...

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Autores principales:	Gliemmo, María Fernanda, Campos, Carmen Adriana
Publicado:	2015
Materias:	Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v64_n2_p794_Schelegueda http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00236438_v64_n2_p794_Schelegueda
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spelling	paper:paper_00236438_v64_n2_p794_Schelegueda2023-06-08T14:51:49Z Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis Gliemmo, María Fernanda Campos, Carmen Adriana Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Enterococcus mundtii Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes. © 2015 Elsevier Ltd. Fil:Gliemmo, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Campos, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v64_n2_p794_Schelegueda http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Enterococcus mundtii Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage
spellingShingle	Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Enterococcus mundtii Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage Gliemmo, María Fernanda Campos, Carmen Adriana Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
topic_facet	Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Enterococcus mundtii Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage
description	The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes. © 2015 Elsevier Ltd.
author	Gliemmo, María Fernanda Campos, Carmen Adriana
author_facet	Gliemmo, María Fernanda Campos, Carmen Adriana
author_sort	Gliemmo, María Fernanda
title	Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_short	Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full	Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_fullStr	Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full_unstemmed	Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_sort	synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by enterococcus mundtii isolated from odontesthes platensis
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v64_n2_p794_Schelegueda http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
work_keys_str_mv	AT gliemmomariafernanda synergisticantimicrobialactionandpotentialapplicationforfishpreservationofabacteriocinproducedbyenterococcusmundtiiisolatedfromodontesthesplatensis AT camposcarmenadriana synergisticantimicrobialactionandpotentialapplicationforfishpreservationofabacteriocinproducedbyenterococcusmundtiiisolatedfromodontesthesplatensis
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Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis

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