Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa

The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were in...

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Detalles Bibliográficos
Publicado: 1984
Materias:
androgen
protein
animal cell
biological model
epididymis
female genital system
male genital system
nonhuman
spermatozoon
zona pellucida
Animalia
Cricetinae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p427_Cuasnicu
http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p427_Cuasnicu
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00224251_v71_n2_p427_Cuasnicu
record_format dspace
spelling paper:paper_00224251_v71_n2_p427_Cuasnicu2023-06-08T14:50:52Z Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa androgen protein animal cell biological model epididymis female genital system male genital system nonhuman spermatozoon zona pellucida Animalia Cricetinae The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were interpreted as meaning that de-novo protein synthesis was required to obtain the effect of androgens. When a fraction enriched in epididymal glycoproteins (EP2-EP6 (18% total protein in epididymal cytosol and 30% in enriched fraction) and depleted of androgens (<120 pg testosterone + DHT/ml) was added to the cultured epididymal tubules, the zona pellucida-binding ability of the contained spermatozoa increased from 0.55 in controls to 2.73 spermatozoa/egg in the extract-treated group (P<0.02). When the enriched fraction was prepared from epididymides of 30-day castrates, the stimulatory effect was lost (1.04 spermatozoa/egg). We suggest that proteins synthesized in the epididymis are required to obtain the effect of androgens and that the glycoproteins EP2-EP6 may be involved. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p427_Cuasnicu http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p427_Cuasnicu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic androgen
protein
animal cell
biological model
epididymis
female genital system
male genital system
nonhuman
spermatozoon
zona pellucida
Animalia
Cricetinae
spellingShingle androgen
protein
animal cell
biological model
epididymis
female genital system
male genital system
nonhuman
spermatozoon
zona pellucida
Animalia
Cricetinae
Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
topic_facet androgen
protein
animal cell
biological model
epididymis
female genital system
male genital system
nonhuman
spermatozoon
zona pellucida
Animalia
Cricetinae
description The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were interpreted as meaning that de-novo protein synthesis was required to obtain the effect of androgens. When a fraction enriched in epididymal glycoproteins (EP2-EP6 (18% total protein in epididymal cytosol and 30% in enriched fraction) and depleted of androgens (<120 pg testosterone + DHT/ml) was added to the cultured epididymal tubules, the zona pellucida-binding ability of the contained spermatozoa increased from 0.55 in controls to 2.73 spermatozoa/egg in the extract-treated group (P<0.02). When the enriched fraction was prepared from epididymides of 30-day castrates, the stimulatory effect was lost (1.04 spermatozoa/egg). We suggest that proteins synthesized in the epididymis are required to obtain the effect of androgens and that the glycoproteins EP2-EP6 may be involved.
title Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
title_short Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
title_full Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
title_fullStr Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
title_full_unstemmed Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
title_sort epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
publishDate 1984
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p427_Cuasnicu
http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p427_Cuasnicu
_version_ 1768545633655848960

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