Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)

Cell wall changes in vegetative and suffultory cells (SCs) and in oogonial structures from Oedogonium bharuchae N. D. Kamat f. minor Vélez were characterized using monoclonal antibodies against several carbohydrate epitopes. Vegetative cells and SCs develop only a primary cell wall (PCW), whereas ma...

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Autores principales: Estevez, Jose Manuel, Alberghina, Josefina Silvia
Publicado: 2008
Materias:
Cell wall
Cellulose
Green algae
HRGP
Oedogonium bharuchae f. minor
Oogonium development
Pectins
Chlorophyta
Embryophyta
Oedogoniales
Oedogonium bharuchae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v44_n5_p1257_Estevez
http://hdl.handle.net/20.500.12110/paper_00223646_v44_n5_p1257_Estevez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00223646_v44_n5_p1257_Estevez
record_format dspace
spelling paper:paper_00223646_v44_n5_p1257_Estevez2023-06-08T14:50:06Z Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta) Estevez, Jose Manuel Alberghina, Josefina Silvia Cell wall Cellulose Green algae HRGP Oedogonium bharuchae f. minor Oogonium development Pectins Chlorophyta Embryophyta Oedogoniales Oedogonium bharuchae Oedogonium bharuchae f. minor Cell wall changes in vegetative and suffultory cells (SCs) and in oogonial structures from Oedogonium bharuchae N. D. Kamat f. minor Vélez were characterized using monoclonal antibodies against several carbohydrate epitopes. Vegetative cells and SCs develop only a primary cell wall (PCW), whereas mature oogonial cells secrete a second wall, the oogonium cell wall (OCW). Based on histochemical and immunolabeling results, (1→4)-β-glucans in the form of crystalline cellulose together with a variable degree of Me-esterified homogalacturonans (HGs) and hydroxyproline-rich glycoprotein (HRGP) epitopes were detected in the PCW. The OCW showed arabinosides of the extensin type and low levels of arabinogalactan-protein (AGP) glycans but lacked cellulose, at least in its crystalline form. Surprisingly, strong colabeling in the cytoplasm of mature oogonia cells with three different antibodies (LM-5, LM-6, and CCRC-M2) was found, suggesting the presence of rhamnogalacturonan I (RG-I)-like structures. Our results are discussed relating the possible functions of these cell wall epitopes with polysaccharides and O-glycoproteins during oogonium differentiation. This study represents the first attempt to characterize these two types of cell walls in O. bharuchae, comparing their similarities and differences with those from other green algae and land plants. This work represents a contribution to the understanding of how cell walls have evolved from simple few-celled to complex multicelled organisms. © 2008 Phycological Society of America. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alberghina, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v44_n5_p1257_Estevez http://hdl.handle.net/20.500.12110/paper_00223646_v44_n5_p1257_Estevez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cell wall
Cellulose
Green algae
HRGP
Oedogonium bharuchae f. minor
Oogonium development
Pectins
Chlorophyta
Embryophyta
Oedogoniales
Oedogonium bharuchae
Oedogonium bharuchae f. minor
spellingShingle Cell wall
Cellulose
Green algae
HRGP
Oedogonium bharuchae f. minor
Oogonium development
Pectins
Chlorophyta
Embryophyta
Oedogoniales
Oedogonium bharuchae
Oedogonium bharuchae f. minor
Estevez, Jose Manuel
Alberghina, Josefina Silvia
Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
topic_facet Cell wall
Cellulose
Green algae
HRGP
Oedogonium bharuchae f. minor
Oogonium development
Pectins
Chlorophyta
Embryophyta
Oedogoniales
Oedogonium bharuchae
Oedogonium bharuchae f. minor
description Cell wall changes in vegetative and suffultory cells (SCs) and in oogonial structures from Oedogonium bharuchae N. D. Kamat f. minor Vélez were characterized using monoclonal antibodies against several carbohydrate epitopes. Vegetative cells and SCs develop only a primary cell wall (PCW), whereas mature oogonial cells secrete a second wall, the oogonium cell wall (OCW). Based on histochemical and immunolabeling results, (1→4)-β-glucans in the form of crystalline cellulose together with a variable degree of Me-esterified homogalacturonans (HGs) and hydroxyproline-rich glycoprotein (HRGP) epitopes were detected in the PCW. The OCW showed arabinosides of the extensin type and low levels of arabinogalactan-protein (AGP) glycans but lacked cellulose, at least in its crystalline form. Surprisingly, strong colabeling in the cytoplasm of mature oogonia cells with three different antibodies (LM-5, LM-6, and CCRC-M2) was found, suggesting the presence of rhamnogalacturonan I (RG-I)-like structures. Our results are discussed relating the possible functions of these cell wall epitopes with polysaccharides and O-glycoproteins during oogonium differentiation. This study represents the first attempt to characterize these two types of cell walls in O. bharuchae, comparing their similarities and differences with those from other green algae and land plants. This work represents a contribution to the understanding of how cell walls have evolved from simple few-celled to complex multicelled organisms. © 2008 Phycological Society of America.
author Estevez, Jose Manuel
Alberghina, Josefina Silvia
author_facet Estevez, Jose Manuel
Alberghina, Josefina Silvia
author_sort Estevez, Jose Manuel
title Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
title_short Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
title_full Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
title_fullStr Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
title_full_unstemmed Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
title_sort cell wall carbohydrate epitopes in the green alga oedogonium bharuchae f. minor (oedogoniales, chlorophyta)
publishDate 2008
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v44_n5_p1257_Estevez
http://hdl.handle.net/20.500.12110/paper_00223646_v44_n5_p1257_Estevez
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AT alberghinajosefinasilvia cellwallcarbohydrateepitopesinthegreenalgaoedogoniumbharuchaefminoroedogonialeschlorophyta
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