STED nanoscopy with wavelengths at the emission maximum

Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate th...

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Publicado:	2016
Materias:	acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums Stimulated emission depletion Sub-diffraction Super resolution Fluorophores
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223727_v49_n36_p_Bordenave http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00223727_v49_n36_p_Bordenave
record_format	dspace
spelling	paper:paper_00223727_v49_n36_p_Bordenave2023-06-08T14:50:25Z STED nanoscopy with wavelengths at the emission maximum acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable. © 2016 IOP Publishing Ltd. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223727_v49_n36_p_Bordenave http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores
spellingShingle	acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores STED nanoscopy with wavelengths at the emission maximum
topic_facet	acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores
description	Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable. © 2016 IOP Publishing Ltd.
title	STED nanoscopy with wavelengths at the emission maximum
title_short	STED nanoscopy with wavelengths at the emission maximum
title_full	STED nanoscopy with wavelengths at the emission maximum
title_fullStr	STED nanoscopy with wavelengths at the emission maximum
title_full_unstemmed	STED nanoscopy with wavelengths at the emission maximum
title_sort	sted nanoscopy with wavelengths at the emission maximum
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223727_v49_n36_p_Bordenave http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave
_version_	1768546241324515328

STED nanoscopy with wavelengths at the emission maximum

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