Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses
The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plant...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares http://hdl.handle.net/20.500.12110/paper_0022538X_v72_n1_p731_Ares |
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paper:paper_0022538X_v72_n1_p731_Ares2023-06-08T14:51:25Z Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses Ares, Vera Ximena Calamante, Gabriela Mentaberry, Alejandro Néstor gag protein article controlled study frameshift mutation nonhuman priority journal tobacco mosaic virus transgenic plant virus mutation virus resistance Animals Antibodies, Viral Base Sequence DNA Primers Frameshift Mutation Gene Expression Genetic Complementation Test Movement Open Reading Frames Phenotype Plants, Genetically Modified Plants, Toxic Potexvirus Rabbits Tobacco Tobacco Mosaic Virus Tobamovirus Viral Proteins The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in p24. Upon inoculation with tobacco mosaic virus (TMV), the size of necrotic local lesions was significantly smaller in p24+ plants than in nontransgenic, control plants. Systemic resistance to tobamoviruses was also evidenced after inoculation of p24+ plants with Ob, a virus that evades the hypersensitive response provided by the N gene. In the latter case, no systemic symptoms were observed, and virus accumulation remained low or undetectable by Western immunoblot analysis and back-inoculation assays. In contrast, no differences were observed in virus accumulation after inoculation with PVX, although more severe symptoms were evident on p24-expressing plants than on control plants. Similarly, infection assays conducted with potato virus Y showed no differences between control and transgenic plants. On the other hand, a considerable delay in virus accumulation and symptom development was observed when transgenic tobacco plants containing the movement protein (MP) of TMV were inoculated with PVX. Finally, a movement defective mutant of TMV was inoculated on p24+ plants or in mixed infections with PVX on nontransgenic plants. Both types of assays failed to produce TMV infections, implying that TMV MP is not interchangeable with the PVX MPs. Fil:Ares, X. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calamante, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares http://hdl.handle.net/20.500.12110/paper_0022538X_v72_n1_p731_Ares |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
gag protein article controlled study frameshift mutation nonhuman priority journal tobacco mosaic virus transgenic plant virus mutation virus resistance Animals Antibodies, Viral Base Sequence DNA Primers Frameshift Mutation Gene Expression Genetic Complementation Test Movement Open Reading Frames Phenotype Plants, Genetically Modified Plants, Toxic Potexvirus Rabbits Tobacco Tobacco Mosaic Virus Tobamovirus Viral Proteins |
| spellingShingle |
gag protein article controlled study frameshift mutation nonhuman priority journal tobacco mosaic virus transgenic plant virus mutation virus resistance Animals Antibodies, Viral Base Sequence DNA Primers Frameshift Mutation Gene Expression Genetic Complementation Test Movement Open Reading Frames Phenotype Plants, Genetically Modified Plants, Toxic Potexvirus Rabbits Tobacco Tobacco Mosaic Virus Tobamovirus Viral Proteins Ares, Vera Ximena Calamante, Gabriela Mentaberry, Alejandro Néstor Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| topic_facet |
gag protein article controlled study frameshift mutation nonhuman priority journal tobacco mosaic virus transgenic plant virus mutation virus resistance Animals Antibodies, Viral Base Sequence DNA Primers Frameshift Mutation Gene Expression Genetic Complementation Test Movement Open Reading Frames Phenotype Plants, Genetically Modified Plants, Toxic Potexvirus Rabbits Tobacco Tobacco Mosaic Virus Tobamovirus Viral Proteins |
| description |
The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in p24. Upon inoculation with tobacco mosaic virus (TMV), the size of necrotic local lesions was significantly smaller in p24+ plants than in nontransgenic, control plants. Systemic resistance to tobamoviruses was also evidenced after inoculation of p24+ plants with Ob, a virus that evades the hypersensitive response provided by the N gene. In the latter case, no systemic symptoms were observed, and virus accumulation remained low or undetectable by Western immunoblot analysis and back-inoculation assays. In contrast, no differences were observed in virus accumulation after inoculation with PVX, although more severe symptoms were evident on p24-expressing plants than on control plants. Similarly, infection assays conducted with potato virus Y showed no differences between control and transgenic plants. On the other hand, a considerable delay in virus accumulation and symptom development was observed when transgenic tobacco plants containing the movement protein (MP) of TMV were inoculated with PVX. Finally, a movement defective mutant of TMV was inoculated on p24+ plants or in mixed infections with PVX on nontransgenic plants. Both types of assays failed to produce TMV infections, implying that TMV MP is not interchangeable with the PVX MPs. |
| author |
Ares, Vera Ximena Calamante, Gabriela Mentaberry, Alejandro Néstor |
| author_facet |
Ares, Vera Ximena Calamante, Gabriela Mentaberry, Alejandro Néstor |
| author_sort |
Ares, Vera Ximena |
| title |
Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| title_short |
Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| title_full |
Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| title_fullStr |
Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| title_full_unstemmed |
Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses |
| title_sort |
transgenic plants expressing potato virus x orf2 protein (p24) are resistant to tobacco mosaic virus and ob tobamoviruses |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares http://hdl.handle.net/20.500.12110/paper_0022538X_v72_n1_p731_Ares |
| work_keys_str_mv |
AT aresveraximena transgenicplantsexpressingpotatovirusxorf2proteinp24areresistanttotobaccomosaicvirusandobtobamoviruses AT calamantegabriela transgenicplantsexpressingpotatovirusxorf2proteinp24areresistanttotobaccomosaicvirusandobtobamoviruses AT mentaberryalejandronestor transgenicplantsexpressingpotatovirusxorf2proteinp24areresistanttotobaccomosaicvirusandobtobamoviruses |
| _version_ |
1768544808612134912 |